Research Administrators Management Program. Use of Animal Subjects (IACUC) Judy Lassiter, Regulatory Compliance Administrator

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1 RAMP Research Administrators Management Program Use of Animal Subjects (IACUC) Judy Lassiter, Regulatory Compliance Administrator

2 Objectives A Brief History of Animal Research Oi Origin i of fregulations Legal Requirements Ethical Responsibilities IACUC Oversight

3 A Brief History of Animal Research Rome, 150 C.E. - Galen studied animal physiology to better understand human physiology

4 1600 C.E. - William Harvey Through study of both animal and human cadavers, explains the circulation of blood

5 Advances against lethal diseases: Smallpox

6 Anthrax and Rabies

7 Malaria

8 Management of Diabetes

9 1940 s, 1950 s Post WWII, revelations from Nuremberg trials, Nuremberg Code 1950 The Animal Care Panel (now AALAS) Development of Polio vaccine

10 1960 s 1961: Animal Care Technician Certification Board of the Animal Care Panel : Development of the first Guide for Laboratory Animal Facilities and Care 1964:The Animal Facilities Accreditation Board of the Animal Care Panel becomes the American Association for Accreditation ti of Laboratory Animal Care (AAALAC).

11 1965: Pet Theft & Regulations In 1965, a pet Dalmatian (Pepper) owned by a New York family was missing. The owner recognized his dog in a news photograph of a group of dogs being transported for eventual resale to biomedical research institutions. The dog owner was refused entry to the holding facility by the dealer Appeals by his congressman were also refused; the dog was sacrificed as part of an experiment by a NYC hospital Unhappy, the congressman introduced a bill to regulate the trade of dogs. A similar bill was introduced in the Senate simultaneously. Both bills had only marginal support and might have died in committee except for a supporting event

12 February 1966 Life Magazine Concentration Camps for Dogs A raid on a dog dealer's premises by a Humane Society of the United States agent and the Maryland State Police plays a significant role in creating public pressure for the enactment of the federal Laboratory Animal Welfare Act of 1966

13 Legal Obligations

14 Animal Welfare Act A series of laws regulating animal use activities. The enforcing body of the animal welfare act is the United States Department of Agriculture. Violation of the Act is punishable under federal law and could lead to research program suspensions, fines and/or imprisonment.

15 Public Health Service (PHS) Policy on Humane Care and Use of Laboratory Animals Any time The Public Health Service funds research involving animals the PHS Policy is applicable. The Office of Laboratory Animal Welfare, National Institutes of Health administers the PHS Policy through the Research Extension Act. Deviation from the PHS Policy could result in loss of funding from Public Health Service organizations.

16 What do these regulations and policies ensure? That animals are used in research only when it s absolutely necessary. That when animals are used in research they are humanely treated.

17 When can animals be used? 1. When there are no other alternatives. 2. When confirmation has been made that research activities are not unnecessarily duplicating previously conducted experiments. 3. Experiments involving animals are relevant to human or animal health, will advance scientific knowledge, or will be for the good of society.

18 Ethical responsibilities when using animals Animal users have an ethical responsibility to respect and treat animals humanely They also have an ethical responsibility to avoid or minimize the pain and distress animals may endure when used in research or teaching The Regulations and Policies help to ensure animals are treated t humanely.

19 How? The law specifically states: 1. When animals are used for research a scientist must avoid or minimize discomfort, distress, and painful situations. 2. If a procedure involves more than momentary or slight pain or distress, it must be performed using appropriate pain relieving drugs (e.g. sedatives, analgesia or anesthesia). 3. If animals are to be transported, appropriate arrangements must be made to ensure the process is comfortable and occurs with as little stress as possible. 4. The living conditions of animals must be clean and appropriate for 4. The living conditions of animals must be clean and appropriate for the species.

20 Guidelines and Regulations used to ensure animals are humanely treated 1. Guide for Care and Use of Laboratory Animals 2. The Guide for Care and Use of Agriculture Animals in Agricultural l Research and Teaching 3. Report of the AVMA Panel on Euthanasia 4. Guidelines for the Use of Fish in Research 5. USDA Animal Care and Use Policies 6. Animal Welfare Act Regulations 7. Public Health Services Policy

21 Example: Recommended Space for Commonly Used Group-Housed Laboratory Rodents TABLE 2.1 (Guide for Care and Use of Laboratory Animals, page 27) Animals Weight, g Floor Area/Animal, in2 Height, in Mice < Up to Up to >25d >15 5 Rats < Up to Up to Up to Up to >500d >70 7

22 Regulatory Oversight How do federal and state agencies ensure the regulatory requirements are satisfied?

23 Institutional Animal Care and Use Committees (IACUCs) A committee comprised of faculty and staff and empowered by law to ensure all research activities involving animals satisfy federal, state and local regulations & policies governing the use of animals in research. The committee is comprised of at least 5 members: Chairperson Veterinarian Scientist - Must be experienced in using research animals. Non-Scientific Member Conducts protocol evaluations not based on the science. Nonaffiliated Member Represents the general interests of the community.

24 What is the Charge of the IACUC? The IACUC is a University Governance committee, legally required to oversee all animal care and use activities conducted at their institution.

25 How does the committee satisfy its responsibilities? The IACUC reviews and approves all research, teaching or testing activities that involve animals before scientists begin their experiments to ensure: there are no alternatives to using animals, that research is not being unnecessarily duplicated, and th t th i t i l t t h that the experiment is relevant to human or animal health and will be for the good of society.

26 IACUC Oversight Review of animal use procedures: Reviews and approves all procedures to be conducted on animals. Identify potentially painful and stressful procedures and ensure medications are being used to eliminate or minimize the pain/distress. Monitor the research activities ities to ensure only IACUC reviewed ed & approved procedures res are being conducted on animals. Inspect the living quarters of animals at least twice a year to ensure: Animals are checked daily that, their housing facilities are clean and that they regularly receive fresh food & water. They are appropriate for the species being housed. Animals are receiving appropriate veterinary care. The IACUC also ensures: The number of animals used for the research will yield statistically valid results. The appropriate specie of animal is being used for the project. Humane experimental endpoints have been established. Appropriate methods of euthanasia are being utilized.

27 Peers Providing Oversight at their Own Institution? Does this sound like the fox guarding the hen house?

28 External Oversight of IACUCs Remember one IACUC member is a nonaffiliated member and is acting on behalf of the community. Reports made to federal agencies are available to the public for review. Opponents of animal research will do everything in their power to hold research institutions accountable to federal regulations and policies i

29 The Bottom Line 1. Using animals for research activities is a privilege, not a right. 2. It is a privilege that a scientist or an institution tion can lose if the ethical (e.g. legal and moral) responsibilities are not satisfied.

30 We are here to help you It is best to defer to the IACUC office for a determination on whether or not review is required. We re here to assist you and the investigators. If we don t know the answers, we probably know who does! Don t hesitate to contact us with your questions.

31 Online Informational Resources Available through NCSU NCSU Animal Subjects Tutorial (

32 Questions? Judy Lassiter, Certified Professional IACUC Administrator, , Paula DeLong, AALAS Certified Laboratory Animal Technologist, , Dr. Steve Dempsey, Assistant Vice Chancellor & University Attending Veterinarian, ,

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