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1 with the financial support of the French Ministry of Foreign Affairs Biotechnology Platform for Research and Training on Parasitic Zoonoses in South East Asia Workshop / Training on Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory Yogyakarta, May 19 24, 2014 Faculty of Veterinary Medicine Gadjah Mada University

2 Trypanosomes and their vectors Trypanosoma evansi causes a trypanosomosis (Surra), mechanically transmitted by haematophagous flies, such as Tabanids and Stomoxes. The principal host species of T. evansi varies geographically in Indonesia, horses, buffalos and cattle are particularly affected, including wildlife and rodents. Human infections have been recently described in India, surra a potential emerging parasitic zoonosis. Rodents are also a reservoir of Trypanosoma lewisi, that could be found in Indonesia

Trypanosomiasis in Indonesia Thailand Philipines Malaysia Malaysia Sumatra Singapore West Papua Java Kalimantan Sulawesi Yogyakarta West Nusa East Nusa

3 Trypanosomiasis in Indonesia Thailand Philipines Malaysia Malaysia Sumatra Singapore West Papua Java Kalimantan Sulawesi Yogyakarta West Nusa East Nusa Tenggara Tenggara Australia Cattle Trypanosomiasis Buffalo Trypanosomiasis Horse Trypanosomiasis (Cattle also) Cattle and Buffalo Trypanosomiasis BioTraps

Vectors and Pathogens Suborder Family Species Nematocera Culicidae Anopheles sp

Dengue, Arbovirus, etc) Simulium sp (Onchocercosis) Culicoides sp

Glossinidae Glossina sp (Trypanosomiasis) Tabanidae Muscidae Tabanus sp

4 Vectors and Pathogens Suborder Family Species Nematocera Culicidae Anopheles sp (malaria vector) Simuliidae Ceratopogonidae Psychodidae Culex sp (virus vector: Dengue, Arbovirus, etc) Simulium sp (Onchocercosis) Culicoides sp (Leucocytozoonosis, Blue tongue) Phlebotomus sp (Leishmaniasis) Brachycera Glossinidae Glossina sp (Trypanosomiasis) Tabanidae Muscidae Tabanus sp (Trypanosomiasis, Anaplasmosis) Musca sp (bacterial vector) Stomoxys sp (Trypanosomiasis, Dermatophilus)

5 Musca domestica Family: Muscidae (House-flies and Stable-flies) Mechanical transmission of pathogens, accidental myiasis. Stomoxys calcitrans biting pest (human and veterinary pest)

7 Trypanosoma and Surra in Indonesia Many Trypanosomosis outbreaks occur in bovines in Indonesia, and many biting flies as mechanical vector of Trypanosomosis. 28 species of Tabanus, 5 Chrysops species and 5 Haematopota sp. were reported as potential vectors of surra in Indonesia The high density of stable fly populations can severely decrease livestock productivity.

8 Outbreak in Sumba Island Tabanus and Stomoxys flies has a significant impact in horses and cattle trypanosomosis in Sumba Island in East Nusa Tenggara province of Indonesia during a Surra outbreak in (393 cases, 350 dead). In several Islands in Indonesia, Tabanus rubidus can also transmit zoonotic disease such as Antrax. To date, there is no control method available.

9 Trypanosomosis in Horses

10 Good farming system

11 Garbage farming system

12 Traditional farming system

13 Traditional farming system

14 Dairy cattle livestock

15 NZ1 Fly Trap

16 Sticky paper Diagnosis Herbal and fogging control of human and animal Insecticide trypanosomes Fogging

17 Aims of the workshop To share knowledge and experience on trypanosomes and their vectors: diagnosis epidemiology, impact and control of the disease as well as trapping, identification, studies of transmission mechanisms, epidemiological impact as vectors and ways of control. To strengthen animal health systems approach, focusing on national policy, coordinated at different levels of government to increase technical skills. To support the sustainable Indonesia s veterinary services in Surra erradication according to international standards set by the World Organization for Animal Health (OIE). To develope joint research projects amongst French and South East Asian research institutes and universities.

18 One Health Open workshop 19 th May 2014 The workshop will be held under the umbrellas of GREASE and the NAHIAT, in order to strengthen cross-sectorial and multidisciplinary exchanges about trypanosomosis management among professional form different sectors (Animal health, human health, environment, rural development). Lectures and open discussions will be held on future collaborations in the One Health (OH) conceptual framework, on trypanosomes and vectors, including livestock, wildlife, environment (both social and natural), veterinary and human medicine it will be open to a large audience, especially to represent various geographical areas of Indonesia.

19 Registered Workshop 20 th to 24 th May 2014 Limited to 20 participants Presentations: (1) trypanosomes both in humans and animal reservoir in Asia, diagnosis methods and potential control methods, and (2) their transmission mechanisms and vectors, including trapping, identification and control. It will include lectures, field work, practices and laboratory activities

20 Expected results and outputs To overview the problem of trypanosomes in Asia, both in humans and animals including livestock, domestic and wild animal; To implement and interpret specific diagnosis of trypanosomoses in mammals, using parasitological, serological and DNA based methods; To implement trapping and identification of the main genera/species of biting insects acting as mechanical vectors of trypanosomes in livestock; To draft common research project for the control of biting insects within a OH framework To build up research methodology for epidemio-surveillance of trypanosomoses.

21 Time Programme Lecture/Speaker duration Opening remarks from the committee International seminar on Biting Flies and Trypanosomes Dr. Wisnu Nurcahyo (GMU) 5 min T Opening Speech from GMU Prof. Dr. Suratman (Vice Rector Research GMU) Opening Speech from coordinator of BioZoonoSEA Opening Speech from NAHIAT coordinator (CIRAD-IRD) Prof. Sathaporn Jittapalapong (KU) Dr. Marc Desquesnes (CIRAD / KU) Coffee break 15 min 10 min T 10 min T 5 min T Morning Session L 1 : One Heath / EcoHealth conceptual framework Dr. A Binot 50 min T L 2 : Parasitic Zoonoses in Thailand Pr. S Jittapalapong 20 min T L 3 : VRI and zoonoses in Malaysia Dr C Panchadcharam 20 min T L 4 : Atypical Human Dr S Herder 20 min T Trypanosomoses in SEA L 5 : Biting-Insects as mechanical vectors Dr M Desquesnes 20 min T L 6 : Parasitic zoonoses in Dr Wisnu Nurcahyo 20 min T Indonesia Lunch Time (Courtesy of FVM GMU) 60 min

22 L 7 : Surra in the Philippines Dr A Dargantes 15 min T L 8 : Surra in Malaysia Dr C Panchadcharam 15 min T L 9 : Surra in Thailand Pr S Jittapalapong 15 min T L 10: Surra in Indonesia Dr April Wardana 15 min T (Indonesian Veterinary Research Institute) L 11 : Molecular aspects of surra Pr W Artama 15 min T L 12: Economic impact of surra Dr A Dargantes 15 min T Regional aspects of Surra in Indonesia L 13 : Surra in Bali Dr. Masa Tenaya (Bali Diseases Investigation Center) L 14 : Surra in Java Dr. Fajar Sumping Catur Rasa (Yogyakarta Diseases Investigation Center) L 15 : Surra in Sumatra Syamsul Maarif, DVM, MSc (Lampung Diseases investigation Center) L 16 : Surra in Kalimantan Sulaxono Hadi, DVM (Banjarbaru Diseases Investigation center) L 17 : Surra in Sulawesi Bagoes Purma Jaya, DVM, MSc (Makassar Diseases Investigation Center) L 18 : Surra in Sumba Martono Adi Priyatno, DVM (Veterinarian in East Sumba District) Coffee break 15 min L 19 : Surra in Regulation of surra in indonesia Dr. Pujiatmoko (Director of Animal Health) (90 min) 10 min T 10 min T 10 min T 10 min T 10 min T 10 min T 15 min T L 20: GREASE network Dr A Binot 10 min T Open Discussion on One Health Concepts Dr A Binot (CIRAD / GREASE) 25 min T : Biting flies and Trypanosomes Diagnosis Closing and control remarks of human and animal trypanosomes Dr. A Binot 10 min T and their Announcement vectors in Asia: from field to laboratory Dr. Wisnu Nurcahyo 5 min T

23 Day 2 : Workshop/Training - Tuesday, May 20 th, 2014: Biting flies biology & trapping Time Programme Lecture/Speaker Place/Time Remark Lecture 1 : An overview on Dr Marc Desquesnes T 30 min biting insects min Lecture 2 : Biology of Tabanids and stomoxyine flies Dr Marc Desquesnes T Coffee break 15 min min Lecture 4 : Seasonality of tabanids in Thailand Dr Jumnongjit Phasuk Lecture 5 : Seasonality of Dr Jumnongjit Phasuk 30 min stomoxes in Thailand Lunch Time 60 min Lecture 6 : Trapping Dr Marc Desquesnes 75 min technologies Coffee break 15 min hours Field trip 1: Setting up of insect traps in the Field (KP4 GMU) Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Wisnu Nurcahyo Dr Alan Dargantes Diagnosis Back and control to UC Hotel of human and animal trypanosomes T T T P

24 Day 3: Workshop/Training - Wednesday, May 21 th, 2014: Biting flies trapping & identification Time Programme Lecture/Speaker Place/Time Remark hours Field trip 2: Collect of cages from traps in the field KP4 UGM KP4 GMU P Coffee break 15 min min Lecture 7: Identification of biting flies: generalities & Tabanids Dr Marc Desquesnes FVM GMU P Lunch Time 60 min FVM GMU min Lecture 8: Identification of Stomoxys, Hematobia and Musca crassirostris Dr Jumnongjit Phasuk FVM GMU T Coffee break 15 min FVM GMU hours PW1: Identification of biting insects collected in the Dr Marc Desquesnes Dr Jumnongjit Phasuk morning Dr Alan Dargantes FVM GMU P

25 Day 4: Workshop/Training - Thursday, May 22 th, 2014: Biting flies trapping, identification & control; Trypanosomes: biology & identification Time Programme Lecture/Speaker Place Remarks Field trip 3: Setting up of insect traps in the field of dairy cattle Kaliurang, Sleman Kaliurang P Lecture 9 : Control of biting flies Dr Marc Desquesnes T Coffee break 15 min Lecture 10 : Biology, morphology and pathogeny of Trypanosomes Dr Stéphane Herder Lunch Time (lunch box) 60 min FVM GMU Lecture 11 : Parasitological diagnosis of Trypanosomes Dr Marc Desquesnes T T Lecture 12 : Serological diagnosis of Trypanosomes Dr Marc Desquesnes T Coffee break 15 min FVM GMU PW2: Identification of biting insects Dr Marc Desquesnes FVM GMU collected in the morning Dr Jumnongjit Phasuk Dr Alan Dargantes P

26 Day 5 : Workshop/Training - Friday, May 23 th, 2014: Biting flies trapping & identification Trypanosomes: Molecular diagnosis, characterization and control Time Programme Lecture/Speaker Place Remarks Field trip 4: Setting up of insect traps in the field of beef cattle Glagah Beach, Bantul Kaliurang P Lecture 13: Molecular diagnosis of Trypanosomes Dr. Wayan Artama Coffee break 15 min Lecture 14 : Molecular Dr. Stéphane Herder characterization of Trypanosomes FVM GMU FVM GMU Lunch Time 60 min FVM GMU Lecture 15: Control of surra in the Philippines Dr. A Dargantes FVM GMU P Lecture 16: Control of surra in Dr. Marc Desquesnes FVM GMU P Thailand PW3: Identification of biting flies from dairy cattle Kaliurang, Sleman Dr. Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes FVM GMU P Coffee break 15 min FVM GMU PW4: Practical work in the laboratory Dr. Marc Desquesnes for parasitological diagnosis of Dr Stéphane Herder trypanosomes Dr Wayan Artama Dr Wisnu Nurcahyo T

27 Day 6 : Workshop/Training - Saturday, May 23 th, 2014: Biting flies identification Trypanosome molecular diagnosis Time Programme Lecture/Speaker Place Remarks Identification of biting flies from beef cattle Glagah Beach, Bantul Dr. Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes FVM GMU P Lunch Time 60 min FVM GMU PW5: Practical work in the laboratory of Molecular biology for Trypanosomes Dr Stéphane Herder and Prof. Wayan Artama FVM GMU P Conclusions and Closing ceremony Dr. Marc Desquesnes Dr. Joko Prastowo CIRAD/IRD Dean FVM GMU

28 No Name of Institution Origin (Province) 1 Faculty of Veterinary Medicine, UGM Yogyakarta 2 Faculty of Veterinary Medicine, University of Udayana Bali 3 Faculty of Veterinary Medicine, Airlangga University, Surabaya East Java 4 Faculty of Veterinary Medicine, Brawijaya University, Malang East Java 5 Faculty of Veterinary Medicine, Nusa Cendana, Kupang East Nusa Tenggara 6 Faculty of Veterinary Medicine, Syiah Kuala Univ, Banda Aceh Banda Aceh 7 Faculty of Veterinary Medicine, NTB Univ Mataram West Nusa Tenggara 8 Faculty of Veterinary Medicine, Wijayakusuma, Surabaya East Java 9 Veterinary Study Programme, Hassanuddin Univ, Makassar South Sulawesi 10 Bogor Agriculture University, Bogor West Java 11 Veterinary Research Institute, Bogor West Java 12 Disease Investigation Center, Yogyakarta Yogyakarta 13 Disease Investigation Center, Denpasar, Bali Bali 14 Disease Investigation Center, Banjarbaru, Kalimantan Kalimantan 15 Disease Investigation Center, Lampung South Sumatra 16 Disease Investigation Center, Bukittinggi, West Sumatra West Sumatra 17 Disease Investigation Center, Medan North Sumatra 18 Disease Investigation Center, Makassar South Sulawesi 19 Faculty of Biology, UGM Yogyakarta 20 Faculty of Medical, UGM Yogyakarta 21 Vector Research Station, Ministry of Health, Salatiga Central Java 22 Rodents Research Station, Banjarnegara Central Java 23 Zoonoses Division, Ministry of Health, Jakarta Jakarta 24 Directorate of Animal Health, Ministry of Agriculture Jakarta

29 Activities International Seminar Keynote Speakers (CIRAD, GREASE) Invited speakers (Asean Country) Theory and Practical workshop Biting flies Trypanosomes Field trips Bionomics and habitats of vectors Trapping and Field identification

30 Secretariat: Dept of Parasitology Faculty of Veterinary Medicine Gadjah Mada University Jalan Fauna 2, Karangmalang Yogyakarta Website : Phone and fax : Mobile :

31 Workshop Thailand

32 Workshop at NUS Singapore

33 VRI Malaysia

34 City tour Yogyakarta 3/28/2014 Diagnosis and control of human Institute and for animal Research trypanosomes and Community Services, UGM 34

35 See you in Indonesia May 19-25, 2014 Thank you for your attention

BioZoonoSEA. Biotechnology Platform for Research and Training on Parasitic Zoonoses in South East Asia

BioZoonoSEA. Biotechnology Platform for Research and Training on Parasitic Zoonoses in South East Asia BioZoonoSEA Biotechnology Platform for Research and Training on Parasitic Zoonoses in South East Asia with the financial support of the French Ministry of Foreign Affairs SUMMARY REPORT on the Workshop