Supplementary Table S1. Clinical score index used in this study.
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1 Supplementary Table S. linical score index used in this study. General Sensory Facial Lesions Symptom (points) bsent (0) Mild () Moderate (2) Severe () Responsive pathy, sheep react only if (sheep is alert and epression Sheep is listless stimulated, separating from responsive to the flock environmental stimuli) norexia Salivation Normal feeding No secretion Not responding when food is provide Lower jaw wet from saliva Facial oedema bsence of oedema Local oedema Muco-cutaneous and oral lesions No lesions Hyperemia and inflammation labial mucosa/gums, blisters appear on oral mucosa Only minimal feeding during the day Sheep is actively secreting Multiple oedemas localized in different regions Petechial and ecchymotic hemorrhages in the oral mucosa/oral erosions Prostration, not responsive to stimuli No eating at all Hypersalivation Marked facial oedema, bottle jaw (submandibular oedema) onfluent erosions oral mucosa/ulcers Ocular discharge bsence of ocular clinical signs Lacrimation/ red mucous membranes ischarge/conjunctivitis ataractal, white haze, conjunctivitis Respiratory symptoms Fever Veterinary intervention nimal eath Feet lesions ermal and hoof coronet lesions Lower airway distress Nasal discharge Upper airway distress No lesions/normal derma Normal respiratory rate* ( breath/min) bsence of ocular/nasal clinical signs Normal breathing ody temperature below Reddening of skin above coronary band, sheep doesn t show any difficulty of movements Slightly increased respiratory rate* (4-60) and/or abnormal breathing sounds Intermittent serous (clear) to mucoid (gray and cloudy) discharge/lacrimation Rare coughing/sneezing (only during physical activity) ody temperature equal or above oronitis, warm lower limbs and hooves (body mass is moved to other legs) Increased respiratory rate* (6-0) and/or clear abdominal breathing Persistent mucoid (gray and cloudy) to purulent (thick and yellowish/green) discharge Frequent coughing/sneezing (also at rest) ody temperature equal or above 4 Severe lameness, stiff gait, hunched appearance/sheep may kneel/lying down/crippled Strongly increased respiratory rate* (>0) and/or rapid abdominal breathing and/or mouth breathing/abnormal breathing sound (froth) Purulent (thick and yellowish/ green) to bloody discharge Frequent coughing/sneezing at rest with prolonged episodes / 8 Points 8 Points
2 Supplementary Figure S (log/µg RN) G H 2 F I 2 4 E (log/µg RN) (young) L 2 Supplementary Figure S. Experimental infection of goats and different sheep breeds with TV8NET06. ody (rectal) temperature, and levels of TV RN in blood of experimentally infected sheep and goats. Panels show the data of each experimentally infected animal within the indicated groups. Physiological temperature in sheep ranges between 8. and.⁰ and are shown as broken lines.
3 Supplementary Figure S Fever General 4 8 Total ntibody titre (log P0 /ml) (log/µg RN) Respiratory linical score value Supplementary Figure S2. No clinical signs, fever, viremia or neutralizing antibodies in mock-infected control goats and sheep. ata obtained in mock-infected goats and sheep used in this study.. linical scores.. ody (rectal) temperature (average per group). Physiological temperature in sheep ranges between 8. and.⁰ and are shown as broken lines.. TV RN in blood.. Neutralizing antibodies towards TV.
4 Supplementary Figure S TV-2IT00 TV-8NET06 TV-8IT (log/µg RN) E F 2 Supplementary Figure S. Virulence of TV-2IT00, TV-8NET06 and TV-8IT08. ody (rectal) temperature, and levels of TV RN in blood of experimentally infected sheep and goats. Panels show data obtained in each experimentally infected sheep within the indicated groups infected with either TV-2IT00, TV-8NET06 or TV-8IT08. Physiological temperature in sheep ranges between 8. and.⁰ and are shown as broken lines.
5 Supplementary Figure S4 TV-8NET0(blood) TV-8NET0(K-2HK) (log/µg RN) Supplementary Figure S4. Experimental infection of sheep with TV-8NET0(blood) and TV-8NET0(K-2HK). ody (rectal) temperature, and levels of TV RN in blood of experimentally infected sheep and goats. Panels show data obtained in each experimentally infected sheep within the indicated groups infected with either TV-8NET0(blood) or TV-8NET0(K-2HK). Physiological temperature in sheep ranges between 8. and.⁰ and are shown as broken lines.
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