Method Preferences and Test Accuracy of Antimicrobial Susceptibility Testing
|
|
- Annice Bailey
- 5 years ago
- Views:
Transcription
1 Method Preferences and Test Accuracy of Antimicrobial Susceptibility Testing Updates From the College of American Pathologists Microbiology Surveys Program (2000) Ronald N. Jones, MD; for the College of American Pathologists Microbiology Resource Committee Objective. To summarize the antimicrobial susceptibility testing results from the College of American Pathologists (CAP) Microbiology Surveys Program for Specifically, the frequency of tests used and the quantitative and qualitative (susceptibility category) accuracy were assessed. Design. The CAP Microbiology Surveys challenged subscribers in 2000 with 3 well-characterized organisms for antimicrobial susceptibility testing in pure culture. Each laboratory was to use the test method and reporting procedures routinely applied to patient samples. The strains were National Committee for Clinical Laboratory Standards (NCCLS) quality control organisms with precisely defined antimicrobial susceptibility patterns and reproducibility. Results reported by participants ( /sample) were graded for categorical accuracy and quantitative performance by comparing reported minimal inhibitory concentrations ( g/ml) or zone diameters (mm) against quality control ranges published by the NCCLS. The appropriateness of reported drugs was determined in the context of the type and anatomic location of the infection. Results. The tests most often used varied by the species of the organism and growth characteristics of the isolated strains. Nonfastidious, rapid-growing Surveys unknowns (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) were most often tested with commercial systems (, 42.0% 42.4%; Vitek, 41.5% 43.0%) or with the standardized disk diffusion method (12.8% 13.9%). In contrast, fastidious species, such as Streptococcus pneumoniae (ATCC 49619), were predominantly tested by Etest (40.3%), followed by disk diffusion (27.6%) and (23.2%). Categorical accuracy was essentially equal between dilution (98.9%) and diffusion (99.0%) methods. Among the minimal inhibitory concentration methods used to test penicillin against S pneumoniae, Etest method quantitative accuracy (96.3%) was greater than that of (92.4%). Quantitative accuracy was greatest for dilution minimal inhibitory concentration methods, with more than 90% of results within NCCLS quality control ranges for nearly all reported antimicrobials. Reevaluations of quality control ranges may be needed for 4 to 7 agents, depending on method. Reporting errors were also detected in 2 areas: (1) reporting results for drugs not active at the site of infection and (2) reporting results for drugs tested with suboptimal methods without published NCCLS interpretive criteria. Conclusions. Antimicrobial susceptibility testing methods used in US laboratories were dominated by commercial products with relatively high accuracy (qualitative and quantitative). As available methods have become better suited to both fastidious and rapid-growing species, reporting errors have assumed a higher level of concern to the CAP Surveys in an effort to minimize prescription errors. (Arch Pathol Lab Med. 2001;125: ) The College of American Pathologists (CAP) Surveys Program for clinical microbiology represents one of the largest comprehensive external quality assurance programs in the world. A broad variety of Surveys are available; they include 2 to 3 clinical challenge mailings of specimens covering the topics of bacteriology, mycology, mycobacteriology, parasitology, virology, and associated cell culture diagnostics, as well as focused programs on molecular diagnostics and epidemiology of infectious diseases. An important component of the CAP Bacteriology Accepted for publication June 1, From Tufts University School of Medicine, Boston, Mass, and The JONES Group/JMI Laboratories, North Liberty, Iowa. Reprints: Ronald N. Jones, MD, 345 Beaver Kreek Centre, Suite A, North Liberty, IA ( ronald-jones@jonesgr.com). Survey has been the antimicrobial susceptibility testing challenges (3 4 organisms/y). Previous reports from this program have documented consistent high-quality performance overall, 1 11 but periodic methods or commercial product deficiencies have led to technical or methods modifications by the National Committee for Clinical Laboratory Standards (NCCLS) and/or product changes by various commercial systems. The results of the 2000 CAP Surveys are summarized in this article, including the tabulation of the most utilized antimicrobial susceptibility testing methods or systems. MATERIALS AND METHODS In 2000, the CAP Microbiology Surveys (D-series) had 3857 subscribing laboratories that were sent 3 unknown challenge organisms for routine susceptibility testing. The strains included Arch Pathol Lab Med Vol 125, October 2001 Antimicrobial Susceptibility Testing Jones 1285
2 Table 1. Use Statistics From the College of American Pathologists Surveys Program for 2000 Listing Antimicrobial Susceptibility Test Methods and Commercial Systems* No. of Occurrences (%) for Each Sample D-03 D-09 Method or System (Streptococcus pneumoniae) (Escherichia coli) Disk diffusion 740 (27.6) 351 (12.8) 413 (13.9) Dilution methods Agar Broth (macro) Broth (micro) Commercial API Micromedia PASCO Sceptor Sensititre Vitek Etest Total No. of responses * indicates not tabulated. Includes 5 Vitek-II users (72.4) 622 (23.2) 16 (0.6) 1083 (40.3) (87.2) 6 (0.2) 11 (0.4) 1( 0.1) 3 (0.1) 1151 (42.0) 16 (0.6) 4 (0.2) 10 (0.4) 1179 (43.0) 2( 0.1) 2739 D-17 (Pseudomonas aeruginosa) 2566 (86.1) 6 (0.2) 12 (0.4) 10 (0.3) 1( 0.1) 1261 (42.4) 16 (0.5) 4 (0.1) 13 (0.4) 1228 (41.5) 2979 well-characterized quality control (QC) strains recommended for regular, routine use by the NCCLS These strains were Escherichia coli (ATCC 25922; sample D-09, 2000), Pseudomonas aeruginosa (ATCC 27853; sample D-17, 2000), and Streptococcus pneumoniae (ATCC 49619; sample D-03, 2000). Each strain was to be processed by identification and susceptibility methods routinely used in the participating laboratory, with the reporting of only those antimicrobial agents considered appropriate for the clinical settings stated on the CAP Survey. The clinical infection settings for each pathogen were meningitis caused by S pneumoniae, blood stream infection caused by E coli, and a P aeruginosa lower respiratory tract infection diagnosed by a simulated bronchoalveolar lavage. The reporting of antimicrobials that achieved clinically adequate concentrations only in the urine (cinoxacin, nitrofurantoin, norfloxacin, trimethoprim, etc) was considered unacceptable performance for these 3 samples. Acceptable graded categorical results were those achieved by greater than 90% of both referees and participants. RESULTS AND COMME Table 1 lists the methods and commercial systems utilized by the CAP Surveys participants in processing the year 2000, Survey D organisms. A total of 2685 to 2979 (69.9% 77.6% of all Surveys participants) sets of test results were submitted by the subscribers. Very consistent methods results were noted for challenges D-09 and D-17, for which nearly 12.8% to 13.9% of laboratories still used the standardized disk diffusion method. 12 Among the dilution (manual or automated) tests or systems reported, the 2 dominant systems were Vitek (41.5% 43.0%) and (42.0% 42.4%). Use of any other single method or system by participants was less than 1.0%. In contrast (Table 1), the Etest (AB BIODISK, Solna, Sweden) was the principal method (40.3%) used for testing the fastidious organism, S pneumoniae. The other most commonly used methods to test pneumococci were the disk diffusion test (27.6%), (23.2%), and PAS- CO (0.6%). Other methods were infrequently used, usually because of organism-specific method/system disclaimers, and were not included to tabulate rates. Table 2 lists the susceptibility testing categorical accuracy (susceptible, intermediate, resistant) for specimens D- 09 and D-17. High-level accuracy was demonstrated for all listed agents for disk diffusion (90.0% %; average 98.96%) and minimal inhibitory concentration (MIC) (90.2% %; average 98.92%) test users. These rates compare favorably to prior Surveys reports, before the 90% participant and referee consensus grading policy was instituted. The standard for grading of the earlier Surveys challenges was greater than 80% consensus of results from selected referees who used NCCLS methods, 12,13 not commercial or automated systems. The evaluation of the S pneumoniae isolate (clinical setting of meningitis) produced highly accurate categorical values from the 3 most frequently used MIC methods (Table 3). The quantitative accuracy of Etest and methods was also very acceptable when compared with published NCCLS QC ranges (Table 4). Among the appropriate antimicrobial agents listed in Table 3 (cefotaxime, ceftriaxone, chloramphenicol, penicillin, and vancomycin), the test accuracy was 98.0%, 98.2%, and 97.1% for, Etest, and PASCO, respectively. For penicillin tests, the test accuracy was slightly less, and the accuracy rank order for these products was Etest (94.1%), followed by (91.3%) and PASCO (90.6%). This degree of interpretive (categories) and quantitative accuracy for the evaluated dilution test systems was remarkably high and unprecedented in the CAP Surveys using a S pneumoniae challenge strain. 1,5,6,9 For the pneumococcal Surveys sample, several laboratories reported disk diffusion results for antimicrobials for which no current interpretive criteria are available in NCCLS tables and/or the disk diffusion method was considered suboptimal. 12 These results were graded as unacceptable, regardless of their agreement with consensus qualitative, categorical susceptibility values. Similarly, many dilution test users reported antimicrobial agents that did not achieve adequate therapeutic concentrations in cerebrospinal fluid. This routine practice of reporting clinically inappropriate antimicrobial agents results could cause some physicians to select useless therapeutic agents. Selection of such drugs could lead to adverse or fatal clinical outcomes in this clinically emergent setting (cerebrospinal fluid and meningitis). These results were not penalized, but subsequent participant reports of test results of this type will result in unsatisfactory grades Arch Pathol Lab Med Vol 125, October 2001 Antimicrobial Susceptibility Testing Jones
3 Table 2. Categorical Accuracy 14,16 of Antimicrobial Susceptibility Testing Methods for 2 College of American Pathologists Survey Challenge Strains in 2000 (D-09, Escherichia coli; D-17, Pseudomonas aeruginosa) Antimicrobial Amikacin Amoxicillin/clavulanate Ampicillin Ampicillin/Sulbactam Aztreonam Carbenicillin Cefazolin Cefepime Cefoperazone Cefotaxime Cefotetan Cefoxitin Ceftazidime Ceftizoxime Ceftriaxone Cefuroxime Cephalothin Ciprofloxacin Gentamicin Imipenem Levofloxacin Meropenem Mezlocillin Ofloxacin Piperacillin Piperacillin/tazobactam Tetracycline Ticarcillin Ticarcillin/clavulanate Tobramycin Trimethoprim/sulfamethoxazole Disk Diffusion D-09 D Minimal Inhibitory Concentration Methods D-09 D The NCCLS Subcommittee on Antimicrobial Susceptibility Testing will address infection site specific guidelines (meningitis, urine, other sites) for future publication. 14 The position statement of the CAP Microbiology Resource Committee and more detailed survey critiques for each of these specimens cited can be found at the CAP Web site ( Use of NCCLS QC strains as Surveys specimens affords the opportunity to judge participant performance on qualitative and quantitative scales. Defined QC ranges of MICs or millimeter zones 14 can be compared with the participant-reported quantitative results. Table 5 lists the proportions of participant results found within published NCCLS QC ranges 14 for a subset of problematic antimicrobials. Based on these contemporary real-world statistics from a large volume of CAP Survey participants, Table 3. Categorical Accuracy of the 3 Most Commonly Reported Dilution Test Systems/Methods for the 10 Most Frequently Tested Antimicrobial Agents (8018 responses; Streptococcus pneumoniae ATCC used in College of American Pathologists Specimen D-03, 2000) Antimicrobial Agent (No. of Tests)* Appropriate agents Cefotaxime (1023) Ceftriaxone (1257) Chloramphenicol (517) Penicillin (1807) Vancomycin (1024) Inappropriate agents Clindamycin (431) Erythromycin (699) Levofloxacin (187) Tetracycline (472) Trimethoprim/sulfamethoxazole (527) % Categorical Accuracy (No. of Tests) (4223) Etest (3570) PASCO (285) * Number of tests for the 3 listed systems/methods. The categorical accuracy 16 was acceptable; however, reporting of these agents for a case of meningitis would be considered inappropriate. Arch Pathol Lab Med Vol 125, October 2001 Antimicrobial Susceptibility Testing Jones
4 Table 4. Quantitative Accuracy* of Commonly Used Dilution Test Systems/Methods When Testing 10 Antimicrobials Against Streptococcus pneumoniae ATCC (College of American Pathologists Specimen D-03, 2000) Antimicrobial Agent Appropriate agents Cefotaxime Ceftriaxone Chloramphenicol Penicillin Vancomycin Inappropriate agents Erythromycin Clindamycin Levofloxacin Tetracycline Trimethoprim/sulfamethoxazole 96.4 (502) (552) 96.7 (421) 92.4 (622) 93.9 (587) (477) (376) 45.4 (412) 94.5 (439) % Accuracy (No. of Tests) Etest 88.9 (442) 92.3 (552) 92.4 (66) 96.3 (1083) 90.6 (360) 99.5 (195) 98.9 (174) 60.8 (51) Total (All Methods) 93.3 (1052) 95.3 (1319) 96.3 (563) (1875) 92.9 (1074) 97.9 (766) 96.6 (468) 99.0 (199) 53.4 (521) 90.7 (578) * Accuracy determined by the number of reported minimal inhibitory concentration (MIC) values within published MIC quality control ranges 16 or manufacturer s package insert (AB BIODISK, Solna, Sweden). Only those organism/system or method combinations with 50 reported results for the tested drug are listed. Drugs considered inappropriate for reporting in a clinical case of meningitis. several antimicrobials may require reevaluation of their QC ranges. Examples of the most aberrant results for the disk diffusion test were as follows (percentage of results within control limits; target 90%): Ecoli, ticarcillin/clavulanate (54.0%), cefazolin (61.7%), and amoxicillin/clavulanate (70.0%); P aeruginosa, ofloxacin (72.7%) and ticarcillin (71.6%). The antimicrobials needing further study of MIC QC ranges were more limited (percentage of results within control guidelines): P aeruginosa, amikacin (80.3%) and cefotaxime (82.5%; data not shown). These results from the antimicrobial susceptibility testing component of the 2000 CAP Microbiology Surveys demonstrate acceptable test accuracy and reproducibility overall for the most commonly used testing systems or methods. Few diagnostic problems were identified, but some possible QC guideline flaws need rapid resolution. To minimize potentially fatal prescription errors (1999 Report of the Institute of Medicine, Washington, DC), 15 clinical laboratories performing susceptibility tests must carefully select agents for reporting in emergent clinical infections (meningitis, bacteremia). 16 Where antimicrobial susceptibility testing results are reported for epidemiologic purposes only, in addition to potential therapeutic agents for meningitis, these results should be clearly delineated as not having relevance to the treatment of the index case. No laboratory should have complicity in the negligence of placing a patient in harm s way by reporting test results leading to inappropriate therapeutic choices by clinicians. The CAP Microbiology Resource Committee appreciates the constructive suggestions received from Surveys participants. Participant input will serve to strengthen the program and elevate the quality of our laboratory discipline. Members of the College of American Pathologists Microbiology Resource Committee included W. C. Winn, Jr, chair (University of Vermont College of Medicine, Burlington, Vt); J. C. Steele, Jr, vice-chair (Medical College of Georgia, Augusta, Ga); A. D. Alrahwan (University of Texas, San Antonio, Tex); K. Beavis (Cook County Hospital, Chicago, Ill); J. D. Christie (East Carolina University, Greenville, NC); J. L. Harris (Pathology Associates, Tallahassee, Fla); E. Koneman (Breckenridge, Colo); D. N. Persing (Corixa Corp, Seattle, Wash); M. A. Pfaller (University of Iowa, Iowa City); W. O. Rogers (NMRC Malaria Program, Silver Springs, Md); J. Versalovic (Massachusetts General Hospital, Boston); M. M. Yungbluth (St Francis Hospital, Evanston, Ill); D. L. Church, Consultant (Calgary Laboratory Services, Calgary, Alberta); and L. S. Garcia, consultant (Santa Monica, Calif). Table 5. Quantitative Performance of Disk Diffusion Test Users in the College of American Pathologists Surveys (D-09 and D-17, 2000). Only Those Agents With Suboptimal Quantitative Performance Are Listed or 80% of Participant Results Within National Committee for Clinical Laboratory Standards Quality Control Guidelines 16 Antimicrobial Agent (No. of Responses) Amoxicillin/clavulanate (70) Ampicillin/sulbactam (88) Cefazolin (219) Cefepime (35) Cefoperazone (30) Cefotaxime (54) Ceftriaxone (164) Ofloxacin (22) Ticarcillin (53) Ticarcillin/clavulanate (50) Trimethoprim/sulfamethoxazole (133) D-09 (Escherichia coli ATCC 25922) NCCLS Range, mm Participant Results Mean Zone, mm % in Range D-17 (Pseudomonas aeruginosa ATCC 27853) NCCLS Range, mm Participant Results Mean Zone, mm % in Range 1288 Arch Pathol Lab Med Vol 125, October 2001 Antimicrobial Susceptibility Testing Jones
5 The coauthors thank the following support staff at the College of American Pathologists for significant contributions to the 2000 Surveys Program and analyses of participant data: D. Bird and S. Schneider. Additional appreciation is extended to K. L. Meyer for manuscript suggestions and preparation. References 1. Doern GV, Brueggemann AB, Pfaller MA, Jones RN. Assessment of laboratory performance with Streptococcus pneumoniae antimicrobial susceptibility testing in the United States: a report from the College of American Pathologists Microbiology Proficiency Survey Program. Arch Pathol Lab Med. 1999;123: Jones RN. Antimicrobial susceptibility testing (AST): a review of changing trends, quality control guidelines, test accuracy, and recommendation for the testing of -lactam drugs. Diagn Microbiol Infect Dis. 1983;1: Jones RN. Recent trends in the College of American Pathologists proficiency results for antimicrobial susceptibility testing: preparing for CLIA 88. Clin Microbiol Newsl. 1992;14: Jones RN, Edson DC. Interlaboratory performance of disk agar diffusion and dilution antimicrobial susceptibility tests, : a summary of the microbiology portion of the College of American Pathologists (CAP) Surveys. Am J Clin Pathol. 1982;78(suppl): Jones RN, Edson DC. The ability of participant laboratories to detect penicillin-resistant pneumococci: a report from the microbiology portion of the College of American Pathologists (CAP) Surveys. Am J Clin Pathol. 1982;78(suppl): Jones RN, Edson DC. Special topics in antimicrobial susceptibility testing: test accuracy against methicillin-resistant Staphylococcus aureus, pneumococci, and the sensitivity of -lactamase methods. Am J Clin Pathol. 1983;80(suppl): Jones RN, Edson DC. Antibiotic susceptibility testing accuracy: a review of the College of American Pathologists (CAP) Microbiology Survey, Arch Pathol Lab Med. 1985;109: Jones RN, Edson DC, for the Microbiology Resource Committee of the College of American Pathologists. The identification and antimicrobial susceptibility testing of Neisseria gonorrhoeae, : results from the College of American Pathologists Microbiology Surveys Program. Arch Pathol Lab Med. 1988; 112: Jones RN, Edson DC. Antimicrobial susceptibility testing (AST) trends and accuracy in the United States: a review of the College of American Pathologists Microbiology Surveys, Arch Pathol Lab Med. 1991;115: Jones RN, Edson DC, Gilmore BF. Contemporary quality control practices for antimicrobial susceptibility tests: a report from the microbiology portion of the College of American Pathologists (CAP) Surveys Program. Am J Clin Pathol. 1983;80(suppl): Jones RN, Edson DC, Marymont JV. Evaluations of antimicrobial susceptibility test proficiency by the College of American Pathologists Survey Program: a clarification of quality control recommendations. Am J Clin Pathol. 1982;78: National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests. 7th ed. Approved standard M2- A7. Wayne, Pa: National Committee for Clinical Laboratory Standards; National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 5th ed. Approved standard M7-A5. Wayne, Pa: National Committee for Clinical Laboratory Standards; National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Susceptibility Testing. 10th informational supplement M100-S10. Wayne, Pa: National Committee for Clinical Laboratory Standards; Barlas S. The push to report medical errors. Pharm Ther. 2001;26: Gilbert DN, Moellering RC, Sande MA. The Sanford Guide to Antimicrobial Therapy, th ed. Dallas, Tex: Antimicrobial Therapy Inc; Arch Pathol Lab Med Vol 125, October 2001 Antimicrobial Susceptibility Testing Jones 1289
a. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2.
AND QUANTITATIVE PRECISION (SAMPLE UR-01, 2017) Background and Plan of Analysis Sample UR-01 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony
More informationBackground and Plan of Analysis
ENTEROCOCCI Background and Plan of Analysis UR-11 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony count, to perform the identification
More informationCAP Laboratory Improvement Programs. Performance Accuracy of Antibacterial and Antifungal Susceptibility Test Methods
CAP Laboratory Improvement Programs Performance Accuracy of Antibacterial and Antifungal usceptibility Test Methods Report From the College of American Pathologists Microbiology urveys Program (001 003)
More informationEUCAST recommended strains for internal quality control
EUCAST recommended strains for internal quality control Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae ATCC 59 ATCC
More informationPerformance Information. Vet use only
Performance Information Vet use only Performance of plates read manually was measured in three sites. Each centre tested Enterobacteriaceae, streptococci, staphylococci and pseudomonas-like organisms.
More informationEuropean Committee on Antimicrobial Susceptibility Testing
European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control as recommended by EUCAST Version 5.0, valid from 015-01-09 This document should be cited as "The
More informationWhat s new in EUCAST methods?
What s new in EUCAST methods? Derek Brown EUCAST Scientific Secretary Interactive question 1 MIC determination MH-F broth for broth microdilution testing of fastidious microorganisms Gradient MIC tests
More informationJanuary 2014 Vol. 34 No. 1
January 2014 Vol. 34 No. 1. and Minimum Inhibitory Concentration (MIC) Interpretive Standards for Testing Conditions Medium: diffusion: Mueller-Hinton agar (MHA) Broth dilution: cation-adjusted Mueller-Hinton
More informationAntimicrobial Stewardship Strategy: Antibiograms
Antimicrobial Stewardship Strategy: Antibiograms A summary of the cumulative susceptibility of bacterial isolates to formulary antibiotics in a given institution or region. Its main functions are to guide
More informationConcise Antibiogram Toolkit Background
Background This toolkit is designed to guide nursing homes in creating their own antibiograms, an important tool for guiding empiric antimicrobial therapy. Information about antibiograms and instructions
More informationUnderstanding the Hospital Antibiogram
Understanding the Hospital Antibiogram Sharon Erdman, PharmD Clinical Professor Purdue University College of Pharmacy Infectious Diseases Clinical Pharmacist Eskenazi Health 5 Understanding the Hospital
More informationAntimicrobial Susceptibility Testing: Advanced Course
Antimicrobial Susceptibility Testing: Advanced Course Cascade Reporting Cascade Reporting I. Selecting Antimicrobial Agents for Testing and Reporting Selection of the most appropriate antimicrobials to
More informationAntimicrobial Susceptibility Testing: The Basics
Antimicrobial Susceptibility Testing: The Basics Susan E. Sharp, Ph.D., DABMM, FAAM Director, Airport Way Regional Laboratory Director, Regional Microbiology and Molecular Infectious Diseases Laboratories
More informationThe Basics: Using CLSI Antimicrobial Susceptibility Testing Standards
The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards Janet A. Hindler, MCLS, MT(ASCP) UCLA Health System Los Angeles, California, USA jhindler@ucla.edu 1 Learning Objectives Describe information
More informationRoutine internal quality control as recommended by EUCAST Version 3.1, valid from
Routine internal quality control as recommended by EUCAST Version.1, valid from 01-01-01 Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus
More informationEvaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals
J Vet Diagn Invest :164 168 (1998) Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals Susannah K. Hubert, Phouc Dinh Nguyen, Robert D. Walker Abstract.
More informationEuropean Committee on Antimicrobial Susceptibility Testing
European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST Version 8.0, valid from 018-01-01
More informationEducating Clinical and Public Health Laboratories About Antimicrobial Resistance Challenges
Educating Clinical and Public Health Laboratories About Antimicrobial Resistance Challenges Janet Hindler, MCLS MT(ASCP) UCLA Medical Center jhindler@ucla.edu also working as a consultant with the Association
More informationCompliance of manufacturers of AST materials and devices with EUCAST guidelines
Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The
More informationJanuary 2014 Vol. 34 No. 1
January 2014 Vol. 34 No. 1. and Minimal Inhibitory Concentration (MIC) Interpretive Standards for Testing Conditions Medium: diffusion: Mueller-Hinton agar (MHA) roth dilution: cation-adjusted Mueller-Hinton
More informationHelp with moving disc diffusion methods from BSAC to EUCAST. Media BSAC EUCAST
Help with moving disc diffusion methods from BSAC to EUCAST This document sets out the main differences between the BSAC and EUCAST disc diffusion methods with specific emphasis on preparation prior to
More informationSuggestions for appropriate agents to include in routine antimicrobial susceptibility testing
Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing These suggestions are intended to indicate minimum sets of agents to test routinely in a diagnostic laboratory
More informationEvaluation of the BIOGRAM Antimicrobial Susceptibility Test System
JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1985, p. 793-798 0095-1137/85/110793-06$02.00/0 Copyright 1985, American Society for Microbiology Vol. 22, No. 5 Evaluation of the BIOGRAM Antimicrobial Susceptibility
More information2016 Antibiotic Susceptibility Report
Fairview Northland Medical Center and Elk River, Milaca, Princeton and Zimmerman Clinics 2016 Antibiotic Susceptibility Report GRAM-NEGATIVE ORGANISMS 2016 Gram-Negative Non-Urine The number of isolates
More informationAntimicrobial Susceptibility Patterns
Antimicrobial Susceptibility Patterns KNH SURGERY Department Masika M.M. Department of Medical Microbiology, UoN Medicines & Therapeutics Committee, KNH Outline Methodology Overall KNH data Surgery department
More informationجداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی
جداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی ویرایش دوم بر اساس ed., 2017 CLSI M100 27 th تابستان ۶۹۳۱ تهیه
More information2 0 hr. 2 hr. 4 hr. 8 hr. 10 hr. 12 hr.14 hr. 16 hr. 18 hr. 20 hr. 22 hr. 24 hr. (time)
Key words I μ μ μ μ μ μ μ μ μ μ μ μ μ μ II Fig. 1. Microdilution plate. The dilution step of the antimicrobial agent is prepared in the -well microplate. Serial twofold dilution were prepared according
More information2015 Antibiotic Susceptibility Report
Citrobacter freundii Enterobacter aerogenes Enterobacter cloacae Escherichia coli Haemophilus influenzenza Klebsiella oxytoca Klebsiella pneumoniae Proteus mirabilis Pseudomonas aeruginosa Serratia marcescens
More informationTHE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS
THE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS Stefanie Desmet University Hospitals Leuven Laboratory medicine microbiology stefanie.desmet@uzleuven.be
More informationShort Report. R Boot. Keywords: Bacteria, antimicrobial susceptibility testing, quality, diagnostic laboratories, proficiency testing
Short Report Frequent major errors in antimicrobial susceptibility testing of bacterial strains distributed under the Deutsches Krebsforschungszentrum Quality Assurance Program R Boot Former Section of
More informationCompliance of manufacturers of AST materials and devices with EUCAST guidelines
Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The
More informationMark Your Calendars Now! Next Event Ships: September 14, 2015
www.wslhpt.org 2601 Agriculture Drive Madison, WI 53718 (800) 462-5261 (608) 265-1111 Shipment Date: June 15, 2015 Questions or comments should be directed to Amanda Weiss at 800-462-5261 x51 or amanda.weiss@slh.wisc.edu.
More informationJasmine M. Chaitram, 1,2 * Laura A. Jevitt, 1,2 Sara Lary, 1,2 Fred C. Tenover, 1,2 and The WHO Antimicrobial Resistance Group 3,4
JOURNAL OF CLINICAL MICROBIOLOGY, June 2003, p. 2372 2377 Vol. 41, No. 6 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.6.2372 2377.2003 The World Health Organization s External Quality Assurance System Proficiency
More informationOriginal Article. Ratri Hortiwakul, M.Sc.*, Pantip Chayakul, M.D.*, Natnicha Ingviya, B.Sc.**
Original Article In Vitro Activity of Cefminox and Other β-lactam Antibiotics Against Clinical Isolates of Extended- Spectrum-β-lactamase-Producing Klebsiella pneumoniae and Escherichia coli Ratri Hortiwakul,
More informationQUICK REFERENCE. Pseudomonas aeruginosa. (Pseudomonas sp. Xantomonas maltophilia, Acinetobacter sp. & Flavomonas sp.)
Pseudomonas aeruginosa (Pseudomonas sp. Xantomonas maltophilia, Acinetobacter sp. & Flavomonas sp.) Description: Greenish gray colonies with some beta-hemolysis around each colony on blood agar (BAP),
More informationINFECTIOUS DISEASES DIAGNOSTIC LABORATORY NEWSLETTER
INFECTIOUS DISEASES DIAGNOSTIC LABORATORY NEWSLETTER University of Minnesota Health University of Minnesota Medical Center University of Minnesota Masonic Children s Hospital May 2017 Printed herein are
More informationComparison of antibiotic susceptibility results obtained with Adatab* and disc methods
J Clin Pathol 1984;37:159-165 Comparison of antibiotic susceptibility results obtained with Adatab* and disc methods JJS SNELL, MVS DANVERS, PS GARDNER From the Division of Microbiological Reagents and
More informationChildrens Hospital Antibiogram for 2012 (Based on data from 2011)
Childrens Hospital Antibiogram for 2012 (Based on data from 2011) Prepared by: Department of Clinical Microbiology, Health Sciences Centre For further information contact: Andrew Walkty, MD, FRCPC Medical
More informationmicrobiology testing services
microbiology testing services You already know Spectra Laboratories for a wide array of dialysis-related testing services. Now get to know us for your microbiology needs. As the leading provider of renal-specific
More informationBrief reports. Heat stability of the antimicrobial activity of sixty-two antibacterial agents
Journal of Antimicrobial Chemotherapy (5) 35, -5 Brief reports Heat stability of the antimicrobial activity of sixty-two antibacterial agents Walter H. Traub and Birgit Leonhard Institut fur Medizinische
More informationDetection and Quantitation of the Etiologic Agents of Ventilator Associated Pneumonia in Endotracheal Tube Aspirates From Patients in Iran
Letter to the Editor Detection and Quantitation of the Etiologic Agents of Ventilator Associated Pneumonia in Endotracheal Tube Aspirates From Patients in Iran Mohammad Rahbar, PhD; Massoud Hajia, PhD
More informationTel: Fax:
CONCISE COMMUNICATION Bactericidal activity and synergy studies of BAL,a novel pyrrolidinone--ylidenemethyl cephem,tested against streptococci, enterococci and methicillin-resistant staphylococci L. M.
More informationAntimicrobial Pharmacodynamics
Antimicrobial Pharmacodynamics November 28, 2007 George P. Allen, Pharm.D. Assistant Professor, Pharmacy Practice OSU College of Pharmacy at OHSU Objectives Become familiar with PD parameters what they
More information56 Clinical and Laboratory Standards Institute. All rights reserved.
Table 2C 56 Clinical and Laboratory Standards Institute. All rights reserved. Table 2C. Zone Diameter and Minimal Inhibitory Concentration Breakpoints for Testing Conditions Medium: Inoculum: diffusion:
More informationBarriers to Intravenous Penicillin Use for Treatment of Nonmeningitis
JCM Accepts, published online ahead of print on 7 July 2010 J. Clin. Microbiol. doi:10.1128/jcm.01012-10 Copyright 2010, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More information2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine
2012 ANTIBIOGRAM Central Zone Former DTHR Sites Department of Pathology and Laboratory Medicine Medically Relevant Pathogens Based on Gram Morphology Gram-negative Bacilli Lactose Fermenters Non-lactose
More informationBactiReg3 Event Notes Module Page(s) 4-9 (TUL) Page 1 of 21
www.wslhpt.org 2601 Agriculture Drive Madison, WI 53718 (800) 462-5261 (608) 265-1111 2015-BactiR Reg3 Shipment Date: September 14, 2015 Questions or comments should be directed to Amanda Weiss at 800-462-5261
More informationIntrinsic, implied and default resistance
Appendix A Intrinsic, implied and default resistance Magiorakos et al. [1] and CLSI [2] are our primary sources of information on intrinsic resistance. Sanford et al. [3] and Gilbert et al. [4] have been
More informationAnnual Report: Table 1. Antimicrobial Susceptibility Results for 2,488 Isolates of S. pneumoniae Collected Nationally, 2005 MIC (µg/ml)
Streptococcus pneumoniae Annual Report: 5 In 5, a total of, isolates of pneumococci were collected from 59 clinical microbiology laboratories across Canada. Of these, 733 (9.5%) were isolated from blood
More informationAntibiotic. Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting
Antibiotic Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting Any substance of natural, synthetic or semisynthetic origin which at low concentrations kills or inhibits the growth of bacteria
More informationRecommendations to take it forward!
Capacity Building and Strengthening of Hospital Infection Control to detect and prevent antimicrobial resistance in India AIIMS-ICMR-CDC EQAS Recommendations to take it forward! Top regional diagnostic
More informationLab Exercise: Antibiotics- Evaluation using Kirby Bauer method.
Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. OBJECTIVES 1. Compare the antimicrobial capabilities of different antibiotics. 2. Compare effectiveness of with different types of bacteria.
More informationCONTAGIOUS COMMENTS Department of Epidemiology
VOLUME XXIX NUMBER 3 November 2014 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell SM MLS (ASCP), Marti Roe SM MLS (ASCP), Sarah Parker MD, Jason Child PharmD, and Samuel R.
More informationAberdeen Hospital. Antibiotic Susceptibility Patterns For Commonly Isolated Organisms For 2015
Aberdeen Hospital Antibiotic Susceptibility Patterns For Commonly Isolated s For 2015 Services Laboratory Microbiology Department Aberdeen Hospital Nova Scotia Health Authority 835 East River Road New
More informationCONTAGIOUS COMMENTS Department of Epidemiology
VOLUME XXVII NUMBER 6 July 2012 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine B. Dowell SM, MLS (ASCP); Sarah K. Parker, MD; James K. Todd, MD Each year the Children s Hospital Colorado
More informationBacterial Pathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern from a Teaching Hospital, Bengaluru, India
ISSN: 2319-7706 Volume 4 Number 11 (2015) pp. 731-736 http://www.ijcmas.com Original Research Article Bacterial Pathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern from a Teaching
More informationGENERAL NOTES: 2016 site of infection type of organism location of the patient
GENERAL NOTES: This is a summary of the antibiotic sensitivity profile of clinical isolates recovered at AIIMS Bhopal Hospital during the year 2016. However, for organisms in which < 30 isolates were recovered
More informationQuality assurance of antimicrobial susceptibility testing
Quality assurance of antimicrobial susceptibility testing Derek Brown Routine quality control Repeated testing of controls in parallel with tests to ensure that the test system is performing reproducibly
More informationMain objectives of the EURL EQAS s
EQAS Enterococci, Staphylococci and E. coli EURL workshop, April, 11 Lourdes García Migura Main objectives of the EURL EQAS s To improve the comparability of antimicrobial susceptibility testing (AST)
More informationNational Clinical Guideline Centre Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults
National Clinical Guideline Centre Antibiotic classifications Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults Clinical guideline 191 Appendix N 3 December 2014
More informationESBL Producers An Increasing Problem: An Overview Of An Underrated Threat
ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat Hicham Ezzat Professor of Microbiology and Immunology Cairo University Introduction 1 Since the 1980s there have been dramatic
More information21 st Expert Committee on Selection and Use of Essential Medicines Peer Review Report Antibiotics Review
(1) Have all important studies/evidence of which you are aware been included in the application? Yes No Please provide brief comments on any relevant studies that have not been included: (2) For each of
More informationInteractive session: adapting to antibiogram. Thong Phe Heng Vengchhun Felix Leclerc Erika Vlieghe
Interactive session: adapting to antibiogram Thong Phe Heng Vengchhun Felix Leclerc Erika Vlieghe Case 1 63 y old woman Dx: urosepsis? After 2 d: intermediate result: Gram-negative bacilli Empiric antibiotic
More information1. The preferred treatment option for an initial UTI episode in a 22-year-old female patient
1 Chapter 79, Self-Assessment Questions 1. The preferred treatment option for an initial UTI episode in a 22-year-old female patient with normal renal function is: A. Trimethoprim-sulfamethoxazole B. Cefuroxime
More informationAbility of laboratories to detect emerging antimicrobial resistance in nosocomial pathogens: a survey of Project ICARE laboratories
Diagnostic Microbiology and Infectious Disease 38 (2000) 59 67 Surveillance www.elsevier.com/locate/diagmicrobio Ability of laboratories to detect emerging antimicrobial resistance in nosocomial pathogens:
More informationESCMID Online Lecture Library. by author
Quality Assurance of antimicrobial susceptibility testing Derek Brown EUCAST Scientific Secretary ESCMID Postgraduate Education Course, Linz, 17 September 2014 Quality Assurance The total process by which
More informationEXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING
EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING CHN61: EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING 1.1 Introduction A common mechanism of bacterial resistance to beta-lactam antibiotics is the production
More informationOriginal Article. Hossein Khalili a*, Rasool Soltani b, Sorrosh Negahban c, Alireza Abdollahi d and Keirollah Gholami e.
Iranian Journal of Pharmaceutical Research (22), (2): 559-563 Received: January 2 Accepted: June 2 Copyright 22 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services
More informationAPPENDIX III - DOUBLE DISK TEST FOR ESBL
Policy # MI\ANTI\04\03\v03 Page 1 of 5 Section: Antimicrobial Susceptibility Testing Manual Subject Title: Appendix III - Double Disk Test for ESBL Issued by: LABORATORY MANAGER Original Date: January
More informationMercy Medical Center Des Moines, Iowa Department of Pathology. Microbiology Department Antibiotic Susceptibility January December 2016
Mercy Medical Center Des Moines, Iowa Department of Pathology Microbiology Department Antibiotic Susceptibility January December 2016 These statistics are intended solely as a GUIDE to choosing appropriate
More informationANTIMICROBIAL SUSCEPTIBILITY DETECTION OF ELEVATED MICs TO PENICILLINS IN β- HAEMOLYTIC STREPTOCOCCI
HAEMOLYTIC STREPTOCOCCI This specimen was designated as a sample from a skin wound that was to be cultured, identified to species level and susceptibility tested [1-3]. The culture contained a Streptococcus
More informationPrevalence of Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa and its antibiogram in a tertiary care centre
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 952-956 http://www.ijcmas.com Original Research Article Prevalence of Metallo-Beta-Lactamase
More informationDefining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate Confirmation Testing
Infect Dis Ther (2015) 4:513 518 DOI 10.1007/s40121-015-0094-6 BRIEF REPORT Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate
More informationCONTAGIOUS COMMENTS Department of Epidemiology
VOLUME XXIII NUMBER 1 July 2008 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell, SM (ASCP), Marti Roe SM (ASCP), Ann-Christine Nyquist MD, MSPH Are the bugs winning? The 2007
More informationThere are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility
ANTIMICROBIAL SUSCEPTIBILITY TESTING ON MILK SAMPLES Method and guidelines There are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility
More informationREVOLUTIONARY. MMinimum. BBiofilm EEradication Concentration. inimizing WE HAVE FOUND THE ANSWER.
REVOLUTIONARY. Are recurrent bacterial infections a frustration in your practice? WE HAVE FOUND THE ANSWER. MMinimum inimizing BBiofilm EEradication C oncentration Concentration www.becscreen.com WHY BIOFILM
More information2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose
2017 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility
More information2010 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Children s Hospital
2010 ANTIBIOGRAM University of Alberta Hospital and the Stollery Children s Hospital Medical Microbiology Department of Laboratory Medicine and Pathology Table of Contents Page Introduction..... 2 Antibiogram
More informationDefining Resistance and Susceptibility: What S, I, and R Mean to You
Defining Resistance and Susceptibility: What S, I, and R Mean to You Michael D. Apley, DVM, PhD, DACVCP Department of Clinical Sciences College of Veterinary Medicine Kansas State University Susceptible
More informationVersion 1.01 (01/10/2016)
CHN58: ANTIMICROBIAL SUSCEPTIBILITY TESTING (CLSI) 1.0 PURPOSE / INTRODUCTION: 1.1 Introduction Antimicrobial susceptibility tests are performed in order to determine whether a pathogen is likely to be
More informationMeasure Information Form
Release Notes: Measure Information Form Version 3.0b **NQF-ENDORSED VOLUNTARY CONSENSUS STANDARDS FOR HOSPITAL CARE** Measure Set: Pneumonia (PN) Performance Measure Identifier: Measure Information Form
More informationHelen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory
METHODS USED IN NEW ZEALAND DIAGNOSTIC LABORATORIES TO IDENTIFY AND REPORT EXTENDED-SPECTRUM β-lactamase- PRODUCING ENTEROBACTERIACEAE by Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory
More informationAppropriate antimicrobial therapy in HAP: What does this mean?
Appropriate antimicrobial therapy in HAP: What does this mean? Jaehee Lee, M.D. Kyungpook National University Hospital, Korea KNUH since 1907 Presentation outline Empiric antimicrobial choice: right spectrum,
More informationStreptococcus pneumoniae. Oxacillin 1 µg as screen for beta-lactam resistance
Streptococcus pneumoniae Oxacillin µg as screen for beta-lactam resistance Version 6. June Streptococcus pneumoniae and zone diameter correlates The following histograms present inhibition zone diameter
More informationDetecting / Reporting Resistance in Nonfastidious GNR Part #2. Janet A. Hindler, MCLS MT(ASCP)
Detecting / Reporting Resistance in Nonfastidious GNR Part #2 Janet A. Hindler, MCLS MT(ASCP) Methods Described in CLSI M100-S21 for Testing non-enterobacteriaceae Organism Disk Diffusion MIC P. aeruginosa
More informationSAMPLE. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals
VET01 5th Edition Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals This standard covers the current recommended methods for disk diffusion
More informationPROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains
PROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains 1 INTRODUCTION... 1 2 OBJECTIVES... 2 3 OUTLINE OF THE EQAS 2017... 2 3.1 Shipping, receipt and storage of strains...
More informationOver the past several decades, the frequency of. Resistance Patterns Among Nosocomial Pathogens* Trends Over the Past Few Years. Ronald N.
Resistance Patterns Among Nosocomial Pathogens* Trends Over the Past Few Years Ronald N. Jones, MD Multiple surveillance studies have demonstrated that resistance among prevalent pathogens is increasing
More information2015 Antibiogram. Red Deer Regional Hospital. Central Zone. Alberta Health Services
2015 Antibiogram Red Deer Regional Hospital Central Zone Alberta Health Services Introduction. This antibiogram is a cumulative report of the antimicrobial susceptibility rates of common microbial pathogens
More informationAntibiotic Susceptibility of Common Bacterial Pathogens in Canine Urinary Tract Infections
Vol.1 No.2 Oct-Dec 2013 ISSN : 2321-6387 Antibiotic Susceptibility of Common Bacterial Pathogens in Canine Urinary Tract Infections S. Yogeshpriya*, Usha N.Pillai, S. Ajithkumar and N. Madhavan Unny Department
More informationESCMID Online Lecture Library. by author
Expert rules in susceptibility testing EUCAST-ESGARS-EPASG Educational Workshop Linz, 16 19 September, 2014 Dr. Rafael Cantón Hospital Universitario Ramón y Cajal SERVICIO DE MICROBIOLOGÍA Y PARASITOLOGÍA
More informationUpdate on the CLSI Standards for Antimicrobial Susceptibility Testing: What s New with the Gram Positive Organisms?
2 0 1 3 CASES (with answers!) Update on the CLSI Standards for Antimicrobial Susceptibility Testing: What s New with the Gram Positive Organisms? Susan E. Sharp, Ph.D., DABMM, FAAM Director - Regional
More informationClinical Usefulness of Multi-facility Microbiology Laboratory Database Analysis by WHONET
Special Articles Journal of General and Family Medicine 2015, vol. 16, no. 3, p. 138 142. Clinical Usefulness of Multi-facility Microbiology Laboratory Database Analysis by WHONET Sachiko Satake, PhD,
More informationDrug resistance analysis of bacterial strains isolated from burn patients
Drug resistance analysis of bacterial strains isolated from burn patients L.F. Wang, J.L. Li, W.H. Ma and J.Y. Li Inner Mongolia Institute of Burn Research, The Third Affiliated Hospital of Inner Mongolia
More information2009 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Childrens Hospital
2009 ANTIBIOGRAM University of Alberta Hospital and the Stollery Childrens Hospital Division of Medical Microbiology Department of Laboratory Medicine and Pathology 2 Table of Contents Page Introduction.....
More informationSMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* ...
SMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* The next-generation MicroScan WalkAway System combines proven technology and reliability with enhanced ease-of-use features to streamline
More informationComparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria
Comparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria Juhee Ahn Department of Medical Biomaterials Engineering Kangwon National University October 23, 27 Antibiotic Development
More informationTaiwan Surveillance of Antimicrobial Resistance (TSAR)
Taiwan Surveillance of Antimicrobial Resistance (TSAR) 2009 MIRL Symposium July 17, 2009 Tsai-Ling Yang Lauderdale ( ) Microbial Infections Reference Laboratory (MIRL) Division of Infectious Diseases,
More informationAntimicrobial susceptibility
Antimicrobial susceptibility PATTERNS Microbiology Department Canterbury ealth Laboratories and Clinical Pharmacology Department Canterbury District ealth Board March 2011 Contents Preface... Page 1 ANTIMICROBIAL
More informationQuality Control Testing with the Disk Antibiotic Susceptibility Test of Bauer-Kirby-Sherris-Turck
Quality Control Testing with the Disk Antibiotic Susceptibility Test of Bauer-Kirby-Sherris-Turck DONNA J. BLAZEVIC, M.P.H., MARILYN H. KOEPCKE, B.S., A JOHN M. MATSEN, M.D. Departments of Laboratory Medicine
More information