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1 16026 University Oak, San Antonio, TX geneontechnologies.com **NOTE: This is a comprehensive report of GenEon s On-Site Generator Microbiological Kill Claims. Table 1 The mycobacteriocidal efficacy of the liquid generated by GenEon s On-Site Generators (BCS )1 at 30 second contact time at 412 ppm of FAC. Challenge Mycobacterium terrea slide # Cumulative Cumulative Log Slide 1 <0.4 Slide 2 <0.4 Slide x > % >.8 Slide 4 <0.4 Slide <0.4 Table 2 The bactericidal efficacy of the liquid generated by GenEon s On-Site Generators; Germicidal Spray Products as Disinfectants (200) using a 30 second contact Inoculated Slides (number of replicates tested) time at 30 ppm of FAC. microorganism slide # Log Bacteriophage MS x <.91 > % > x <.91 > % >.
2 Table 3 The virucidal efficacy of the liquid generated by GenEon s On-Site Generators against at various contact times using 800 ppm of FAC. Test was conducted as per AOAC Official Method ; Germicidal Spray Products as Disinfectants (200) and ASTM E3 Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces Number of replicates tested Contact Time (Minutes) Control Slide Infectious Units (iu) /ml # iu/ml Recovered From Each Slide* Log x x x % 4.2 >99.998% >4.8 >99.999% >.0 Table 4 ASTM E (2008) NG3611, Assessment of Antimicrobial Activity Using a GenEon On-Site Generator for time Kill Procedure. C. difficile ATCC 4398 (endospores) Contact Time Starting Concentration minutes Test Substance PBS Control 800 ppm Chlorine solution (FAC) Replicate CFU/mL E E E+0 1 <0 2 <0 3 <0 CFU/mL 8.00E+0 from Starting Concentration N/A <0 >99.994%
3 Table C. difficile ATCC 4398 (endospores) Ave rag e CF U/ ml 1.00E E E E E E E E+00 PBS Control Starting Concentration 800 ppm Chlorine solution (FAC ) minutes Table 6 The bactericidal efficacy of the liquid generated by GenEon s On-Site Generators. Test was conducted as per AOAC Official Method ; Germicidal Spray Products as Disinfectants at 20 ppm of FAC (200) Inoculated Slides Tubes Demonstrating Growth Positive Control (unsprayed slide) Negative Control (uninoculated slide) Staphylococcus aureus (MRSA) Salmonella enterica None Growth No-Growth None Growth No-Growth None Growth No-Growth None Growth No-Growth E. coli O17:H7 None Growth No-Growth
4 Table 7 The bactericidal efficacy of the liquid generated by the GenEon On-Site Generator at 20 ppm of FAC. Inoculated Slides (number of replicates tested) microorganism slide# Log Staphylococcus aureus (MRSA) >1.0 x >99.999% >.0 Salmonella enterica >1.0 x >99.999% >.0 >1.0 x >99.999% >.0 >1.0 x >99.999% >.0 E. coli O17:H7 >1.0 x >99.999% >.0 Table 8 The virucidal efficacy of the liquid generated by GenEon s On-Site Generators. Test was conducted as per AOAC Official Method ; Germicidal Spray Products as Disinfectants (200) and ASTM E3 Standard Test Method for Efficacy of Virucidal at 20 ppm of FAC. Inoculated Slides (number of replicates tested) infectious particles (iu) /ml slide# iu/ml each of slides Log Poliovirus CHAT Lsc1 4.6 x 4 <0. >99.999% > x <0. > % >6.0
5 Table 9 Inactivation of E. coli O17:H7 and at the indicated time points following introduction to the liquid produced by GenEon s On-Site Generators at 20 ppm of FAC. Bacterial at the indicated time points following study start 1 0 (control) minutes Control (Final) E. coli O17:H7 2.9 x 6 ) or >6 Log 2.9 x x 6 ) or >6 Log 8.9 x
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