Health and Hygiene (Pty) Ltd. Reference Test Certificate Register for F10 Disinfectant Test description - testing body Test ref no.
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1 Reference Certificate Register for F10 Disinfectant ref no. certificate no. /date THHa1 n/a South African Inst Medical Research (SAIMR) 636 (1971) (99,9% kill) Mycobacterium terrae THHa2 n/a Medical and Pharmaceutical Services (MPS) AFNOR NF T (1988) (4 log Spectrum 5 bactericidal activity with interfering substances: P.aeruginosa, E.coli, S.aureus, Enterococcus faecium, Mycobacterium smegmatis THHa3 n/a MPS AFNOR NF T (1987) (4 log Spectrum 5 bactericidal activity P.aeruginosa, E.coli, S.aureus, Enterococcus faecium, Mycobacterium smegmatis: THHa4 n/a MPS AFNOR NF T (1987) (4 log Spectrum 5 bactericidal activity P.aeruginosa, E.coli, S.aureus, Enterococcus faecium, Mycobacterium smegmatis THHa5 n/a MPS AFNOR NF T (1987) (4 log Fungicidal activity: Penicillium verrucosum, Cladosporium cladosporoides, Absidia corymbifera, Candida albicans THHa6 n/a MPS AFNOR NF T (1986) (4 log Virucidal activity: Enterovirus, Orthopoxvirus, Adenovirus, HIV THHa7 1994/03/01 531/82355/L0376 1:25 1: :40 1: :40 1: South African Bureau of Standards () 636 (1971) (99,9%) E.coli, P.aeruginosa, S.aureus 5 THHa8 1994/04/13 Veterinary Institute, Onderstepoort: Canine parvovirus (>log 3 1:25 1: THHa9 1994/04/19 MPS Frosner, Jentsch and Ultheman (1982) Biocidal activity: Hepatitis B 1:25 1: THHa /04/19 MPS AFNOR NF T (1987) (4 log Leptospira, Campylobacter, Legionella THHa /04/20 MPS Summary of results plus toxicity, corrosion and biodegradability tests. THHa /05/06 Veterinary Institute, Onderstepoort Newcastle Disease Virus,Feline Herpes Virus (inactivation = Chlorox) 5 30
2 THHa /06/30 531/82533/L (1971) (99,9%) THHa /11/11 Eurostar Technology EU requirements Ecological impact Acinetobacter anitratus 5 zero zero continuous hazard hazard dosing THHa /12/09 Univ of Pretoria, Faculty Vet Science Dept Poultry Diseases IBD virus (complete inactivation) 1:50 1: THHa /02/13 Eurostar Technology Toxicity results: Oral and dermal LD 50, Ocular irritation, Draize test THHa16.a Eurostar Technology Acute Oral Toxicity s THHa16.b Eurostar Technology Acute Dermal Toxicity s THHa16.c Eurostar Technology Ocular Irritation s (Draize ) THHa /04/10 531/82879/M0620a 636 (1971) (99,9%) THHa /06/13 531/82946/M1026a THHa /12/13 Univ of Pretoria, Faculty Vet Science Dept Poultry Diseases THHa /01/08 531/83218/M2778e THHa /04/ /11/22 1:25 nil 4 & 2 1: mg/kg 1 & 24 hrs 1:25 1:125 LD mg/kg 1:50 1:250 LD50>4000mg/ kg 1:50 1:250 Score 4 at 1hr; 2 at 24hrs S.aureus (methicillin resistant) (1971) (99,9%) E.coli (HO157) 5 Ornithobacterium rhinotracheale (av 96,3%) 1: Kelsey Sykes Modified (5 clear tubes) Bacillus subtilus spores 1:30 1: (1971) (99,9%) Aspergillus niger 1:50 1: /83218/M2778a THHa /07/07 South African Vaccine Producers (SAIMR) 671 (1975) Primary Skin Irritation THHa /07/ /764861/P1806a 636 (1971) speed trials (99,9%) P.aeruginosa S.aureus 1: 50 THHa /01/03 Eurostar Technology Inhalation toxicity - acute and chronic nil nil THHa /01/04 Eurostar Technology Residuals on fruit and vegetables THHa /04/ /953611/Q907 1: & 48 hrs on intact and abraded skin 60 seconds 30 seconds from <0.1 to <0.5 ppm / cm (1971) (99,9%) Trichophyton mentogrophytes 15 THHa /10/08 Eurostar Technology Residual properties F10 and Chlorhexidene = = THHa /11/ / /Q Kelsey-Sykes (5 clear tubes) P.aeruginosa - dirty conditions (5% yeast) 1:40 1: min
3 THHa /02/ /S155 THHa /02/ / /R3632a THHa /03/ / /S5 THHa /03/ / /S846 THHa /03/ / /S1183a THHa /03/ / /S779 THHa /03/ / /S779a THHa /05/18 EEF000028D THHa37 THHa38 THHa39 THHa40 Biocidal test (bacteriastatic test) E.coli, P.aeruginosa, Klebsiella sp. 5 ppm 1 ppm (1971) (99,9%) Proteus vulgaris (no discolouration, pitting or etching) Aluminium corrosion test 1: 6,6 1: hrs hrs BS EN standard 1276 (1997) (10 5 log) P.aeruginosa, E.coli, S. aureus, Entercoccus hirae 5 pr EN standard 1276 (1997) (10 5 log) Clean and dirty 10 C and 20 C P.aeruginosa, E.coli, S. aureus, Entercoccus hirae Clean Dirty Clean Dirty 5 20 C 10 C <5 BS EN standard 1040 (1997) (10 5 log) P. aeruginous, S. aureus 5 : pr EN standard 1657 (1994) (10 4 log) Candida albicans 1: SAIMR 636 (1971) (99,9%) Salmonella typhi, 60 seconds Vancomycin resistant Enterococcus faecalis, 1: seconds Streptococcus pyogenes, 1: seconds Vibrio cholera 1: seconds See THHf1 See THHf2 See THHf3 See THHf4 THHa41 THHa /07/22 EEF00027X See THHf5 SAIMR 636 (1971) (99,9%) Listeria monocytogenes THHa43a 1997/12/18 Glaxowelcome S. epidermides, P. cepacia, Microccus luteus, Salmonella abony, Klebsiella pneumoniae, Corynebacterium xerosis, C. albicans, 1: :25 1: seconds 60 seconds Spores 1 hour
4 ref no. certificate no. /date THHa43b Glaxowelcome S. epidermides, P. aeruginosa, P. (Burkholderia) cepacia, Micrococcus luteus, Salmonella abony, Citrobacter freundii, entrobacter sakazakii, Klebsiella pneumoniae, E. coli, Corynebacterium xerosis, C. albicans,, Entrobacter cloacae, A. niger THHa / / S4314a THHa / /T (1954) Corrosiveness (6.10) Rinsing properties (6.11) Water insoluble matter content (6.12) Storage stability (6.13) Minimum Inhibitory Concentration (MIC) S. aureus P. aeruginosa 1:25 1:125 1:20 1:20 between 1:6553/ 13,107 1: 102/ 204 1:5 1:5 between 1:32768/6 5,536 1:512/ 1024 THHa Clover SA Inhibitory Substances Screening B.stearothermophilus 1:50 1:250 THHa / /T879 THHa cross ref THHd2 THHa / /T THHa / /T THHa DT/fm HH09/00 cross ref THHa16 Giescke, WH. Van Den Heever, LW. (1971) Udder disinfectant P. aeruginosa, S. aureus 1:50 1:250 SAIMR 671 (1975) Primary Skin Irritation 1:25 1: (1971) (99,9%) P.aeruginosa 636 (1971) (99,9%) P.aeruginosa Eurostar Technology Oral and topical toxicity (LD50) >5000 mg/kg THHa Rainbow Farms, Hammersdale Lab Malthus 2000 Analyser THHa /82881/M0634 THHa /2000 >5000 mg/kg E.coli strain of avian origin 1:300 1:1500 Effect of irradiation on bactericidal activity P.aeruginosa Poultry Reference Laboratory Newcastle Disease Virus Spores 60 passes passes 0,3g/l passes passes 22 for 48 hrs 3 63 Dilutions with milk of 1:20 upwards are negative 0.5, 1, and & 48 hrs on intact and abraded skin 1 year stability test 2 year stability test as sold product concentrate 15 minute contact No dimunit-ion of perfor-mance at levels from 4kGy to 25kGy in 20
5 THHa /2000 Poultry Reference Laboratory Newcastle Disease Virus THHa ARC- Onderstepoort Veterinary Institute Rabies Unit - Rabies Virus Between 1:150-1:300 Between 1:750-1:1500 in 20 C Between - 1:150 Between1: 10 C THHa57a ARC - Animal Improvement Institute Skin sensitivity trial-highveld winter conditions,using as an udder wash THHa57b See THHaa5 THHa Poultry Reference Laboratory Infectious Bursal Disease Virus (IBD) THHa59 See THHf6 THHa Keymed Compatibility of F10 Disinfectant Solution with Olympus Flexible Endoscopes THHa See THHf7 THHa See THHg1 FMD Disinfectant 1: 50 1:250 No sensitivity - zero score 1: 50 1:250 in Use in UK Market THHa / /926 /T5930 THHa See THHf9 - Fitosan 1593 (1994): Disinfectants based on Gluteraldehyde for use on medical instruments - Kelsey Sykes (modified) test using B.subtilis spores 1:20 Clean test: 5 clear tubes in 1 hour THHa65 THHa66 THHa67 THHa68 THHa69 THHa / /U See THHg2 FMD Disinfectant See THHg3 FMD Corrosion See THHg4 FMD Corrosion 636(1971) (99.9%) S aureus See THHf10 - Fitosan See THHg5 FMD Corrosion THHa Univ. of Pretoria Faculty of Biological & Agricultural Science. as a mist spray at 125ppm Aspergillus fumigatus THHa / /V (1971) (99,9%) Pasteurella multocida THHa Dr I.M. Petzer (U.P Faculty of Veterinary Science) Field Trial Pseudo-cowpox (See also THHh3) 1:50 1:250 > 99.9% kill of S.aureus within 15 sec 1:1600 1:8000 No spore germination on plate, even after 5 days 5 min Passed 1:50 1: day Supervised trial 1.2% reinfection
6 THHa /02/V a Clostridium sporogenes SATCC C1 29 1:50 1:250 % kill: 10 min % 20 min 98% THHa / /W (1971) (99.9%) Pseudomonas Aeruginosa THHa Eurostar Technology Limited THHa / / W A THHa / /W4204 A THHa / /W4203 A THHa / /X20316 THHa81a / /W Chemical incompatibility between aldehyde products and F10 solutions. 636 (1971) (99.9%) Pseudomonas Aeruginosa 636 Corrosion test for Mark Scheme 636/639 Efficacy and corrosion test for Mark Scheme SANS Salmonella choleraesuis typhimurium ATCC Sal 12 SANS Pseudomonas aeruginosa SATCC Psc 16 1:50 1:250 Ready to use solution tested over a 6 month period without fall in efficiency performance. - - F10 Solution is incompatible with any aldehyde based products and will result in reddish brown deposits on instruments / surfaces. Stability tests on batches from 2001, 2002, 2003 all comply. F10SC Passes F10SCXD Passes > 99.9 % kill in 2 1:50 1:250 > 99.9 % kill in 2 min and 5 min ed every month over a 24 month period. Samples stored at 37 C throughout. THHa81b / /X50945 THHa /04 SANS Pseudomonas aeruginosa SATCC Psc 16 Micro Laboratorie, Karachi, Pakistan Cocktail of gram positive and gram negative bacteria. 1:50 1: % kill in 2 min and 5 min of 2 year old retention sample. 1:200 0 Log 7 reduction in 5
7 THHa Lab Ref. No. 607 THHa / /X21367 THHa / /X31628a THHa / /X31628b THHa / /X31628c THHa / /X THHa89a / 04-5 /X18434 K & Ns Poultry Disease Diagnostic & Research Institute FDA/BAM Bactericidal Activity Poultry pathogenic strain of E.Coli Staphylococci Pseudomonas C. albicans SANS Trichophyton mentagrophytes EN EN Aspergillus niger spores EN Aspergillus niger spores EN Simulating cleaning and disinfection cycles a) utilising an ultrasonic bath in the cleaning cycle. Complete inhibition 1:50 1: % kill in 10 1: % kill in 5 1: % kill in 5 1: % kill in 5 1: % kill in 5 1: % kill in in total elapse time of 12 (Refer THHa85). THHa89b / /W / 9a THHa / /X34736/40 Simulating cleaning and disinfection cycles b) utilising a mechanical agitator in the cleaning cycle. Staphylococcus epidermidis using a commercial pressurised aerosol can (average droplet size, 26 microns) THHa University of Pretoria Department of Veterinary Tropical Diseases, Onderstepoort. Use of F10SC on superficial tissues (fibroblas-like cells) oaskin and open wounds. Borine dermis cells 1:400 1:2000 THHa Onderstepoort Veterinary Institute Avian Influenza Virus (H5N2 HPAI) 1: % kill in in total elapse time of 12 (Refer THHa85). 1: % kill in 10 (no survivors) 30 seconds cells unaffected. 5 50% cells remain viable. NB: After 24hrs results unchanged. 10 complete inactivation
Health and Hygiene (Pty) Ltd. Reference Test Certificate Register for F10 Disinfectant Test description - testing body Test ref no.
Reference Test Certificate Register for F10 Disinfectant Test ref no. Test certificate no. /date THHa1 n/a South African Inst Medical Research (SAIMR) 636 (1971) (99,9% kill) Mycobacterium terrae THHa2
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