Verticillium wilt in a cotton variety test at the Judd Hill Cooperative Research Station in 2017

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1 Arknss 2017 Cotton Vriety Test Verticillium wilt in cotton vriety test t the Judd Hill Coopertive Reserch Sttion in 2017 F. Bourlnd W. Brnett C. Kennedy L. Mrtin A. Rouse nd B. Robertson ARKANSAS AGRICULTURAL EXPERIMENT STATION Mrch 2018 Reserch Series 650

2 This publiction is vilble on the internet t: nd t Technicl editing nd cover design by Gil Hlleck. Photo Credits: Photo tken on September 7 (prior to defolition) shows hevy incidence of Verticillium wilt in cotton vriety test t the Judd Hill Coopertive Reserch Sttion, ner Trumnn, Ark. in Verticillium wilt is cused by soilborne fungus, which infects the plnt through its roots nd cn progressively plug the vsculr system of the plnt. The disese cn result in chlorotic nd bscissed leves, premture boll opening, reduced yield, nd deth of the plnt. Fred Bourlnd, Arknss Agriculturl Experiment Sttion, University of Arknss System, Division of Agriculture. Arknss Agriculturl Experiment Sttion, University of Arknss System Division of Agriculture, Fyetteville. Mrk J. Cochrn, Vice President for Agriculture; Jen-Frnçois Meullenet, Interim AAES Director nd Associte Vice-President for Agriculture Reserch. WWW/CC2018. The University of Arknss System Division of Agriculture offers ll its Extension nd Reserch progrms nd services without regrd to rce, color, sex, gender identity, sexul orienttion, ntionl origin, religion, ge, disbility, mritl or vetern sttus, genetic informtion, or ny other leglly protected sttus, nd is n Affirmtive Action/Equl Opportunity Employer. ISSN: CODEN: AKAMA6

3 Arknss Cotton Vriety Test 2017 F. Bourlnd W. Brnett C. Kennedy L. Mrtin A. Rouse B. Robertson Arknss Agriculturl Experiment Sttion University of Arknss System Division of Agriculture Fyetteville, Arknss 72704

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5 Summry The primry gol of the Arknss Cotton Vriety Test is to provide unbised dt regrding the gronomic performnce of cotton vrieties nd dvnced breeding lines in the mjor cotton-growing res of Arknss. This informtion helps seed compnies estblish mrketing strtegies nd ssists producers in choosing vrieties to plnt. These nnul evlutions will then fcilitte the inclusion of new, improved genetic mteril in Arknss cotton production. Adpttion of vrieties is determined by evluting the lines t five University of Arknss System Division of Agriculture reserch sites (Mnil, Keiser, Judd Hill, Mrinn, nd Rohwer). Entries in the 2017 Arknss Cotton Vriety Test were evluted in two groups trnsgenic nd conventionl vrieties. The 41 entries in the trnsgenic test included 18 entries (13 B2XF, 2 WRF, 2 GLT, nd 1 GLB2) returning from the 2016 test nd 23 first-yer entries (6 B2XF, 3 B3XF, 1 GLT, nd 13 W3FE) nd were evluted t ll five loctions. The conventionl test included 16 entries nd ws evluted t ll loctions except Mnil. Reported dt include lint yield, lint percentge, plnt height, percent open bolls, yield component vribles, fiber properties, lef pubescence, stem pubescence, nd brct trichome density. All entries in the experiments were evluted for response to trnished plnt bug nd bcteril blight in seprte tests t Keiser. This 2017 report includes results of lrge-plot vriety tests in 7 counties tht were coordinted by Bill Robertson. Contents Introduction...4 Mterils nd Methods...4 References...7 Acknowledgements...7 Prticipnts nd entries in the 2017 Arknss Cotton Vriety Test (Tble 1)...8 Production informtion for ll loctions (Tble 2)...9 Environmentl conditions (Tble 3)...9 Results Trnsgenic Vriety Test: All loctions (Tbles 4-5)...10 Mnil (Tbles 6-7)...12 Keiser (Tbles 8-9)...14 Judd Hill (Tbles 10-11)...16 Mrinn (Tbles 12-13)...18 Rohwer (Tbles 14-15)...20 Morphologicl nd host-plnt resistnce trits (Tble 16) yer nd 3-yer yield verges (Tble 17)...23 Conventionl Vriety Test: All loctions (Tbles 18-19)...24 Keiser (Tbles 20-21)...25 Judd Hill (Tbles 22-23)...26 Mrinn (Tbles 24-25)...27 Rohwer (Tbles 26-27)...28 Morphologicl nd host-plnt resistnce trits (Tble 28) yer nd 3-yer yield verges (Tble 29)...30 County Lrge-Plot, Replicted Vriety Evlution: Appendix Tbles A1-A

6 Arknss Cotton Vriety Test 2017 F. Bourlnd, W. Brnett, C. Kennedy, L. Mrtin, A. Rouse, nd B. Robertson 1 Introduction T he purpose of the University of Arknss Cotton Vriety Testing Progrm is to provide unbised comprisons of cotton vrieties nd dvnced breeding lines over rnge of environments. Dt from these tests help to identify the potentil dptbility of vrieties to prticulr cotton-growing regions of the stte. Bourlnd et l. (2000) documented severl unintentionl bises, which re inherent to the Arknss cotton vriety testing progrm. These include mngement ssocited with vrieties expressing herbicide nd insect resistnce. The bises tend to cncel ech other so tht no gret dvntge is given to ny prticulr vriety. Since evlution of genetic differences mong entries is the ultimte gol of the evlutions, ll vrieties re treted identiclly within the primry loctions (Mnil, Keiser, Judd Hill, Mrinn, nd Rohwer) of the vriety test. No specilized production inputs were employed with respect to the vrious geneticlly enhnced vrieties. All entries in the tests t Mnil possessed the RF or G genes, nd were uniformly treted with Round-up. Since the plots were over-spryed with Roundup, the conventionl vrieties were not evluted t Mnil. Mterils nd Methods The 41 entries in the trnsgenic test included 18 entries (13 B2XF, 2 WRF, 2 GLT, nd 1 GLB2) returning from the 2016 test nd 23 first-yer entries (6 B2XF, 3 B3XF, 1 GLT, nd 13 W3FE) (Tble 1). The trnsgenic test ws replicted 6 times t Mnil, 5 times t Judd Hill, nd 4 times t Keiser, Mrinn, nd Rohwer. The conventionl test included 16 entries nd ws evluted using 5 replictions t ech loction. Test sites included the Northest Reserch nd Extension Center t Keiser; the Judd Hill Coopertive Reserch Sttion t Judd Hill (ner Trumnn); the Lon Mnn Cotton Reserch Sttion t Mrinn; the Mnil Airport Cotton Reserch Frm t Mnil; nd the Rohwer Reserch Sttion t Rohwer. Culturl prctices nd wether dt (het units nd rinfll) ssocited with the test sites re listed in Tble 2 nd Tble 3, respectively. Origintors of seed supplied double-treted (two fungicides) seed for ll entries. Prior to plnting, ll seed were treted with imidcloprid (Gucho ) t rte of 6 oz/100 lb seed. Plots were plnted with constnt number of seed (bout 4 seed/row ft). All vrieties were plnted in tworow plots on 38-inch centers nd rnged from 40 to 50 feet in length. Experiments were rrnged in rndomized complete block. Although exct inputs vried cross loctions, culturl inputs t ech loction were generlly bsed on University of Arknss System Division of Agriculture Coopertive Extension Service recommendtions for cotton production, including COTMAN rules for insecticide termintion. All plots were mchine-hrvested with 2-row or 4-row cotton pickers modified with lod cells for hrvesting smll plots. Dt Collected t Single Loction Lef Pubescence. Lef pubescence ws visully rted on scle of 1 (smooth lef) to 9 (pilose, very hiry) in the irrigted experiments t Keiser using the system described by Bourlnd et l. (2003). A full-sized minstem lef locted bout 5-6 nodes from plnt pex ws rted for 6 plnts per plot for ll 5 replictions during August. Stem Pubescence. Stem pubescence ws visully rted on scle of 1 (smooth stem) to 9 (very hiry) in the irrigted experiments t Keiser using system similr to tht used for leves. After hrvest, the upper 5-6 inches of the plnt pex ws rted for 6 plnts per plot for ll 5 replictions. Brct Trichomes. As ll plnts pproched physiologicl cutout, brct from 1st position white flower ws smpled from 6 rndom plnts per plot (4 of the 5 replictions) in the Keiser experiments. Ech brct ws exmined for mrginl trichome density (no. of trichomes/ cm) s described by Bourlnd nd Hornbeck (2007). Mens for the 6 brcts were evluted s plot mens. 1 F. Bourlnd is professor, nd W. Brnett nd A. Rouse re progrm technicins t the Northest Reserch nd Extension Center; C. Kennedy is resident director t the Lon Mnn Cotton Reserch Sttion; L. Mrtin is progrm technicin t the Rohwer Reserch Sttion; nd B. Robertson is n Extension Cotton Agronomist t the Newport Extension Center. 4

7 Arknss Cotton Vriety Test 2017 Trnished Plnt Bug. Entries in both vriety tests were evluted for response to TPB in seprte field t Keiser. The TPB test included 8 replictions of 1-row plots (20-feet long on 38-inch wide rows). Four rows of highly susceptible Frego-brct line were plnted between the tests on April 14. The TPB tests were plnted on My 15 nd received no insecticide tretment for TPB infesttions. Erly flowering in the susceptible Frego-brct strips encourged TPB popultions to increse, then to migrte from the strips s the test plots begn to flower. Response to TPB ws determined by exmining white flowers (6 flowers/plot/dy for 6 dys in lte August) for presence of nther dmge. Accumultive percentge of dmged flowers ( dirty flowers ) ws determined for ech plot. Bcteril Blight. Entries in both tests were plnted in flts (2 replictions, 13 seed/plot) in the greenhouse, nd scrtch inoculted with Xnthomons citri pv. mlvcerum. The inoculum ws obtined from nturlly infected leves collected t the 2015 Mnil loction. Scrtches were exmined for wter-soking, nd percent of susceptible plnts ws determined. Verticillium Wilt. Reltive yields of vrieties over yers t Judd Hill should be indictive of tolernce to Verticillium wilt. Dt Collected t All Loctions Plnt Height. Plnt height mesurements (in cm) were collected fter plnts hd cutout. Averge plnt heights for vrieties were determined by mesuring from the soil surfce to the terminl of one verge-sized plnt in ech of the two rows. Plot mens (verge of the two mesurements) were evluted. Plnt height ws not mesured t Mrinn in % Open Bolls. After first ppliction of defolints, percentge of open bolls ws estimted from the front nd bck of ech plot, then verged for ech plot. Boll Smples nd Lint Percentge. Prior to mechnicl hrvest, hnd-hrvested smples of 40 open bolls were obtined from 2 replictions t ech loction. Within ech row of 2-row plots, site hving verge or bove verge plnt density ws chosen nd 20 bolls (5 bottom, 10 mid-cnopy nd 5 top bolls) were hrvested nd bulked to form 40-boll smple. The 40-boll smples were ginned (lb gin without the use of lint cleners) to determine lint frction (the percentge of lint weight to seed cotton weight). Fiber Properties. Fiber smples were tken from ech boll smple nd were evluted using HVI clssifiction. Prmeters included micronire, fiber length, length uniformity index (UI), strength, nd elongtion. To reflect mrket demnd for fiber qulity, weighted qulity score (Q-score) ws clculted s described by Bourlnd et l. (2010). Prmeters (nd weighting) included in Q-score were fiber length (50%), micronire (25%), length uniformity index (15%), nd strength (10%). Seed Index. Two sets of 25 fuzzy seed from the ginned seed of ech 40-boll smple were counted nd weighed. If the two weights vried more thn 0.2 g, second set of smples ws tken. Two consistent weights of 25 seed were used to clculte fuzzy seed index (weight of 100 seed). Seed Per Acre. For ech plot, n estimte of number of seed per cre ws determined by multiplying seed cotton yield (lb/a converted to g/a) times verge seed percentge (the percentge of seed weight to seed cotton weight in ginned smple, verged by entry nd loction over reps), then divided by verge seed weight (verge seed index by entry over reps divided by 100). Lint Index. Lint index (weight of lint on 100 seed) ws determined from 40-boll smple dt by dividing lint weight from ginned smple by the number of seed per smple (estimted using verge seed weight) then multiplying by 100. Fibers Per Seed. Fibers per seed were estimted by dividing lint index by n estimted weight of individul fibers. Weight of n individul fiber ws estimted by: (fiber length length uniformity (micronire/1,000,000)). Fiber Density. Fiber density, reported s the number of fibers per mm 2, ws estimted by dividing fibers per seed by seed surfce re. Seed surfce re (SSA) ws estimted by the regression eqution suggested by Groves nd Bourlnd (2010): SSA = SI, where SI is equl to seed index ssocited with the smple. Lint Yield. Seed cotton yield per plot (determined by mechnicl cotton picker) ws converted to seed cotton yield per cre then multiplied by verge lint percentge (determined by vriety nd loction) to estimte lint per cre. Yield Comprisons Uncontrolled vrition is inherent to collection of vriety performnce dt (prticulrly yield dt). In ddition to their genetic bility, vrition mong vrieties my be due to slight differences in soil, pest or climtic conditions within field, vrious interctions with specific mngement prctices, or experimentl error. Sttistics llow users to define the degree of uncontrolled vrition nd to in- 5

8 AAES Reserch Series 650 terpret dt. The sttisticl tool used to compre mens in these tests ws Fisher s Protected Lest Significnt Difference (LSD). An LSD ws clculted when the F-test vlue from nlysis of vrince ws significnt. Yields of vrieties re considered significntly different if the difference between men yields of two vrieties is greter thn the LSD vlue. Differences tht re smller thn the LSD my hve occurred by chnce or my be ssocited with uncontrolled vrition, nd re therefore considered not significnt. Additionl estimtes of vrition re provided by mesures of R-squred nd coefficient of vrition (CV). R- squred (times 100) indictes the percentge of vrition tht is explined by defined sources of vrition (e.g., repliction nd vriety effects within loction). Confidence in dt increses s R-squred increses. Generlly, the meningfulness of difference mong mens is questionble when dt hve R-squred vlues of less thn 50%. Also, confidence in dt becomes greter s CV declines. Results Entries nd prticipnts in the test re listed in Tble 1. Culturl inputs nd production informtion for vriety trils t Mnil, Keiser, Judd Hill, Mrinn, nd Rohwer re reported in Tble 2. Tble 3 includes wether informtion for north, centrl, nd south Arknss loctions during the 2017 production seson. The Rohwer loction ws plnted on April 25, but rin delyed plnting t ll other loctions (Tble 2). Monthly het unit ccumultion ws ner norml t Keiser nd Mrinn from My through July, then cooler thn norml in August nd September, nd wrmer thn norml in October (Tble 3). Tempertures t Rohwer were cooler thn norml from My through August, then wrmer thn norml in September nd October. Except for October, rinfll ws higher thn norml through the seson t ech loction. Rinfll in August ws prticulrly high-more thn twice the norml mount t ech loction. Hrvest conditions were optimum t ech loction. Performnce dt of entries in the 2017 Arknss Trnsgenic Cotton Vriety Test t Mnil, Keiser, Judd Hill, Mrinn nd Rohwer re provided in Tbles 4 through 15 with yield nd yield-relted vribles in the even-numbered tbles nd fiber properties in the odd-numbered tbles. Performnce dt cross ll 5 loctions re presented in Tbles 4 nd 5. Morphologicl nd host-plnt resistnce mesurements for the trnsgenic entries re in Tble 16. Two- nd three-yer yield mens for entries evluted in previous yers re in Tble 17. Performnce dt of entries in the 2017 Arknss Conventionl Cotton Vriety Test t Keiser, Judd Hill, Mrinn nd Rohwer re provided in Tbles 18 through 27 with yield nd yield-relted vribles in the evennumbered tbles nd fiber properties in the odd-numbered tbles. Morphologicl nd host-plnt resistnce mesurements for the conventionl entries re in Tble 28. Two- nd three-yer yield mens for the conventionl entries evluted in previous yers re in Tble 29. Other observtions ssocited with ech test site include: Mnil (Tbles 6 nd 7). The test t Mnil ws plnted in the sme field used since 2014, nd in the sme re of the field used since In nticiption of high field vribility, the test ws replicted 6 times with 3 tiers used per repliction. The R-squred vlues for lint yield were reltively low, but reltive yield differences mong the vrieties t Mnil were similr to other loctions. Mepiqut chloride (totl of 64 oz/a) ws used to control plnt height. Keiser (Tbles 8, 9, 20 nd 21). To help combt resistnt pigweed, cerel rye cover crop ws plnted to the field in the fll of The cover crop grew well nd ws terminted in mid-april. Unfortuntely, excessive rins nd wind cuse much of the cover crop to bend over, nd delyed drying of soil. We subsequently decided to mow the cover crop nd plnt the tests on My 11 in the residue. Resulting stnds were not s uniform s desired, nd plnts did not grow t n cceptble rte. Pigweeds were hnd-hoed throughout the seson. Mlfunctioning of lod cells on the plot picker postponed hrvest, nd subsequent rins delyed hrvest until October 31. Mepiqut chloride ws not pplied to the plots.. Judd Hill (Tbles 10, 11, 22 nd 23). The Judd Hill plots were plnted on My 11 nd chieved excellent stnds. The plnts grew well nd estblished excellent boll lods. With reltive cool tempertures nd mple moisture in August, the boll-loded plnts becme hevily infected with Verticillium wilt. Resulting wilt symptoms were s severe s we hve ever seen t Judd Hill. Just s plots were redy for hrvest in lte September, the engine of the plot picker filed, nd hd to be subsequently replced. Hrvest ws delyed until November 14. Mepiqut chloride (26 oz/a) ws pplied erly in seson to control plnt height. Mrinn (Tbles 12, 13, 24 nd 25). For the second yer, we used cerel rye cover crop in our tests t Mri- 6

9 Arknss Cotton Vriety Test 2017 nn. The cover crop worked very well in 2016, but nitrogen pplied to the cover crop cused excessive growth in Unfortuntely, the excessive cover ws not killed in timely mnner, nd we hd difficulty plnting the plots. Without GPS formed rows, it ws impossible to sty on top of beds while plnting. The excessive cover mde it difficult to get proper plcement of seed, nd subsequently tied up nitrogen throughout the seson. Results stnds were reltively poor, nd plnts did not develop properly. Mepiqut chloride (totl of 2.5 oz/a) ws used to control plnt height. Rohwer (Tbles 14, 15, 26 nd 27). The Rohwer loction ws plnted on April 25, nd excellent stnds were chieved. The plnts grew well, nd produced good yields. The field ws scheduled to be hrvested in September, but the 4-row plot picker burned just before hrvesting our field. The picker ws completely destroyed nd hd to be replced. Hrvest ws delyed until October 17. Mepiqut chloride (totl of 40 oz/a) ws used to control plnt height. References Bourlnd, F.M., N.R. Benson, nd W.C. Robertson Inherent bises in the Arknss cotton vriety testing progrm. pp In Proc. Beltwide Cotton Prod. Res. Conf., Sn Antonio, Texs. 4-8 Jn Ntionl Cotton Council, Memphis, Tenn. Bourlnd, F.M., R. Hogn, D.C. Jones, nd E. Brnes Development nd utility of Q-score for chrcterizing cotton fiber qulity. J. Cotton Sci. 14: Avilble t JCS14-53.pdf Bourlnd, F.M., J.M. Hornbeck, A.B. McFll, nd S.D. Clhoun A rting system for lef pubescence of cotton [Online]. J. Cotton Sci. 7:8-15. Avilble t Bourlnd, F.M. nd J.M. Hornbeck Vrition in mrginl brct trichomes on Uplnd cotton. J. Cotton Sci. 11: Avilble t jounl/2007/11/4/242.cfm Groves, F.E., nd F.M. Bourlnd Estimting seed surfce re of cottonseed. J. Cotton Sci. 14: Avilble t 2/uplod/JCS14-74.pdf Acknowledgments We express our pprecition to the Directors, Progrm Technicins nd stff t the University of Arknss System Division of Agriculture s Northest Reserch nd Extension Center, Lon Mnn Cotton Reserch Sttion, nd the Rohwer Reserch Sttion. Annully, the Judd Hill Foundtion generously provides the test site for experiments t Judd Hill. We re prticulrly grteful to the City of Mnil for mking lnd vilble for testing, nd to the Mississippi County Coopertive Extension Agents nd Wildy Frms for ssisting with the test site t the Mnil Airport. Annul evlution of cotton vrieties is mde possible by the work of the reserch ssistnts nd technicins t these loctions, nd by the contributions of seed compnies prticipting in the Arknss Cotton Vriety Test. 7

10 AAES Reserch Series 650 Tble 1. Prticipnts nd entries in the 2017 Arknss Cotton Vriety Test. Institution/Contct personreturning entries Experimentl no. 1st yer entries Experimentl no. Americot Inc. AMX 1711 B2XF NG3406 B2XF AMX 1715 B2XF NG3522 B2XF AMDG-7824 NG 3517 B2XF NG 4545 B2XF NG 4601 B2XF Byer Crop Science/ ST 4848GLT BX 1633GLT ST 5020GLT Steve Lee ST 4946GLB2 ST 4949GLT BX 1630GLT Crop Production Services/ Dyn-Gro DG 3385 B2XF DG 3109 B2XF Stcie Bruff DG 3214 B2XF CPS B2XF Monsnto / DP 1518 B2XF 14R925B2XF DP 1823 NR B2XF MON 16R225NRB2XF Dvid Albers DP 1522 B2XF 14R922B2XF DP 1820 B3XF MON 16R324B3XF DP 1612 B2XF MON 14R913B2XF DP 1835 B3XF MON 16R338B3XF DP 1614 B2XF MON 15R515B2XF DP 1639 B2XF MON 14R934B2XF DP 1646 B2XF MON 15R551B2XF DP 1725 B2XF MON 15R535B2XF PhytoGen Seed Co./ PHY 312 WRF PX3122b-51WRF PHY 300 W3FE Chris Min PHY 444 WRF PX WRF PHY 330 W3FE PHY 340 W3FE PHY 450 W3FE PHY 490 W3FE PX2A28W3FE PX3A82W3FE PX3A96W3FE PX3A99W3FE PX4A52W3FE PX4A54W3FE PX4A57W3FE PX4A62W3FE WinField Solutions, LLC / CROPLAN 9608 B3XF WinField United 17XC8 Robert Cossr Conventionl entries Americot Inc. AM UA48 Ark Dyn-Gro Seed/ AT 558 Chrlie Cook Seed Source Genetics/ SSG UA222 Ark Edwrd Jungmnn SSG UA103 Ark SSG UA107 Ark SSG UA114 Ark SSG HQ 210CT Interntionl Seed BRS Technology / BRS Rfel Crvjl BRS University of Arknss / Ark ne Fred Bourlnd Ark Ark Ark Ark Ark

11 Arknss Cotton Vriety Test 2017 Tble 2. Culturl prctices for loctions of the 2017 Arknss Cotton Vriety Test. Loction Input Mnil Keiser Judd Hill Mrinn Rohwer Soil type Routon-Dundee- Shrkey cly Dundee silt lom Cllwy silt lom Hebert silt lom Crevsse complex N, P, K (lbs) Plnting dte 5/19 5/11 5/11 5/9 4/25 Irrigtion method furrow furrow furrow furrow furrow Irrigtion dtes 6/19, 7/18, 8/10 7/18 7/18, 8/07 6/15,6/30,7/12 7/7, 7/15, 7/25, 8/1 7/21, 8/21 Defolition dte 10/2, 10/13 09/17, 10/03 09/28,10/16 9/19,10/3 9/18, 9/28 Hrvest dte 10/24 10/31 11/14 10/13 10/17 Tble 3. Wether summry for the 2017 production seson in north, centrl nd south Arknss. Loction Month DD60s in 2017 Historicl vg. DD60s Rinfll (in.) in 2017 Historicl vg. rinfll Keiser My (northest) June July August September October Totl Mrinn My (centrl) June July August September October Totl Rohwer My (southest) June July August September October Totl DD60 (growing degree dys bsed on 60 ºF) nd rinfll from historicl wether dt from 1960 through

12 AAES Reserch Series 650 Tble 4. Yield nd relted properties 2017 Arknss Cotton Vriety Test cross five test sites. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. DP 1646 B2XF PX4A57W3FE PHY 444 WRF PX4A54W3FE PX3A99W3FE PX4A52W3FE PHY 300 W3FE DP 1823 NR B2XF PX4A62W3FE ST 4949GLT DP 1725 B2XF DG 3385 B2XF DP 1522 B2XF PX3A96W3FE DP 1639 B2XF PHY 312 WRF NG3522 B2XF DP 1820 B3XF PHY 340 W3FE PX3A82W3FE PHY 330 W3FE ST 4946GLB DP 1835 B3XF CROPL B3XF NG 4601 B2XF NG3406 B2XF NG 4545 B2XF CPS B2XF DP 1518 B2XF ST 4848GLT DP 1614 B2XF DG 3214 B2XF PHY 490 W3FE ST 5020GLT DG 3109 B2XF NG 3517 B2XF DP 1612 B2XF PHY 450 W3FE AMX 1711 B2XF AMX 1715 B2XF PX2A28W3FE Men Vr. LSD Loc. LSD ns C.V.% R 2 x Prob (vr x loc) < < <

13 Arknss Cotton Vriety Test 2017 Tble 5. Fiber properties 2017 Arknss Trnsgenic Cotton Vriety Test cross five test sites. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % DP 1646 B2XF PX4A57W3FE PHY 444 WRF PX4A54W3FE PX3A99W3FE PX4A52W3FE PHY 300 W3FE DP 1823 NR B2XF PX4A62W3FE ST 4949GLT DP 1725 B2XF DG 3385 B2XF DP 1522 B2XF PX3A96W3FE DP 1639 B2XF PHY 312 WRF NG3522 B2XF DP 1820 B3XF PHY 340 W3FE PX3A82W3FE PHY 330 W3FE ST 4946GLB DP 1835 B3XF CROPLAN 9608 B3XF NG 4601 B2XF NG3406 B2XF NG 4545 B2XF CPS B2XF DP 1518 B2XF ST 4848GLT DP 1614 B2XF DG 3214 B2XF PHY 490 W3FE ST 5020GLT DG 3109 B2XF NG 3517 B2XF DP 1612 B2XF PHY 450 W3FE AMX 1711 B2XF AMX 1715 B2XF PX2A28W3FE Men Vr. LSD Loc. LSD ns C.V.% R 2 x Prob (vr x loc) < UI = Fiber length uniformity Index. 11

14 AAES Reserch Series 650 Tble 6. Yield nd relted properties 2017 Ark. Cotton Vriety Test, with irrigtion on Routon-Dundee-Crevsse. complex soil t Mnil. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. PX4A57W3FE DP 1646 B2XF ST 4949GLT DP 1823 NR B2XF PX3A99W3FE DP 1725 B2XF DP 1639 B2XF PX4A62W3FE PHY 444 WRF DG 3385 B2XF PX4A54W3FE CPS B2XF CROPL B3XF NG3522 B2XF DP 1820 B3XF PX4A52W3FE PX3A96W3FE DP 1522 B2XF PX3A82W3FE PHY 300 W3FE NG 3517 B2XF DP 1614 B2XF DG 3109 B2XF PHY 340 W3FE PHY 312 WRF ST 4946GLB ST 4848GLT NG 4601 B2XF PHY 330 W3FE DP 1518 B2XF NG 4545 B2XF DG 3214 B2XF DP 1612 B2XF PHY 450 W3FE DP 1835 B3XF AMX 1715 B2XF NG3406 B2XF ST 5020GLT PHY 490 W3FE AMX 1711 B2XF PX2A28W3FE Men LSD C.V.% R 2 x

15 Arknss Cotton Vriety Test 2017 Tble 7. Fiber properties 2017 Arknss Trnsgenic Cotton Vriety Test, with irrigtion on Routon-Dundee-Crevsse complex soil t Mnil. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % PX4A57W3FE DP 1646 B2XF ST 4949GLT DP 1823 NR B2XF PX3A99W3FE DP 1725 B2XF DP 1639 B2XF PX4A62W3FE PHY 444 WRF DG 3385 B2XF PX4A54W3FE CPS B2XF CROPLAN 9608 B3XF NG3522 B2XF DP 1820 B3XF PX4A52W3FE PX3A96W3FE DP 1522 B2XF PX3A82W3FE PHY 300 W3FE NG 3517 B2XF DP 1614 B2XF DG 3109 B2XF PHY 340 W3FE PHY 312 WRF ST 4946GLB ST 4848GLT NG 4601 B2XF PHY 330 W3FE DP 1518 B2XF NG 4545 B2XF DG 3214 B2XF DP 1612 B2XF PHY 450 W3FE DP 1835 B3XF AMX 1715 B2XF NG3406 B2XF ST 5020GLT PHY 490 W3FE AMX 1711 B2XF PX2A28W3FE Men LSD ns C.V.% R 2 x UI = Fiber length uniformity Index. 13

16 AAES Reserch Series 650 Tble 8. Yield nd relted properties 2017 Ark. Cotton Vriety Test, with irrigtion on Shrkey cly soil t Keiser. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. DP 1646 B2XF PHY 300 W3FE PHY 330 W3FE PX4A54W3FE ST 4946GLB PX4A52W3FE PX4A62W3FE DG 3385 B2XF PX3A82W3FE PX3A96W3FE NG3522 B2XF ST 5020GLT ST 4949GLT PX3A99W3FE PHY 340 W3FE DP 1518 B2XF NG 4545 B2XF PHY 312 WRF PX4A57W3FE CPS B2XF NG3406 B2XF DP 1835 B3XF CROPL B3XF PHY 444 WRF PHY 490 W3FE ST 4848GLT PHY 450 W3FE DP 1823 NR B2XF DP 1612 B2XF NG 3517 B2XF DP 1820 B3XF NG 4601 B2XF DG 3109 B2XF DP 1522 B2XF AMX 1711 B2XF DG 3214 B2XF DP 1614 B2XF DP 1725 B2XF AMX 1715 B2XF DP 1639 B2XF PX2A28W3FE Men LSD ns C.V.% R 2 x

17 Arknss Cotton Vriety Test 2017 Tble 9. Fiber properties 2017 Arknss Trnsgenic Cotton Vriety Test, with irrigtion on Shrkey cly soil t Keiser. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % DP 1646 B2XF PHY 300 W3FE PHY 330 W3FE PX4A54W3FE ST 4946GLB PX4A52W3FE PX4A62W3FE DG 3385 B2XF PX3A82W3FE PX3A96W3FE NG3522 B2XF ST 5020GLT ST 4949GLT PX3A99W3FE PHY 340 W3FE DP 1518 B2XF NG 4545 B2XF PHY 312 WRF PX4A57W3FE CPS B2XF NG3406 B2XF DP 1835 B3XF CROPLAN 9608 B3XF PHY 444 WRF PHY 490 W3FE ST 4848GLT PHY 450 W3FE DP 1823 NR B2XF DP 1612 B2XF NG 3517 B2XF DP 1820 B3XF NG 4601 B2XF DG 3109 B2XF DP 1522 B2XF AMX 1711 B2XF DG 3214 B2XF DP 1614 B2XF DP 1725 B2XF AMX 1715 B2XF DP 1639 B2XF PX2A28W3FE Men LSD C.V.% R 2 x UI = Fiber length uniformity Index. 15

18 AAES Reserch Series 650 Tble 10. Yield nd relted properties 2017 Ark. Cotton Vriety Test, with irrigtion on Dundee silt lom soil t Judd Hill. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. PX3A99W3FE DP 1823 NR B2XF PHY 300 W3FE PHY 444 WRF DP 1835 B3XF ST 4848GLT DP 1820 B3XF DP 1522 B2XF PX3A96W3FE DP 1639 B2XF NG3406 B2XF DP 1646 B2XF PX4A54W3FE PHY 312 WRF PX4A57W3FE ST 4949GLT PHY 330 W3FE NG 4601 B2XF NG 4545 B2XF PX2A28W3FE PX4A62W3FE AMX 1711 B2XF DP 1614 B2XF ST 4946GLB NG3522 B2XF PHY 340 W3FE PHY 490 W3FE PX4A52W3FE CROPL B3XF DG 3109 B2XF DP 1725 B2XF PX3A82W3FE DG 3385 B2XF CPS B2XF DG 3214 B2XF ST 5020GLT DP 1518 B2XF PHY 450 W3FE NG 3517 B2XF AMX 1715 B2XF DP 1612 B2XF Men LSD ns C.V.% R 2 x

19 Arknss Cotton Vriety Test 2017 Tble 11. Fiber properties 2017 Arknss Trnsgenic Cotton Vriety Test, with irrigtion on Dundee silt lom soil t Judd Hill. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r Tble 11. Fiber prope lb/a in. % g/tex % PX3A99W3FE DP 1823 NR B2XF PHY 300 W3FE PHY 444 WRF DP 1835 B3XF ST 4848GLT DP 1820 B3XF DP 1522 B2XF PX3A96W3FE DP 1639 B2XF NG3406 B2XF DP 1646 B2XF PX4A54W3FE PHY 312 WRF PX4A57W3FE ST 4949GLT PHY 330 W3FE NG 4601 B2XF NG 4545 B2XF PX2A28W3FE PX4A62W3FE AMX 1711 B2XF DP 1614 B2XF ST 4946GLB NG3522 B2XF PHY 340 W3FE PHY 490 W3FE PX4A52W3FE CROPLAN 9608 B3XF DG 3109 B2XF DP 1725 B2XF PX3A82W3FE DG 3385 B2XF CPS B2XF DG 3214 B2XF ST 5020GLT DP 1518 B2XF PHY 450 W3FE NG 3517 B2XF AMX 1715 B2XF DP 1612 B2XF Men LSD ns C.V.% R 2 x UI = Fiber length uniformity Index. 17

20 AAES Reserch Series 650 Tble 12. Yield nd relted properties 2017 Ark. Cotton Vriety Test, with irrigtion on Cllowy silt lom soil t Mrinn. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. DP 1725 B2XF DG 3214 B2XF DP 1820 B3XF DG 3385 B2XF DP 1522 B2XF PX4A54W3FE NG 4601 B2XF NG 3517 B2XF DP 1612 B2XF DP 1646 B2XF DP 1614 B2XF PX4A57W3FE ST 4848GLT ST 4949GLT CPS B2XF NG3406 B2XF ST 5020GLT PHY 444 WRF NG 4545 B2XF DP 1518 B2XF PX4A62W3FE PX3A96W3FE PHY 312 WRF ST 4946GLB PX3A99W3FE DG 3109 B2XF NG3522 B2XF DP 1835 B3XF DP 1823 NR B2XF PHY 300 W3FE CROPL B3XF DP 1639 B2XF AMX 1715 B2XF PHY 340 W3FE PX3A82W3FE AMX 1711 B2XF PHY 330 W3FE PX4A52W3FE PHY 490 W3FE PHY 450 W3FE PX2A28W3FE Men LSD C.V.% R 2 x

21 Arknss Cotton Vriety Test 2017 Tble 13. Fiber properties 2017 Arknss Trnsgenic Cotton Vriety Test, with irrigtion on Cllowy silt lom soil t Mrinn. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % DP 1725 B2XF DG 3214 B2XF DP 1820 B3XF DG 3385 B2XF DP 1522 B2XF PX4A54W3FE NG 4601 B2XF NG 3517 B2XF DP 1612 B2XF DP 1646 B2XF DP 1614 B2XF PX4A57W3FE ST 4848GLT ST 4949GLT CPS B2XF NG3406 B2XF ST 5020GLT PHY 444 WRF NG 4545 B2XF DP 1518 B2XF PX4A62W3FE PX3A96W3FE PHY 312 WRF ST 4946GLB PX3A99W3FE DG 3109 B2XF NG3522 B2XF DP 1835 B3XF DP 1823 NR B2XF PHY 300 W3FE CROPLAN 9608 B3XF DP 1639 B2XF AMX 1715 B2XF PHY 340 W3FE PX3A82W3FE AMX 1711 B2XF PHY 330 W3FE PX4A52W3FE PHY 490 W3FE PHY 450 W3FE PX2A28W3FE Men LSD ns C.V.% R 2 x UI = Fiber length uniformity Index. 19

22 AAES Reserch Series 650 Tble 14. Yield nd relted properties 2017 Ark. Cotton Vriety Test, with irrigtion on Hebert silt lom t Rohwer. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. PX4A57W3FE PX4A52W3FE PHY 444 WRF DP 1646 B2XF PHY 312 WRF PX4A62W3FE DP 1522 B2XF PHY 300 W3FE PX3A99W3FE PHY 340 W3FE PX4A54W3FE DP 1639 B2XF NG3522 B2XF PHY 330 W3FE DP 1835 B3XF PX3A82W3FE DG 3214 B2XF CROPL B3XF DP 1823 NR B2XF DG 3385 B2XF NG 4601 B2XF DP 1614 B2XF DP 1725 B2XF PHY 490 W3FE DP 1612 B2XF PX3A96W3FE ST 4946GLB ST 5020GLT NG 4545 B2XF DP 1518 B2XF DG 3109 B2XF AMX 1715 B2XF PHY 450 W3FE ST 4949GLT NG3406 B2XF CPS B2XF AMX 1711 B2XF PX2A28W3FE DP 1820 B3XF NG 3517 B2XF ST 4848GLT Men LSD ns C.V.% R 2 x

23 Arknss Cotton Vriety Test 2017 Tble 15. Fiber properties 2017 Arknss Trnsgenic Cotton Vriety Test, with irrigtion on Hebert silt lom t Rohwer. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % PX4A57W3FE PX4A52W3FE PHY 444 WRF DP 1646 B2XF PHY 312 WRF PX4A62W3FE DP 1522 B2XF PHY 300 W3FE PX3A99W3FE PHY 340 W3FE PX4A54W3FE DP 1639 B2XF NG3522 B2XF PHY 330 W3FE DP 1835 B3XF PX3A82W3FE DG 3214 B2XF CROPLAN 9608 B3XF DP 1823 NR B2XF DG 3385 B2XF NG 4601 B2XF DP 1614 B2XF DP 1725 B2XF PHY 490 W3FE DP 1612 B2XF PX3A96W3FE ST 4946GLB ST 5020GLT NG 4545 B2XF DP 1518 B2XF DG 3109 B2XF AMX 1715 B2XF PHY 450 W3FE ST 4949GLT NG3406 B2XF CPS B2XF AMX 1711 B2XF PX2A28W3FE DP 1820 B3XF NG 3517 B2XF ST 4848GLT Men LSD ns C.V.% R 2 x UI = Fiber length uniformity Index. 21

24 AAES Reserch Series 650 Tble 16. Morphologicl nd host-plnt resistnce trits in the 2017 Arknss Cotton Vriety Test. Lef Stem Brct Trnished plnt Vert. Bct. Vriety pub. r pub. r trichomes b r bug dmge c r wilt d r blight e rting rting no./cm % dm. flowers % % sus. AMX 1711 B2XF AMX 1715 B2XF CPS B2XF DG 3214 B2XF CROPL B3XF DG 3109 B2XF DG 3385 B2XF DP 1518 B2XF DP 1522 B2XF DP 1612 B2XF DP 1614 B2XF DP 1639 B2XF DP 1646 B2XF DP 1725 B2XF DP 1820 B3XF DP 1823 NR B2XF DP 1835 B3XF NG 3517 B2XF NG 4545 B2XF NG 4601 B2XF NG3406 B2XF NG3522 B2XF PHY 300 W3FE PHY 312 WRF PHY 330 W3FE PHY 340 W3FE PHY 444 WRF PHY 450 W3FE PHY 490 W3FE PX2A28W3FE PX3A82W3FE PX3A96W3FE PX3A99W3FE PX4A52W3FE PX4A54W3FE PX4A57W3FE PX4A62W3FE ST 4848GLT ST 4946GLB ST 4949GLT ST 5020GLT Ark 0628fg RF (sus.) Ark 0628fg RF (sus.) Ark 0628fg RF (sus.) Men LSD C.V.% R 2 x Lef nd stem pubescence (pub.) rted t Keiser irrigted test (6 plnts per plots, 6 reps) using scle of 1 (smooth lef) to 9 (pilose, very hiry). b Mrginl trichome density of brcts determined on 6 brcts/plot (4 reps) t Keiser irrigted test. c Response to trnished plnt bug ws determined by exmining white flowers (6 flowers/plot/dy for 6 dys) for presence of nther dmge (dm.). Plots were 1-row, replicted 8 times. d Vert. wilt. = Verticillium wilt. Percentge of plnts showing Verticillium wilt symptoms in ech plot ws visully estimted on 28 Aug t Judd Hill. e Bct. blight = bcteril blight. Vrieties/breeding lines were plnted in flts (2 replictions, 13 seed/plot) in greenhouse, nd scrtch inoculted with Xnthomons citris pv. mlvcerum. The inoculum ws obtined from nturlly infected leves collected t the 2017 Keiser loction. Sctches were exmined for wter-soking, nd percentge of susceptible (sus.) plnts ws determined. 22

25 Arknss Cotton Vriety Test 2017 Tble 17. Two-yer nd three-yer verge lint yields (lb/a) for trnsgenic vrieties t the five loctions of the Arknss Cotton Vriety Test. Mnil Keiser Judd Hill Mrinn Rohwer All Vriety Trits Irrigted Irrigted r Irrigted r Irrigted r Irrigted r loctions r lb/a lb/a lb/a lb/a lb/a lb/a Two-yer ( ) mens DP 1646 B2XF B2XF PHY 444 WRF WRF PHY 312 WRF WRF DP 1522 B2XF B2XF ST 4946GLB2 GLB DG 3385 B2XF B2XF vf NG3522 B2XF B2XF DP 1725 B2XF B2XF DP 1639 B2XF B2XF DP 1518 B2XF B2XF ST 4949GLT GLT DP 1612 B2XF B2XF DP 1614 B2XF B2XF NG3406 B2XF B2XF ST 4848GLT GLT Men Three-yer ( ) mens DP 1646 B2XF B2XF PHY 312 WRF WRF PHY 444 WRF WRF DP 1522 B2XF B2XF DP 1518 B2XF B2XF DG 3385 B2XF B2XF ST 4946GLB2 GLB NG3522 B2XF B2XF DP 1612 B2XF B2XF DP 1614 B2XF B2XF DP 1639 B2XF B2XF NG3406 B2XF B2XF Men

26 AAES Reserch Series 650 Tble 18. Yield nd relted properties 2017 Arknss Cotton Vriety Test cross four test sites. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. Ark Ark ne Ark Ark SSG UA Ark UA SSG UA UA Ark AM UA BRS BRS SSG HQ 210CT BRS AT Men Vr. LSD Loc. LSD C.V.% R 2 x Prob (vr x loc) < Tble 19. Fiber properties 2017 Arknss Conventionl Cotton Vriety Test cross four test sites. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % Ark Ark ne Ark Ark SSG UA Ark UA SSG UA UA Ark AM UA BRS BRS SSG HQ 210CT BRS AT Men Vr. LSD Loc. LSD ns C.V.% R 2 x Prob (vr x loc) UI = Fiber length uniformity Index. 24

27 Arknss Cotton Vriety Test 2017 Tble 20. Yield nd relted properties 2017 Arknss Cotton Vriety Test, with irrigtion on Shrkey cly soil t Keiser. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. Ark Ark ne Ark Ark SSG UA Ark UA SSG UA UA Ark AM UA BRS BRS SSG HQ 210CT BRS AT Men Vr. LSD Loc. LSD C.V.% R 2 x Prob (vr x loc) < Tble 21. Fiber properties 2017 Arknss Conventionl Cotton Vriety Test, with irrigtion on Shrkey cly soil t Keiser. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % SSG UA Ark Ark ne Ark Ark AM UA UA Ark BRS Ark SSG HQ 210CT SSG UA UA AT BRS BRS Men LSD C.V.% R 2 x UI = Fiber length uniformity Index. 25

28 AAES Reserch Series 650 Tble 22. Yield nd relted properties 2017 Arknss Cotton Vriety Test, with irrigtion on Dundee silt lom soil t Judd Hill. Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. Ark Ark Ark SSG HQ 210CT Ark ne BRS AM UA UA SSG UA Ark BRS Ark BRS SSG UA UA AT Men LSD ns C.V.% R 2 x Tble 23. Fiber properties 2017 Arknss Conventionl Cotton Vriety Test, with irrigtion on Dundee silt lom soil t Judd Hill. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % Ark Ark Ark SSG HQ 210CT Ark ne BRS AM UA UA SSG UA Ark BRS Ark BRS SSG UA UA AT Men LSD ns 0.03 ns C.V.% R 2 x UI = Fiber length uniformity Index. 26

29 Arknss Cotton Vriety Test 2017 Tble 24. Yield nd relted properties 2017 Arknss Cotton Vriety Test, with irrigtion on Cllowy silt lom soil t Mrinn. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. Ark Ark Ark ne UA Ark Ark UA Ark BRS AM UA SSG UA SSG HQ 210CT SSG UA AT BRS BRS Men LSD C.V.% R 2 x Tble 25. Fiber properties 2017 Arknss Conventionl Cotton Vriety Test, with irrigtion on Cllowy silt lom soil t Mrinn. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % Ark Ark Ark ne UA Ark Ark UA Ark BRS AM UA SSG UA SSG HQ 210CT SSG UA AT BRS BRS Men LSD C.V.% R 2 x UI = Fiber length uniformity Index. 27

30 AAES Reserch Series 650 Tble 26. Yield nd relted properties 2017 Arknss Cotton Vriety Test, with irrigtion on Hebert silt lom t Rohwer. Lint Lint Open Seed Lint Seed/ Fibers/ Fiber Vriety yield r frc. r Ht. r bolls r index r index r cre r seed r density r lb/a % cm % g g mil. no. no. Ark ne Ark SSG UA Ark SSG UA Ark Ark UA UA AM UA Ark BRS SSG HQ 210CT BRS AT BRS Men LSD C.V.% R 2 x Tble 27. Fiber properties 2017 Arknss Conventionl Cotton Vriety Test, with irrigtion on Hebert silt lom t Rohwer. Lint Qulity Fiber properties Vriety yield r score r Micronire r Length r UI r Strength r Elongtion r lb/a in. % g/tex % Ark ne Ark SSG UA Ark SSG UA Ark Ark UA UA AM UA Ark BRS SSG HQ 210CT BRS AT BRS Men LSD C.V.% R 2 x UI = Fiber length uniformity Index. 28

31 Arknss Cotton Vriety Test 2017 Tble 28. Morphologicl nd host-plnt resistnce trits in the 2017 Arknss Cotton Vriety Test. Lef Stem Brct Trnished plnt Vert. Bcteril Vriety pub. r pub. r trichomes b r bug dmge c r wilt d r blight e % sus. AM UA Ark ne Ark Ark Ark Ark Ark AT BRS BRS BRS SSG HQ 210CT SSG UA SSG UA UA UA Ark 0628fg RF (sus.) Ark 0628fg RF (sus.) Men LSD C.V.% R 2 x Lef nd stem pubescence (pub.) rted t Keiser irrigted test (6 plnts per plots, 6 reps) using scle of 1 (smooth lef) to 9 (pilose, very hiry). b Mrginl trichome density of brcts determined on 6 brcts/plot (4 reps) t Keiser irrigted test. c Response to trnished plnt bug ws determined by exmining white flowers (6 flowers/plot/dy for 6 dys) for presence of nther dmge. Plots were 1- row, replicted 8 times. d Vert. wilt. = Verticillium wilt. Percentge of plnts showing Verticillium wilt symptoms in ech plot ws visully estimted on 28 Aug t Judd Hill. e Vrieties/breeding lines were plnted in flts (4 replictions, 13 seed/plot) in greenhouse, nd scrtch inoculted with Xnthomons citri pv. mlvcerum. The inoculum ws obtined from nturlly infected leves collected t the 2017 Keiser loction. Sctches were exmined for wter-soking, nd percentge of susceptible (sus.) plnts ws determined. 29

32 AAES Reserch Series 650 Tble 29. Two-yer nd three-yer verge lint yields (lb/a) for conventionl vrieties t the four loctions of the Arknss Cotton Vriety Test. Keiser Judd Hill Mrinn Rohwer All Vriety Trits Irrigted r Irrigted r Irrigted r Irrigted r loctions r lb/a lb/a lb/a lb/a lb/a Two-yer ( ) mens SGS UA107 conv SGS UA222 conv SGS UA114 conv AM UA48 conv SGS UA103 conv BRS conv SGS HQ210CT conv BRS conv BRS conv Men Three-yer ( ) mens SGS UA222 conv AM UA48 conv SGS UA103 conv BRS conv SGS HQ210CT conv BRS conv BRS conv Men

33 Arknss Cotton Vriety Test 2017 Appendix Tble A1. Lint yield nd fiber properties Lee county conventionl vriety test. Coopertor: Soil Type: Nthn Reed Commerce Silt Lom Dte Plnted: Dte of Hrvest: 5/16/17 11/10/17 Irrigtion: Furrow Replictions: 4 Agent: Stn Bker Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex UA DP 1518 B2XF Alltex LA NG 1511 B2RF PCG NG 4601 B2XF NG 3522 B2XF HQ HQ Alltex Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 31

34 AAES Reserch Series 650 Appendix Tble A2. Lint yield nd fiber properties Ashley county trnsgenic vriety test. Coopertor: Soil Type: Bruce Bond Hebert Silt Lom Dte Plnted: Dte of Hrvest: 5/8/17 11/6/17 Irrigtion: Furrow Replictions: 4 Agent: Kevin Norton Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1725 B2XF DP 1646 B2XF NG 3522 B2XF PHY 340 W3FE DG 3385 B2XF ST 4949 GLT NG 4601 B2XF DP 1518 B2XF ST 5020 GLT DG 3109 B2XF PHY 450 W3FE Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 32

35 Arknss Cotton Vriety Test 2017 Appendix Tble A3. Lint yield nd fiber properties Crighed county trnsgenic vriety test. Coopertor: Soil Type: John Johnson Fountin Silt Lom Dte Plnted: Dte of Hrvest: 5/8/17 10/20/17 Irrigtion: Furrow Replictions: 3 Agent: Brnon Thiesse Chris Grimes Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1518 B2XF PHY 340 W3FE PHY 330 W3FE DG 3385 B2XF DP 1646 B2XF DP 1725 B2XF ST 4949 GLT ST 5020 GLT DG 3109 B2XF NG 3522 B2XF NG 4601 B2XF Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 33

36 AAES Reserch Series 650 Appendix Tble A4. Lint yield nd fiber properties Lee county trnsgenic vriety test. Coopertor: Soil Type: Rmey Stiles Loring Silt Lom Dte Plnted: Dte of Hrvest: 5/11/17 10/18/17 Irrigtion: Furrow Replictions: 4 Agent: Stn Bker Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1646 B2XF DP 1518 B2XF DG 3385 B2XF DP 1725 B2XF NG 3522 B2XF ST 5020 GLT DG 3109 B2XF ST 4949 GLT PHY 340 W3FE NG 4601 B2XF PHY 450 W3FE Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 34

37 Arknss Cotton Vriety Test 2017 Appendix Tble A5. Lint yield nd fiber properties Poinsett county trnsgenic vriety test. Coopertor: Soil Type: Mrty White/Jesse Flye Hyti Soil Dte Plnted: Dte of Hrvest: 5/16/17 10/26/17 Irrigtion: Furrow Replictions: 4 Agent: Crig Allen Justin Chlpeck Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1725 B2XF DG 3385 B2XF DG 3109 B2XF NG 3522 B2XF DP 1518 B2XF NG 4545 B2XF NG 3517 B2XF NG 4601 B2XF Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 35

38 AAES Reserch Series 650 Appendix Tble A6. Lint yield nd fiber properties St Frncis county trnsgenic vriety test. Coopertor: Soil Type: Joe Whittenton Loring Silt Lom Dte Plnted: Dte of Hrvest: 5/22/17 10/31/17 Irrigtion: Pivot Replictions: 4 Agent: Cody Griffin Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1725 B2XF PHY 340 W3FE DP 1646 B2XF DP 1518 B2XF PHY 330 W3FE ST 4949 GLT NG 3522 B2XF DG 3385 B2XF DG 3109 B2XF NG 4601 B2XF ST 5020 GLT Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 36

39 Arknss Cotton Vriety Test 2017 Appendix Tble A7. Lint yield nd fiber properties Desh county trnsgenic vriety test. Coopertor: Soil Type: Phillip Morris Rill Silt Lom Dte Plnted: Dte of Hrvest: 5/10/17 10/16/17 Irrigtion: Furrow Replictions: 3 Agent: Chuck Cpps Steve Kelley Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex NG 3522 B2XF DP 1518 B2XF DP 1518 B2XF DP 1646 B2XF DP 1725 B2XF DG 3385 B2XF DG 3109 B2XF NG 4601 B2XF Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 37

40 AAES Reserch Series 650 Appendix Tble A8. Lint yield nd fiber properties Lonoke county trnsgenic vriety test. Coopertor: Soil Type: Rick Brnsford Hebert Silt Lom Dte Plnted: Dte of Hrvest: 5/17/17 10/19/17 Irrigtion: Furrow Replictions: 3 Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1518 B2XF DP 1725 B2XF DP 1646 B2XF DP 1614 B2XF NG 4601 B2XF DG 3385 B2XF DG 3109 B2XF NG 3522 B2XF Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 38

41 Arknss Cotton Vriety Test 2017 Appendix Tble A9. Lint yield nd fiber properties Mississippi county trnsgenic vriety test. Coopertor: Soil Type: Json Bennett Dundee Silt Lom Dte Plnted: Dte of Hrvest: 5/25/17 11/21/17 Irrigtion: Furrow Replictions: 2 Agent: Ry Benson Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1646 B2XF NG 3522 B2XF DG 3385 B2XF DP 1725 B2XF DP 1518 B2XF DG 3109 B2XF NG 4601 B2XF Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 39

42 AAES Reserch Series 650 Appendix Tble A10. Lint yield nd fiber properties Mississippi county trnsgenic vriety test. Coopertor: Soil Type: Dvid Wildy Routon-Dundee- Dte Plnted: Dte of Hrvest: 5/27/17 11/9/17 Crevsse Complex Irrigtion: Furrow Replictions: 4 Agent: Ry Benson Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. % g/tex DP 1725 B2XF DG 3385 B2XF DP 1518 B2XF DG 3109 B2XF ST 4949 GLT DP 1614 B2XF DP 1646 B2XF NG 3522 B2XF PHY 340 W3FE NG 4601 B2XF PHY 330 W3FE ST 5020 GLT Men Vr. LSD C.V.% Prob (vr) UI = Fiber length uniformity index. 40

43 Arknss Cotton Vriety Test 2017 Appendix Tble A11. Lint yield nd fiber properties Cly county trnsgenic vriety test. Coopertor: Soil Type: Dvid Cgle Fountin Silt Lom Dte Plnted: Dte of Hrvest: 5/8/17 As hrvest redy Irrigtion: Furrow Replictions: 1 Agent: Allison Howell Lint Fiber properties Vriety yield r Micronire r Length r UI r Strength r lb/a in. g/tex ST 5020 GLT DP 1725 B2XF DG 3385 B2XF PHY 300 W3FE PHY 340 W3FE NG 3522 B2XF DP 1518 B2XF NG 4601 B2XF DG 3445 B2XF Men UI = Fiber length uniformity index. 41

44 COTTON VARIETY TEST LOCATIONS JHCRS MACR NEREC LMCRS RRS JHCRS - Judd Hill Coopertive Reserch Sttion, ner Trumnn LMCRS - Lon Mnn Cotton Reserch Sttion, Mrinn MACR - Mnil Airport Cotton Reserch Frm, Mnil NEREC - Northest Reserch nd Extension Center, Keiser RRS - Rohwer Reserch Sttion, Rohwer

45

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