MCB 301- BACTERIOLOGY COURSE PARTICULARS COURSE INSTRUCTORS COURSE DESCRIPTION

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1 MCB 301- BACTERIOLOGY COURSE PARTICULARS Course Code: MCB 301 Course Title: BACTERIOLOGY No. of Units: 3 Course Duration: Two hours of theory and three hours of practicals per week for 15 weeks. Status: Compulsory Course Address: mcb301@gmail.com Course Webpage: Prerequisite: MCB201, MCB202. COURSE INSTRUCTORS Dr. (Mrs)A.O. Ogundare Dept. of Microbiology, Federal University of Technology, Akure, Nigeria. Phone: aoogundare@futa.edu.ng COURSE DESCRIPTION The course is a prerequisite of General Microbiology 1 and II (MCB201) and (MCB202).This course explains the history of bacteria, structure and functions of parts of the structure. It lets students know more about the bacterium as a living organism that has size, shape, metabolizes, feeds and grows although they are miniature in nature and cannot be seen with the naked eye.the student is exposed to the knowledge of the mode of nutrition in bacteria, the growth curve including the lag, log, stationary and decline phases. The students will be able to calculate the generation time for a bacterium and know that it differences from one bacterium to the other. This course further introduces and exposes the students to the knowledge of pathogenicity in bacteria. They will know what pathogens are, some common bacterial diseases and their causative organisms, the sources, symptoms, treatment and control of the infection. They will be able to know the different sources for the isolation of different bacteria. The Koch s postulate is presented to the students as an experimental procedure for isolating and identifying organisms responsible for particular diseases. Students will know theoretically and practically, how to isolate bacterial pathogens. 1

2 COURSE OBJECTIVES The objectives of this course are to: explain what bacteriology is; introduce students to the structure and of bacteria, nutrition and growth in bacteri teach them what pathogenicity means, introduce the students to some pathogenic bacteria, where they can be found (i.e. their sources), diseases they cause, symtoms, treatment and control of such diseases. provide students with opportunities to isolate and identify these bacterial isolates. introduce to students the Koch s postulates and its application in determining the pathogenicity of an organism COURSE LEARNING OUTCOMES / COMPETENCIES Upon successful completion of this course, the student will be able to know: (Knowledge based) what bacteriology means; the structure of bacteria; nutrition and growth in bacteria; the difference between pathogenic and non pathogenic bacteria; some common pathogenic bacteria and their sources; diseases caused by these pathogens, and symptoms; and treatments and control measures available for various bacterial infections. (Skills) how to carefully handle pathogenic bacteria while isolating and identifying them; where and how to isolate a bacterium of choice; how to identify bacteria; and how to confirm the pathogenicity of a particular bacterium GRADING SYSTEM FOR THE COURSE This course will be graded as follows: Class Attendance 05% Assignments 05% Test(s) 10% Practicals 20% Final Examination 60% TOTAL 100% 2

3 GENERAL INSTRUCTIONS Attendance: It is expected that every student will be in class for lectures and also participate in all practical exercises. Attendance records will be kept and used to determine each person s qualification to sit for the final examination. In case of illness or other unavoidable cause of absence, the student must communicate as soon as possible with me, indicating the reason for the absence. Academic Integrity: Violations of academic integrity, including dishonesty in assignments, examinations, or other academic performances are prohibited. You are not allowed to make copies of another person s work and submit it as your own; that is plagiarism. All cases of academic dishonesty will be reported to the University Management for appropriate sanctions in accordance with the guidelines for handling students misconduct as spelt out in the Students Handbook. Assignments and Group Work: Students are expected to submit assignments as scheduled. Failure to submit an assignment as at when due will earn you zero for that assignment. Only under extenuating circumstances, for which a student has notified me in advance, will late submission of assignments be permitted. Code of Conduct in Lecture Rooms and Laboratories: Students should turn off their cell phones during lectures. Students are prohibited from engaging in other activities (such as texting, watching videos, etc.) during lectures. Food and drinks are not permitted in the laboratories. READING LIST 1 Willey, J.M., Sherwood, M.L., and Woolverton, J. C. (2008) Prescott, Harley, and Klein s Microbiology. 7th ed. Mc Graw-Hill Inc, NY. 2 Michael J. Pelczar,JR., E.C.S. Chan and Noel R. Krieg. (1993) Microbiology. 5th ed.tata McGraw-Hill New Delhi. 3 Tortora, G,J., Funke, B.R., and Case, C.L. (2013) Microbiology: an introduction. 11th ed. Pearson publishers, NY. 4 Fawole, M.O. and Oso, B.A. (2001). Laboratory manual of Microbiology. Spectrum books Limited, Ibadan, Nigeria. 5 Olutiola, P.O., O. Famurewa and Sonntag HG (1991). An Introduction to Microbiology: A Practical Approach.Ist Edition, Hygieneinstitutder Universitat Heidelberg,Germany. 6 Micheal. T. Madigan and John M. Martinko (2006). Brock Biology of Microorganisms. 11th ed. Pearsons Prentice hall NJ. Legend 1- Available in the University Library 2- Available in Departmental/School Libraries 3- Available on the Internet. 4- Available as Personal Collection 5- Available in local bookshops. 3

4 COURSE OUTLINE Week Topic Remarks 1 Introduction and Course Overview During this first class, the course contents will be discussed with the students, with brief explanation on each of the topics. The expectation of the students from the course will also 2 & 3 Historical perspectives of bacteriology Discovery of microorganisms Historical perspectives of bacteriology Definition of bacteriology Cultivation of bacteria be documented. Students will know the importance of bacteria in nature, and that they are ubiquitous. They will be taught how to safely handle pathogenic bacteria. Students will be given various examples of bacteria, pathogenic, opportunistic and beneficiary hence they will know that bacteria are not all enemies but can be our friends 4 & 5 The morphology and fine structure of Bacteria Size, shape and arrangements of bacterial cells Bacterial structures and function external structure to cell wall internal structures the bacterial cell wall (composition, Gram- Positive and Gram -Negative) Relationship between size and metabolism of bacteria The bacterial structure will be explained to the students. The students will have the knowledge of both the structure and function of the bacterial structure. Students will know the peptidoglycan structure of the bacterial cell wall. 6&7 NUTRITION IN BACTERIA Requirements for Carbon, Oxygen, Hydrogen, and electrons macronutrients (C, O, H, S, P, N, K +, Ca 2+, Mg 2+, Fe 2+ and Fe 3+ ) micronutrients or trace elements (Mn 2+, Zn, Co 2+, Mo 2+, Cu, e.t.c) Nutritional types of Microorganisms Students will understand the various nutritional requirements of bacteria, the macro and trace elements and how they are obtained. 8 & 9 BACTERIAL GROWTH normal growth cycle of bacteria (the growth curve) quantitative measurement of microbial growth batch culture system continuous culture system generation time Students will be taught: how to calculate the generation time of bacteria; different phases of the bacterial growth curve; and difference between a batch culture system and a continuous culture system. MID-SEMESTER TEST 4

5 10-13 PATHOGENIC BACTERIA AND DISEASES Air borne diseases Food borne diseases Direct contact diseases Arthropod borne diseases Virulence, sources, symptoms, treatment and control of the diseases. Koch s postulates Examples of some common human bacterial diseases of various sources will be discussed and students will know the causative organisms, virulence factors, symptoms, treatment and control of these diseases. Students will be taught the principles of Koch s postulates, and exposed to the practical of isolating and identifying the CAUSATIVE BACTERIUM FOR A PARTICULAR INFECTION. 14 METHODS OF ISOLATION OF BACTERIAL PATHOGENS Streak plate Pour plate Spread plate Students will practically collect samples from human and other sources, and isolate bacteria from such. They will also be expected to obtain PURE ISOLATES from samples. 15 REVISION This is the week preceding the final examination. At this time, evaluation will be done to assess how far the students expectations for the course have been met. 5

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