GUIDE Learn how K9 Advantix II can help your clinic

Transcription

1 CLINIC GUIDE Learn how K9 Advantix II can help your clinic Do not use on cats.

2 STUDIES & COMPARISONS CLINIC SUPPORT BROAD SPECTRUM SPEED OF KILL REPELLENCY

3 K9 Advantix II offers your patients broad-spectrum protection against fleas, ticks and mosquitoes too. What do you expect from an external parasite control product for dogs? In your practice, you re always looking for quality external parasite control options for your clients and their dogs. The ideal product should offer: Ease of application Convenience Quality manufacturing from a company you trust Reliable efficacy Broad-spectrum protection Repellency Why recommend K9 Advantix II? REPEL-and-kill protection Convenient and effective control of fleas, ticks and mosquitoes if fleas, ticks and mosquitoes are repelled or killed, they cannot bite or attach and transmit the organisms that may cause disease Broad-spectrum activity Achieved through an exclusive combination of three active ingredients: imidacloprid, permethrin and pyriproxyfen What is the speed-of-kill of K9 Advantix II? Starts killing reinfesting ticks within 0 minutes* Kills reinfesting fleas within two hours How can K9 Advantix II help your clinic? Bayer supports your clinic by offering point-of-purchase materials as well as valuable strategies like our Free Tubes Program exclusively for veterinary clinics. *Three days post-treatment on previously non-treated dogs.,2 Dryden M, Payne P, Smith V, et al. (2006). Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Ixodes scapularis and Amblyomma americanum ticks on dogs. Vet Ther. 7(3): Dryden M, Payne P, Smith V, et al. (2006). Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Rhipicephalus sanguineus and Dermacentor variabilis ticks on dogs. Vet Ther. 7(3):87-98.

4 Features & benefits K9 Advantix II provides your clients with all these features and benefits in every tube: TICKS REPELS and kills fleas, ticks and mosquitoes too For use on dogs and puppies 7 weeks of age and older Lasts for one month Proven efficacy Convenient, once-a-month topical application Easy to apply Waterproof Repels and kills ticks, including the four major species: deer ticks, American dog ticks, brown dog ticks and Lone Star ticks Kills ticks that may attach and transmit the organisms that may cause disease such as ehrlichiosis, Lyme disease, anaplasmosis and Rocky Mountain spotted fever Starts killing ticks within 0 minutes* STARTS KILLING TICKS 0 MINUTES* WITHIN * Three days post-treatment on previously non-treated dogs.,2 FLEAS Kills ALL life stages of fleas Prevents fleas on a treated dog from infesting the home Kills fleas within 2 hours, and kills reinfesting fleas within 2 hours MOSQUITOES, BITING FLIES AND LICE REPELS and kills mosquitoes, often before they have a chance to take a blood meal REPELS biting flies Kills lice CONVENIENT, ONCE-A-MONTH TOPICAL APPLICATION K9 Advantix II is waterproof. *Three days post-treatment on previously non-treated dogs., 2 We recommend use of soap-free shampoo. 2

5 WATERPROOF Waterproof protection for dogs With K9 Advantix II, there is no need to reapply after a monthly bath or weekly swimming. A study has shown that the combination of imidacloprid and permethrin will continue to be effective after exposure to water whether your dog has gone for a swim, has been given a bath or has been in the rain. 3 This waterproof protection can be used on puppies as young as seven weeks of age. Consider recommending a soap-free shampoo and towel dry before monthly application. Imidacloprid is distributed in the skin s water-resistant lipid layer, the hair follicles and the sebaceous glands. 4 Water-resistant lipid layer Epidermis Dermis Sebaceous gland Hair follicle Imidacloprid Subcutaneous layer Dryden M, Payne P, Smith V, et al. (2006). Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Ixodes scapularis and Amblyomma americanum ticks on dogs. Vet Ther. 7(3): Dryden M, Payne P, Smith V, et al. (2006). Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Rhipicephalus sanguineus and Dermacentor variabilis ticks on dogs. Vet Ther. 7(3): Data on file. Bayer HealthCare Animal Health. 4 Chopade H, Eigenberg D, Solon E, et al. (200). Skin distribution of imidacloprid by microautoradiography after topical administration to beagle dogs. Vet Ther. Winter;(4):E-E0. 23

6 The power of repellency What makes repellency so important? If a flea, tick or mosquito is repelled or killed, it cannot bite or attach and transmit the organisms that may cause disease. Your clients need to know that not all products repel and kill. Vector-borne diseases are mostly transmitted once a parasite bites or attaches to the host. Even if the parasite dies later on, transmission may have already occurred, and the disease may still be spread to the host dog. That s why repelling or killing parasites is so crucial in a pet owner s approach to caring for their dog s overall health. What does K9 Advantix II repel? K9 Advantix II REPELS and kills fleas, ticks and mosquitoes too. Why recommend K9 Advantix II? K9 Advantix II offers broad-spectrum protection against fleas, ticks and mosquitoes by incorporating imidacloprid, permethrin and pyriproxyfen to create a repel-and-kill mode of action. K9 Advantix II offers a preventive approach to pest protection. Fleas Ticks Mosquitoes K9 Advantix II vs the Competition NexGard (afoxolaner) Chewables Frontline Plus Bravecto (fluralaner) TICKS ARE THE # Topical Systemic Topical Systemic Fleas Repels AND kills Kills Kills Kills Ticks Repels AND kills Kills Kills Kills Mosquitoes Repels AND kills No label claim No label claim No label claim Based on product labels K9 Advantix II REPELS and kills fleas, ticks and mosquitoes too. If fleas, ticks and mosquitoes are repelled or killed, they cannot bite or attach and transmit the organisms that may cause disease. CARRIER OF CANINE VECTOR-BORNE DISEASE. The longer an infected tick remains attached to a dog, the greater the risk of transmitting a disease agent. 4 Rosenthal M. (2008). Vector-borne disease important area of research; ticks most common vector. Vet Forum. 25(2): Anderson FJ, Magnarelli LA. (2008). Biology of ticks. Infect Dis Clin North Am. 22(2): Ribeiro JM, Makoul GT, Levine J, et al. (985). Antihemostatic, anti-inflammatory, and immunosuppressive properties of the saliva of a tick, Ixodes dammini. J Exp Med. 6(2):

7 How diseases can be transmitted The questing tick finds a dog, selects a feeding site and prepares to penetrate the skin with its mouthparts DAYS 2 is how long an adult tick can feed on a dog, if left undisturbed. The saliva of most ticks has substances that can prevent their hosts from recognizing they have attached. 3 Tick saliva facilitates feeding by acting as a local anesthetic, an anticoagulant and a medium for the transfer of disease agents. 3 4 Learn more The tick, if uninterrupted, may feed for several days on the dog s blood, sharing whatever bacteria and parasites it may be carrying. The fully fed (replete) female tick will now detach and drop into the environment, ready to lay thousands of eggs and potentially leaving behind, in the dog, agents that may result in diseases such as Lyme disease, ehrlichiosis, anaplasmosis and Rocky Mountain spotted fever. The female tick may also pass parasites or infectious agents to her offspring through transovarial transmission. 5

8 The power of repellency (continued) Common tick-transmitted diseases: Lyme disease Anaplasmosis Rocky Mountain spotted fever Ehrlichiosis Ticks are the # carrier of Canine Vector-Borne Diseases Learn more (CVBDs) in the US When an infected parasite (such as a tick, flea or mosquito) attaches or bites and feeds on the blood of a dog, it can pass the pathogen from itself to the new host. Ticks are competent vectors because they feed on several hosts, molt through different life stages, require large blood meals, and feed for long periods of time. General transmission times of common canine tick-borne diseases Pathogen Feeding time to infect* Recognized and/or potential tick vectors Anaplasmosis Anaplasma spp. 4 to 48 hours 2 Ixodes scapularis (deer tick) Rhipicephalus sanguineus (brown dog tick) Ixodes pacificus (western black-legged tick) Rocky Mountain spotted fever Rickettsia rickettsii 4 to 48 hours 2 Dermacentor andersoni (Rocky Mountain wood tick) Rhipicephalus sanguineus (brown dog tick) Dermacentor variabilis (American dog tick) Amblyomma americanum (Lone Star tick) Ehrlichiosis Ehrlichia spp. 3 hours 3 Dermacentor variabilis (American dog tick) Rhipicephalus sanguineus (brown dog tick) Amblyomma americanum (Lone Star tick) Lyme disease Borrelia burgdorferi 24 to >48 hours 4 Ixodes scapularis (deer tick) Ixodes pacificus (western black-legged tick) *Some studies performed in non-canine hosts. Transmission time may also vary with the physiologic state of individual ticks P L AT I N U M S p o n s o r 6 For more information about canine vector-borne diseases, visit capcvet.org. Rosenthal M. (2008). Vector-borne disease important area of research; ticks most common vector. Vet Forum. 25(2): Nicholson WL, Allen KE, McQuiston JH, et al. (200). The increasing recognition of rickettsial pathogens in dogs and people. Trends Parasitol. 26(4): Fourie JJ, Stanneck D, Luus HG, et al. (203). Transmission of Ehrlichia canis by Rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes. Vet Parasitol. 97(203): Little SE. From attachment to disease: Diversity in pathogen transmission by ticks. Talk to your Bayer rep today about recommending the power of repellency for your patients.

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10 The power of repellency (continued) K9 Advantix II and pathogen transmission interruption: The following studies support imidacloprid + permethrin, two of the active ingredients in K9 Advantix II. By effectively repelling and killing I. scapularis (deer tick), K9 Advantix II may help reduce the risk of Borrelia burgdorferi (Lyme disease) transmission*,2 Two separate studies differing only by tick challenge study days (day 7 vs. day 25) Each study contained one group of 8 dogs treated on day 0 with imidacloprid and permethrin, and one control group of 8 dogs All dogs were infested with I. scapularis (deer tick) on study day 7 or study day 25. Ticks remained in place for 7 days in the first study and 5 days in the second study 0 ml of whole blood drawn on study days 4, 25, 42, 56, 70, and 9 in the day 7 challenge protocol, and study days 42, 56, 70, 90, and 0 in the day 25 challenge protocol Lyme disease may be transmitted as fast as 24 hours after attachment. 4 Studies performed in non-canine hosts. Transmission time may also vary with the physiologic state of individual ticks. Group A (treated once with imidacloprid and permethrin) Group B (not treated) Number of dogs testing positive by kelisa for B. burgdorferi in the day 7 challenge study 0 out of 8 8 out of 8 % positive dogs 0% 00% Number of dogs testing positive by kelisa for B. burgdorferi in the day 25 challenge study 0 out of 8 7 out of 8 According to the Companion Animal Parasite Council, of the dogs tested for Lyme disease, in 6 dogs in the US tested positive for Lyme disease.** 3 ** Only tests reported by two national laboratories. % positive dogs 0% 88% In the above studies, by repelling and killing ticks, K9 Advantix II* effectively prevented the transmission of the organisms that can cause Lyme disease. *Studies were performed with imidacloprid and permethrin (two of the active ingredients in K9 Advantix II). 203 CAPC STATS : 34,749 dogs tested positive for Lyme disease across 50 states 3 63,854 dogs tested positive for Ehrlichiosis across 50 states 3 68,286 dogs tested positive for Anaplasmosis across 50 states 3 The numbers for each disease represent the total number of positive dogs out of all dogs tested at only two national laboratories. Therefore, the true prevalence may be higher. A recent publication showed that ticks may be able to transmit certain disease agents to dogs in as little as 5 3 hours. Spencer JA, Butler JM, Stafford KC, et al. (2003). Evaluation of permethrin and imidacloprid for prevention of Borrelia burgdorferi transmission from blacklegged ticks (Ixodes scapularis) to Borrelia burgdorferi-free dogs. Parasitol Res. 90(Suppl 3):S06-S07. 2 Blagburn BL, Spencer JA, Butler JM, et al. (2005). Prevention of transmission of Borrelia burgdorferi and Anaplasma phagocytophilum from ticks to dogs using K9 Advantix and Frontline Plus applied 25 days before exposure to infected ticks. Intern J Appl Res Vet Med. 3(2): Parasite Prevalence Maps. CAPC website. Available at: Accessed 0/24/ Little SE. From attachment to disease: Diversity in pathogen transmission by ticks. little-attachment.shtml. 5 Fourie JJ, Stanneck D, Luus HG, et al. (203). Transmission of Ehrlichia canis by Rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes. Vet Parasitol. 97(203): Otranto D, Paradies P, Testini G, et al. (2008). Application of 0% imidacloprid/50% permethrin to prevent Ehrlichia canis exposure in dogs under natural conditions. Vet Parasitol. 53(3-4): Blagburn BL, Spencer JA, Billeter SA, et al. (2004). Use of imidacloprid-permethrin to prevent transmission of Anaplasma phagocytophilum from naturally infected Ixodes scapularis ticks to dogs. Vet Ther. 5(3):22-27.

11 By effectively repelling and killing R. sanguineus (brown dog tick), K9 Advantix II may help reduce the risk of Ehrlichia canis (ehrlichiosis) transmission under field conditions* dogs enrolled in a year-long trial. Study was conducted in field conditions following Good Clinical Practice (GCP) guidelines. Dogs were treated from April to November Dogs were housed in an environment with R. sanguineus (brown dog tick) The protection efficacy was determined by serologic testing Indirect Fluorescent Antibody Test (IFAT) Reduced rate of transmission of E. canis observed in the study* 6 Group A (treated once a month with imidacloprid and permethrin) Group B (treated twice a month with imidacloprid and permethrin) % of protection 00% 95.57% According to the Companion Animal Parasite Council, of the dogs tested for ehrlichiosis, in 33 dogs in the US tested positive, and of the dogs tested for anaplasmosis, in 3 dogs in the US tested positive. 3 Only tests reported by two national laboratories. Group A (treated once a month with imidacloprid and permethrin) Group B (treated twice a month with imidacloprid and permethrin) In the above study, by repelling and killing ticks, K9 Advantix II* effectively reduced the transmission of organisms that can cause ehrlichiosis. *Study performed with imidacloprid and permethrin (two of the active ingredients in K9 Advantix II). Group C (not treated) Number E. canis seropositive dogs at final follow-up 0 25 % E. canis seropositive dogs at final follow-up 0.00% 0.6% 5.3% By effectively repelling and killing I. scapularis (deer tick), K9 Advantix II may help reduce the risk of Anaplasma phagocytophilum transmission 2,7 Each study contained one group of 8 dogs treated on day 0 with imidacloprid and permethrin, and one control group of 8 dogs All dogs were infested with I. scapularis (deer tick) on study day 7 or study day 25 0 ml of whole blood drawn on study day 90 in the day 7 challenge protocol, and study day 0 in the day 25 challenge protocol for serologic testing Ticks were removed after 7 days in the 7-day study and 5 days in the 25-day study Reduced rates of transmission of A. phagocytophilum observed in the studies 2,7 Group A (treated once with imidacloprid and permethrin) Group B (not treated) Number of dogs testing positive by IFAT for A. phagocytophilum in the day 7 challenge study 0 out of 8 7 out of 8 % positive dogs on study day 90 0% 88% Number of dogs testing positive by IFAT for A. phagocytophilum in the day 25 challenge study 0 out of 8 7 out of 8 % positive dogs on study day 0 0% 88% In the above studies, by repelling and killing ticks, K9 Advantix II effectively prevented the transmission of the organisms that can cause anaplasmosis. Studies were performed with imidacloprid and permethrin (two of the active ingredients in K9 Advantix II). 9

12 Speed of kill Your clients have a need for speed It doesn t take long for an infected parasite to transmit the pathogens behind such diseases as Lyme disease, Rocky Mountain spotted fever and more. K9 Advantix II kills fleas and ticks quickly* -5 Imidacloprid stops biting fleas within 3 5 minutes.* 3-5 Fleas bites can cause health problems such as: Flea allergy dermatitis Anemia (with severe flea infestations) Fleas are killed within 2 hours of initial application Reinfesting fleas are killed within 2 hours * Three in vivo experiments were conducted to evaluate the effect of Advantage Topical Solution (imidacloprid) on fleas (Ctenocephalies felis). A total of seven (7) dogs were treated with Advantage Topical Solution. Seven (7) days after application, areas on the dogs were shaved to allow visual observation. A petri dish was attached over each spot, and then infested with up to 00 fleas. In all replicates, the fleas stopped their feeding activity and started tetanic trembling movements within 3 5 minutes. In one hour, all fleas were dead. For speed of kill on ticks, day 3 study data demonstrated quick and effective results: 96.4% to 00% of repelled ticks were dead within 0 minutes In this study, 00% tick control was achieved 24 hours after the first infestation STARTS KILLING TICKS 0 MINUTES WITHIN Three days post-treatment on previously non-treated dogs.,2 Day 3 post-treatment study data demonstrated quick and effective results % Repelled % Repelled ticks that died % Tick control 0 min 0 min 3 hr 3 hr 24 hr Amblyomma americanum Lone Star tick 86% 00% 00% 88.6% 00% Ixodes scapularis deer tick 72% 96.4% 00% 92.9% 00% Rhipicephalus sanguineus 2 brown dog tick 83% 00% 00% 00% 00% Dermacentor variabilis 2 American dog tick 93% 99% 00% 00% 00% Immediately after the completion of the 0-minute exposure period, the ticks were found dead or moribund. Imidacloprid and permethrin were applied to dogs on study day 0 25 adults of each tick species were applied to dogs on study day 3 Your clients will appreciate a product that quickly kills fleas and ticks. The longer an infected tick remains attached to a dog, the greater the risk of transmitting a disease agent. 0 Dryden M, Payne P, Smith V, et al. (2006). Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Ixodes scapularis and Amblyomma americanum ticks on dogs. Vet Ther. 7(3): Dryden M, Payne P, Smith V, et al. (2006). Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Rhipicephalus sanguineus and Dermacentor variabilis ticks on dogs. Vet Ther. 7(3): Mehlhorn H, Hansen O, Mencke N. (200). Comparative study on the effects of three insecticides (fipronil, imidacloprid, selamectin) on developmental stages of the cat flea (Ctenocephalides felis Bouché 835); a light and electron microscopic analysis of in vivo and in vitro experiments. Parasitol Res. 87(3): Mehlhorn H. (2000). Mode of action of imidacloprid and comparison with other insecticides (i.e., fipronil and selamectin) during in vivo and in vitro experiments. Suppl Compend Contin Educ Pract Vet. 22(4A): Mehlhorn H, Mencke N, Hansen O. (999). Effects of imidacloprid on adult and larval stages of the flea Ctenocephalides felis after in vivo and in vitro application: a light- and electron-microscopy study. Parasitol Res. 85(8-9):

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14 The value of a broad spectrum What external parasites should your preventive product target? Dog owners want to make sure they re doing everything they can to help their dog have protection against parasites, which is no small task considering the many parasites that a dog can encounter throughout the day. K9 Advantix II REPELS and kills fleas, ticks and mosquitos too, kills ALL life stages of fleas, repels biting flies and kills lice. F LEAS FLEA EGGS F LEA LARVAE TICKS MOSQUITOES BITING FLIES How does K9 Advantix II provide broad-spectrum protection? The combination of imidacloprid and permethrin increases neuronal activity in vitro. Imidacloprid and permethrin attack different receptors on the parasite s nerve, causing an initial excitatory effect and hyperactivity, and then death Pyriproxyfen, an insect growth regulator (IGR), affects the early flea life stages to prevent development to the next stage LICE K9 Advantix II offers your clients broad-spectrum protection against fleas, ticks and mosquitoes too. Neural activity of imidacloprid and permethrin Nervous System Activity Baseline 300 nm Imidacloprid 2 cps 42 cps 500 nm Permethrin 25 cps 500 nm Permethrin 300 nm Imidacloprid 65 cps Each compound acts on a different receptor area of the nerve cell. Imidacloprid: (nicotinic receptors) irreversibly opens nicotinic receptors on postsynaptic neurons, resulting in constant neuromuscular stimulation, spastic paralysis and death Permethrin: (sodium channels) causes prolonged sodium ion exchange across neuronal membranes, resulting in constant nerve discharge followed by spastic paralysis and death 2

15 Activity on Fleas K9 Advantix II effectively breaks the flea life cycle 5% of a flea infestation is visible as adults. The other 95% of an infestation live as immature stages inside the pet s surroundings. 2 In just 30 days, 0 FLEAS can become an infestation of up to 250,000 on your dog and in your home. 4 Adult fleas hatch from the cocoon and continue to infest the pet. 4 FLEA PUPAE 0% of an infestation 2 Flea larvae spin a cocoon and develop into pupae (approximately 2 weeks). 3 ADULT FLEAS 5% of an infestation 2 FLEA LARVAE FLEA CYCLE 3 35% of an infestation 2 Adult fleas lay eggs after a blood meal, which then drop off into the environment. FLEA EGGS 50% of an infestation 2 Flea larvae hatch out of flea eggs when conditions are right (warm and humid). With an IGR as part of your flea control program, the flea life cycle is disrupted at multiple stages, which helps your clients treat and prevent flea infestations. Fleas and flea bites Fleas can aggravate a dog and affect the way he or she bonds with his or her owner. More importantly, fleas may transmit tapeworms or cause skin problems, such as flea allergy dermatitis. 2 Integral parts of integrated flea control include an adulticide to provide the necessary adult flea kill on the dog and an insect growth regulator to suppress the reproduction of surviving adults. M.W. Dryden, DVM, PhD Imidacloprid, an active ingredient in K9 Advantix II, is effective against adult fleas and larval flea stages. In addition to its adulticide flea efficacy, imidacloprid demonstrates a larvicidal flea efficacy when the larvae in the dog s surroundings come into contact with the K9 Advantix IItreated dog. Another benefit of imidacloprid is that fleas do not need to bite the treated animal to be exposed to the active ingredient. K9 Advantix II contains pyriproxyfen, an IGR that contains ovicidal and larvicidal properties to target flea infestations. Methfessel C, Turberg A. (2003). An in vitro demonstration of synergy between imidacloprid and permethrin. Suppl Compend Contin Educ Pract Vet. 25(5A):-4. 2 Grace SF. Fleas. In: Norsworthy GD, Crystal MA, Grace SF, et al., eds. (2006). The Feline Patient. 3rd ed. Ames, IA: Blackwell Publishing; Dryden MW, Payne P, Zurek L. (2003). Pests That Affect Human Health: Fleas Infesting Pets and Homes. Manhattan, KS: Kansas State University Agricultural Experiment Station and Cooperative Extension Service. MF Protecting animals in summer. WAF website. Available at: Accessed July 20,

16 The value of a broad spectrum (continued) Activity on Ticks K9 Advantix II effectively repels and kills the most common tick species. View more supporting studies in the Repellency section. K9 Advantix II repels and kills: Deer ticks (Ixodes scapularis) Male Potential vector of: Anaplasmosis Lyme disease Learn more Male Female American dog ticks (Dermacentor variabilis) Male Female Brown dog ticks (Rhipicephalus sanguineus) Potential vector of: Rocky Mountain spotted fever Ehrlichiosis Potential vector of: Anaplasmosis Rocky Mountain spotted fever Ehrlichiosis Some ticks can survive and reproduce at temperatures as low as 40 F Male Female Lone Star ticks (Amblyomma americanum) Potential vector of: Rocky Mountain spotted fever Ehrlichiosis Male Female Pictures supplied by Kansas State University CVM ITC. M.W. Dryden, DVM, PhD, and P.A. Payne, DVM, PhD,

17 Real-World Field Study The combination of imidacloprid and permethrin is effective against A. americanum (Lone Star tick) 2 The effects of imidacloprid and permethrin on ticks can result in prevention of attachment, inhibition of feeding and rapid death In a natural tick exposure study, the combination of imidacloprid and permethrin demonstrated an efficacy at 48 hours post-exposure (ticks repelled and/or killed) of 93.5% or above from study day 3 through the duration of the study (or day 28) when treated on day 0 Imidacloprid and permethrin were still effective at 28 days Tick repellency 2 to 3 hours after exposure 2 (%) Efficacy 00.0% 90.0% 80.0% 70.0% 60.0% 50.0% 40.0% 30.0% 20.0% 0.0% 00.0% 88.0% 77.5% 68.% 64.3% 68.5% Study day Offer broad-spectrum protection against fleas, ticks, mosquitoes, biting flies and lice. (%) Efficacy 00.0% 90.0% 80.0% 70.0% 60.0% 50.0% 40.0% 30.0% 20.0% 0.0% 00.0% Amblyomma americanum ticks repelled and/or 2 killed 48 hours after exposure 93.5% 98.9% 94.6% 94.% 96.6% Study day Studies were performed with imidacloprid and permethrin (two of the active ingredients in K9 Advantix II). Values at all time points were significant (P <.05) compared to controls. Ogden NH, Lindsay LR, Beauchamp G, et al. (2004). Investigation of relationships between temperature and developmental rates of ticks Ixodes scapularis (Acari: Ixodidae) in the laboratory and field. J Med Entomol. 4(4): Dryden MW, Payne PA, Smith V, et al. (2006). Efficacy of imidacloprid (8.8% w/w) plus permethrin (44% w/w) spot-on topical solution against Amblyomma americanum infesting dogs using a natural tick exposure model. Vet Ther. 7(2):

18 The value of a broad spectrum (continued) Activity on Mosquitoes K9 Advantix II repels and kills mosquitoes for up to four weeks. In addition to year-round protection with a heartworm preventive, the American Heartworm Society recommends the following steps to help reduce pet exposure to mosquitoes : Use approved mosquito repellants/environmental yard controls Keep pets in at night Contact an appropriate agency for mosquito control K9 Advantix II is not labeled for the prevention of heartworm disease and should not eliminate the need for a heartworm preventive product. Mosquitoes can transmit heartworms Once an infected mosquito bites a dog, heartworm larvae may migrate to the bloodstream and eventually invade the lungs and heart. Mosquito bites can lead to stress and skin infection from scratching Mosquitoes are the vector of heartworms They can be a year-round concern in many parts of the country A single dog can be infected with as many as 250 heartworms. It only takes one bite from an infected mosquito 2 Study Support Mosquitoes Kill efficacy against mosquitoes 3 Three dogs in Group were treated with a combination of imidacloprid and permethrin on study day 0; three dogs in Group 2 received a placebo On test days, dogs were placed in mosquito-rearing, screened cages with 50 female mosquitoes After hour, dogs were removed from the cage and mosquitoes were counted and analyzed This study demonstrated that the combination of imidacloprid and permethrin killed up to 98.3% of mosquitoes and was up to 95.2% effective in reduction of blood feeding 3 Kill efficacy against mosquitoes Learn more 88% of dog owners would want their flea and tick product to also protect against mosquitoes.* * Pet owners were recruited from a nationally representative sample of U.S. households. 88% of the 780 participants, who were dog owners who purchased any flea/tick product in the past 2 months, responded that they agreed with the statement that they would want their flea and tick product to protect their dog against mosquito bites. 4 6 Days post-treatment

19 Activity on Biting Flies K9 Advantix II repels and prevents blood-feeding by biting flies. Efficacy against biting flies 5 This study demonstrated that the combination of imidacloprid and permethrin repels (up to 96.9%) and prevents biting flies from blood feeding on dogs. 5 The combination of imidacloprid and permethrin was applied to 8 dogs on study day 0 All dogs were exposed to Stomoxys calcitrans (stable fly) for 30 minutes on study days, 8, 8, 22, and 29 For more information, check the studies section of this guide, or contact your Bayer representative to see how broad-spectrum protection can help your patients. Photo credit: Courtesy of Lynette Cole, DVM, DACVD Activity on Chewing Lice K9 Advantix II kills chewing lice. Efficacy against chewing lice 6 This study demonstrated that the combination of imidacloprid and permethrin is up to 00% effective against chewing lice dogs with natural Trichodectes canis (chewing-louse) infestations: 0 treated with imidacloprid and permethrin; 0 in non-treated control group Imidacloprid and permethrin were applied to treatment group on study day 0 Louse counts were conducted before treatment (study day -) and on study days, 2, 7, 4, 2, 28, and 35 American Heartworm Society. Heartworm Preventive Resistance: Is it Possible? Published December 5, 203. Accessed November 3, Bowman DD, Atkins CE. (2009). Heartworm biology, treatment, and control. Vet Clin North Am Small Anim Pract. 39(6): Data on file. Bayer HealthCare Animal Health. 4 Data on file. Bayer HealthCare Animal Health. 5 Data on file. Bayer HealthCare Animal Health. 6 Stanneck D, Ketzis J, Doyle J, et al. (2008). Lice management options: efficacy of three imidacloprid-containing formulations against natural lice (Trichodectes canis) infestations in dogs. Compend Contin Educ Pract Vet. 30(6B):

20 So protect your furry friend with K9 Advantix II. Its broad-spectrum protection kills fleas, ticks and mosquitoes too. K9 Advantix II is for use on dogs only. 204 Bayer HealthCare LLC, Animal Health Division, Shawnee Mission, Kansas 6620 Bayer (reg d), the Bayer Cross (reg d), K9 Advantix and for the love of dog are trademarks of Bayer. K469 K9 Advantix II supports your clinic s marketing efforts We share your vision At Bayer, we rely on your recommendation of our products and we truly appreciate your advocating our brand. We are committed to supporting veterinarians and their clinics through our incentive programs, including Bayer Partners and the Free Tubes Program. Our new pet kits include a complimentary tube of K9 Advantix II to help initiate the use of a preventive product. We also offer continuing education and symposiums at relevant trade shows, and sponsor several charities that support animal causes. Why carry a trusted brand? The term brand embodies something beyond packaging or trademarks. It s a persona that lives in the minds and sometimes hearts of the consumer. When you offer a certain brand at your clinic, your clients confidence in that brand is strengthened and they may be more inclined to make purchases. Your clients recognize K9 Advantix II From TV commercials to magazine ads to social media, K9 Advantix II has positioned itself as a trusted brand from a well-established company. Loyal customer base 94% of K9 Advantix II users show repeat purchase intent* More than 72% of reviewers on K9AdvantixII.com would recommend K9 Advantix II 2 K9 Advantix II has a total brand awareness of 63% among dog-only households Clients may be likely to ask you for this product by name. More than 72% of reviewers on K9AdvantixII.com would recommend K9 Advantix II. 2 TO YOU, HE S MORE THAN JUST A PET. Scan to watch TV ad Brand loyalty can become clinic loyalty in one visit. Make sure your customers see what they re looking for and keep them coming back for K9 Advantix II. TV ads have been proven to drive viewers to ask their veterinarian about the product. 4 * Pet owners were recruited from a nationally representative sample of U.S. households. Consumers were required to own a dog, be at least 8 years of age, and be the primary decision maker regarding their pet s health or pet products purchased. Current users of K9 Advantix II responded that they definitely will or will purchase K9 Advantix II again the next time they purchase a flea or tick control product for their dog. 8 Pet owners were recruited from a nationally representative sample of U.S. households. Consumers were required to own a dog, be at least 8 years of age, and be the primary decision maker regarding their pet s health or pet products purchased. Pet owners were asked which flea and tick products they could think of or had ever heard of. 3

21 We work to bring clients through your door As always, we re doing our part to reach out to dog owners to increase your foot traffic, and remind pet owners to come back to your clinic. 85% of pet owners purchase flea and/or tick products at the same place they have purchased before.* Therefore it s important for us to remind them to come back to your clinic. Some of our ongoing activities include: and direct mail We reach out to current and potential customers and drive them to purchase K9 Advantix II in veterinary clinics. Promotional materials We create branded signage for you to display at your clinic to generate interest and steer purchase decisions. Reminder cards We offer cards you can send to clients as a reminder that it s time to come in and purchase more product. Free Tubes Program A Bayer-funded program (exclusively for veterinary clinics) that provides competitive discounts on K9 Advantix II. for the for the of dog Broad-spectrum protection against fleas, ticks and mosquitoes too of dog Scan to learn more or visit WhyK9AdvantixII.com K9 Advantix II is for use on dogs only. 204 Bayer HealthCare LLC, Animal Health Division, Shawnee Mission, Kansas 6620 Bayer, the Bayer Cross and K9 Advantix are registered trademarks of Bayer. PS457 Broad-spectrum protection against fleas, ticks and mosquitoes too recommended veterinarians BY K9 Advantix II is for use on dogs only. Ready to receive some special promotions and client educational materials for your clinic? Contact your Bayer rep today. The Free Tubes Program is offered exclusively to veterinary clinics. * 2,500 pet owners were recruited from a nationally representative sample of U.S. households. Consumers were required to own a dog and/or cat and have purchased flea and/or tick products in the last 2 months. Pet owners answered the question I will likely continue to purchase flea and/or tick products from the same source with yes. 5 Data on file. Bayer HealthCare Animal Health. 2 Data on file. Bayer HealthCare Animal Health. 3 Data on file. Bayer HealthCare Animal Health. 4 Data on file. Bayer HealthCare Animal Health. 5 Data on file. Bayer HealthCare Animal Health. 9

22 Studies & comparisons K9 Advantix II competitive profiles NexGard (afoxolaner) Chewables, Bravecto (fluralaner), Frontline Plus, Frontline TriTak and Parastar Plus kill fleas and ticks, but DO NOT REPEL. If a flea or tick is repelled or killed, it cannot bite or attach and transmit organisms that may cause disease. K9 Advantix II vs NexGard (afoxolaner) Chewables K9 Advantix II NexGard (afoxolaner) Chewables Kills Repels Kills Repels Fleas Ticks * Mosquitoes Biting flies Chewing lice K9 Advantix II REPELS and kills fleas, ticks and mosquitoes too. Based on label comparison to K9 Advantix II indications *Dermacentor variabilis, Amblyomma americanum and Ixodes scapularis K9 Advantix II vs Bravecto (fluralaner) K9 Advantix II Bravecto (fluralaner) Kills Repels Kills Repels Fleas Ticks Mosquitoes Biting flies Chewing lice Based on label comparison to K9 Advantix II indications K9 Advantix II works continuously for up to four weeks. Ctenocephalides felis (2-week activity) Ixodes scapularis, Demacentor variabilis, Rhipicephalus sanguineus (2-week activity); Amblyomma americanum (8-week activity) K9 Advantix II kills fleas and ticks through contact with a treated dog. Fleas don t have to bite to die. Ticks don t have to attach to die. K9 Advantix II not only kills but also REPELS fleas, ticks and mosquitoes. Oral products only kill, and the parasite must bite to die. K9 Advantix II works through contact. Fleas and ticks do not need to bite or attach to die. 20

23 K9 Advantix II vs Frontline Plus K9 Advantix II Frontline Plus Kills Repels Kills Repels Fleas Ticks Mosquitoes Biting flies Chewing lice Based on label comparison to K9 Advantix II indications K9 Advantix II vs Frontline TriTak K9 Advantix II Frontline TriTak Kills Repels Kills Repels Fleas Ticks Mosquitoes Biting flies Chewing lice A recent study points out that 60% of surveyed dog owners prefer to use topical flea and tick prevention; 24% would prefer an oral flea and tick product. The remainder use treatment products such as shampoos.* Based on label comparison to K9 Advantix II indications K9 Advantix II vs Parastar Plus K9 Advantix II Parastar Plus Kills Repels Kills Repels Fleas Ticks Mosquitoes Biting flies Chewing lice Based on label comparison to K9 Advantix II indications *Pet owners were recruited from a nationally representative sample of U.S. households. Consumers were required to own a dog, be at least 8 years of age, and be the primary decision maker regarding their pet s health or pet products purchased. Pet owners were asked what type of flea and tick product they would prefer to buy in the future. Data on file. Bayer HealthCare Animal Health. 2

24 Studies & comparisons (continued) K9 Advantix II competitive profile (continued) Activyl Tick Plus repels ONLY TICKS. K9 Advantix II vs Activyl Tick Plus K9 Advantix II Activyl Tick Plus Kills Repels Kills Repels Fleas Ticks Mosquitoes Biting flies Chewing lice Based on label comparison to K9 Advantix II indications K9 Advantix II vs Vectra 3D K9 Advantix II vs Vectra 3D : brand recognition K9 Advantix II is a well-recognized brand that clients may be likely to visit your clinic to buy K9 Advantix II has a total brand awareness of 63% among dog-only households In comparison, Vectra 3D has just 6% recognition* K9 Advantix II has a total brand awareness of 63% among dog-only households * Brand awareness for K9 Advantix II = 63% among dog-only households Vectra 3D = only 6% 22 K9 Advantix II has a small application volume Dog Weight Application Volume 4 0 lbs 0.04 fl oz (0.4 ml) 20 lbs fl oz (.0 ml) 2 55 lbs fl oz (2.5 ml) Over 55 lbs K9 Advantix II 0.35 fl oz (4.0 ml) *Pet owners were recruited from a nationally representative sample of U.S. households. Consumers were required to own a dog, be at least 8 years of age, and be the primary decision maker regarding their pet s health or pet products purchased. Pet owners were asked which flea and tick products they could think of or had ever heard of. Data on file. Bayer HealthCare Animal Health. 40 lb dog Dog Weight Application Volume lbs fl oz (.6 ml) 2 55 lbs 0.22 fl oz (3.6 ml) lbs 0.59 fl oz (4.7 ml) Over 95 lbs 45.2% more volume than K9 Advantix II for one monthly treatment on dogs weighing 2 55 lbs. Vectra 3D fl oz (8.0 ml) Your clinic depends on client satisfaction. Carrying K9 Advantix II can help keep them coming back.

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26 Studies & comparisons (continued) Supporting studies K9 Advantix II is well supported by hundreds of studies Sample of published studies conducted using imidacloprid and/or permethrin: Repel-and/or-kill (efficacy) Cruthers L, Slone R, Arther RG, Davis W, Settje T. (2003). K9 Advantix (imidacloprid plus permethrin) for controlling ticks and mosquitoes on dogs. Suppl Compend Contin Educ Pract Vet. 25:5-8. Dautel H, Kahl O, Siems K, Oppenrieder M, Muller-Kuhrt L, Hilker M. (999). A novel test system for detection of tick repellents. Entomol Exp Appl. 9(3): Dautel H, Turberg A. (2003). The Moving-Object-Bioassay, a laboratory method to determine the repellent efficacy of a permethrin and imidacloprid combination (K9 Advantix). In: Proceedings of the 7th International Symposium on Ectoparasites of Pets; April 3-6, 2003; League City, TX. 38. Dryden MW, Payne PA, Smith V, Hostetler J. (2006). Efficacy of imidacloprid (8.8% w/w) plus permethrin (44% w/w) spot-on topical solution against Amblyomma americanum infesting dogs using a natural tick exposure model. Vet Ther. 7(2): Dryden MW, Payne PA, Smith V, Hostetler J. (2006). Evaluation of an imidacloprid (8.8% w/w) permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w) (S)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Ixodes scapularis and Amblyomma americanum ticks on dogs. Vet Ther. 7(3): Dryden MW, Payne PA, Smith V, Hostetler J. (2006). Evaluation of an imidacloprid (8.8% w/w) permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w) (S)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Rhipicephalus sanguineus and Dermacentor variabilis ticks on dogs. Vet Ther. 7(3): Epe C, Coati N, Stanneck D. (2003). Efficacy of the compound preparation imidacloprid 0%/permethrin 50% spot-on against ticks (I. ricinus, R. sanguineus) and fleas (C. felis) on dogs. Parasitol Res. 90(Suppl 3):S22-S24. Hellmann K, Knoppe T, Krieger K, Stanneck D. (2003). European multicenter field trial on the efficacy and safety of a topical formulation of imidacloprid and permethrin (Advantix) in dogs naturally infested with ticks and/or fleas. Parasitol Res. 90(Suppl 3):S25-S26. Mencke N. (2005). Acaricidal and repellent properties treatment of dogs with imidacloprid 0%/permethrin 50% spot-on solution. In: Proceedings of the 6 th Bayer Ectoparasite Symposium; BSAVA Congress 2005; Birmingham, UK ticks 24

27 fleas mosquitoes Supporting studies (continued) Epe C, Coati N, Stanneck D. (2003). Efficacy of the compound preparation imidacloprid 0%/permethrin 50% spot-on against ticks (I. ricinus, R. sanguineus) and fleas (C. felis) on dogs. Parasitol Res. 90(Suppl 3):S22-S24. Genchi C, Traldi PG, Bianciardi PP. (2000). Efficacy of imidacloprid on dogs and cats with natural infestations of fleas, with special emphasis on flea hypersensitivity. Vet Ther. (2):7-80. Hellmann K, Knoppe T, Krieger K, Stanneck D. (2003). European multicenter field trial on the efficacy and safety of a topical formulation of imidacloprid and permethrin (Advantix) in dogs naturally infested with ticks and/or fleas. Parasitol Res. 90(Suppl 3):S25-S26. Hopkins TJ, Woodley I, Gyr P. (997). Imidacloprid topical formulation: larvicidal effect against Ctenocephalides felis in the surroundings of treated dogs. Suppl Compend Contin Educ Pract Vet. 9(5):4-0. Mehlhorn H, Mencke N, Hansen O. (999). Effects of imidacloprid on adult and larval stages of the flea Ctenocephalides felis after in vivo and in vitro application: a light- and electron-microscopy study. Parasitol Res. 85(8-9): Mehlhorn H. (2000). Mode of action of imidacloprid and comparison with other insecticides (i.e., fipronil and selamectin) during in vivo and in vitro experiments. Suppl Compend Contin Educ Pract Vet. 22(4A):4-8. Rust M, Waggoner M, Mencke N, Hansen O, Vaughn M. (2002). Disruption of adult cat flea feeding by imidacloprid. Suppl Compend Contin Educ Pract Vet. 24(4B):4-5. Cruthers L, Slone R, Arther RG, Davis W, Settje T. (2003). K9 Advantix (imidacloprid plus permethrin) for controlling ticks and mosquitoes on dogs. Suppl Compend Contin Educ Pract Vet. 25(5A):5-8. Hayasaki M, Saeki H. (2009). Inhibition and prevention efficacy against mosquito bloodsucking and Dirofilaria immitis infection by administration of topical insecticide. J Vet Med Sci. 7(8): Machida H, Kondo T, Kanehira K, Hagimori I, Kamio T. (2008). The inhibitory effect of a combination of imidacloprid and permethrin on blood feeding by mosquitoes in dogs raised under outdoor conditions. Vet Parasitol. 54(3-4):

28 Studies & comparisons (continued) Supporting studies (continued) Fourie LJ, Stanneck D, Horak IG. (2006). The efficacy of a topically applied combination of imidacloprid and permethrin against Stomoxys calcitrans on dogs. Intern J Appl Res Vet Med. 4(): Hanssen I, Mencke N, Asskildt H, Ewald-Hamm D, Dorn H. (999). Field study on the insecticidal efficacy of Advantage against natural infestations of dogs with lice. Parasitol Res. 85(4): Stanneck D, Ketzis J, Doyle J, Fisher M. (2008). Lice management options: efficacy of three imidacloprid-containing formulations against natural lice (Trichodectes canis) infestations in dogs. Suppl Compend Contin Educ Vet. 30(6B). biting flies lice Canine vector-borne diseases Blagburn BL, Spencer JA, Billeter SA, et al. (2004). Use of imidacloprid-permethrin to prevent transmission of Anaplasma phagocytophilum from naturally infected Ixodes scapularis ticks to dogs. Vet Ther. 5(3): Otranto D, de Caprariis D, Lia RP, et al. (200). Prevention of endemic canine vector-borne diseases using imidacloprid 0% and permethrin 50% in young dogs: a longitudinal field study. Vet Parasitol. 72(3-4): Otranto D, Lia RP, Cantacessi C, et al. (2005). Efficacy of a combination of imidacloprid 0%/permethrin 50% versus fipronil 0%/(S)-methoprene 2%, against ticks in naturally infected dogs. Vet Parasitol. 30(3-4): Otranto D, Paradies P, Testini G, et al. (2008). Application of 0% imidacloprid/50% permethrin to prevent Ehrlichia canis exposure in dogs under natural conditions. Vet Parasitol. 53(3-4): Otranto D, Testini G, Dantas-Torres F, et al. (200). Diagnosis of canine vector-borne diseases in young dogs: a longitudinal study. J Clin Microbiol. 48(9): Spencer JA, Butler JM, Stafford KC, et al. (2003). Evaluation of permethrin and imidacloprid for prevention of Borrelia burgdorferi transmission from blacklegged ticks (Ixodes scapularis) to Borrelia burgdorferi-free dogs. Parasitol Res. 90(Suppl 3):S06-S07. Young DR, Arther RG, Davis WL. (2003). Evaluation of K9 Advantix II vs. Frontline Plus topical treatments to repel brown dog ticks (Rhipicephalus sanguineus) on dogs. Parasitol Res. 90(Suppl 3):S6-S8. Mode of action Turberg A, Methfessel C. (2003). Electrophysiological studies demonstrate improved effects with the combination of imidacloprid and permethrin in a pest arthropod. J Vet Pharmacol Ther. 26(Suppl ):9. 26

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31 Contact your Bayer rep today. Order K9 Advantix II Learn about our latest offers and promotions Request promotional material for your practice Inquire about product training REPELLENCY SPEED OF KILL BROAD SPECTRUM CLINIC SUPPORT STUDIES & COMPARISONS

32 Bayer provides solutions for your pest prevention needs. Product Type of client Monthly ticks Topical f leas mosquitoes biting flies lice f leas f lea eggs f lea larvae lice f leas ticks mosquitoes biting flies lice f leas ticks Wants effective protection against fleas, ticks, mosquitoes, biting flies and lice Compliant pet owner Satisfied with monthly flea & tick topical treatments Do not use K9 Advantix II on cats. f leas f lea eggs f lea larvae ticks lice Compliant pet owner f leas ticks Monthly Topical ticksf leas f lea eggs f lea larvae Fleas are main concern Satisfied with monthly flea topical treatments 8-Month Collar f leas ticks Uses fewer than 6 months of treatments per year Prefers a longer duration product than monthly Spray Needs supplemental tick treatment for heavy tick burdens ticks We are continuously monitoring flea susceptibility Each year, thousands of flea eggs are collected by a global network of veterinarians who send samples to investigating laboratories for a three-step analysis. Their research shows that fleas tested continue to remain susceptible to imidacloprid, one of the active ingredients in K9 Advantix II.

33 K9 ADVANTIX II Topical prevention and treatment of ticks, fleas, mosquitoes, biting flies and lice for monthly use only dogs and puppies 7 weeks of age and older READ THE ENTIRE LABEL BEFORE EACH USE DO NOT USE ON CATS For the Prevention and Treatment of Ticks, Fleas, Mosquitoes, Biting Flies and Lice on Dogs Repels and kills ticks including Deer ticks (vector of Lyme disease), American dog ticks (vector of Rocky Mountain spotted fever), Brown dog ticks (vector of Ehrlichiosis), and Lone Star ticks (vector of Ehrlichiosis) for up to four weeks Repels and kills all life stages of fleas Prevents fleas on treated dogs from infesting your home Kills fleas on dogs within 2 hours and continues to prevent infestations for a month Prevents development of fleas, flea eggs, pupae and larvae for a month Larval flea stages in the dog s surroundings are killed following contact with a K9 Advantix II treated dog Repels and kills mosquitoes for up to four weeks Repels and prevents blood-feeding by biting flies Waterproof 5-way protection against ticks, fleas, mosquitoes, biting flies and lice Kills chewing lice ACTIVE INGREDIENTS: % By Weight Imidacloprid % Permethrin* % Pyriproxyfen % OTHER INGREDIENTS % TOTAL % *cis/trans ratio: max 55% (±) cis and min 45% (±) trans KEEP OUT OF REACH OF CHILDREN WARNING DIRECTIONS FOR USE It is a violation of Federal Law to use this product in a manner inconsistent with its labeling. Do not contaminate feed or food. HOW TO OPEN. Be sure tube is at bottom of pouch. 2. Using scissors, cut the pouch across the top and remove tube. HOW TO APPLY. Use only on dogs. DO NOT USE ON CATS or on other animals. Do not apply to dogs weighing less than 4 lbs. 2. Remove applicator tube from the package. Use scissors and peel down foil. 3. Hold applicator tube in an upright position. Pull cap off tube. 4. Turn the cap around and place other end of cap back on tube. 5. Twist cap to break seal, then remove cap from tube. 6. The dog should be standing for easy application. The entire contents of the K9 Advantix II tube should be applied evenly to two to three spots on the top of the back from the shoulder to the base of the tail. At each spot, part the hair until the skin is visible. Place the tip of the tube on the skin and gently squeeze to expel a portion of the solution on the skin. Do not apply an excessive amount of solution at any one spot that could cause some of the solution to run off the side of the dog. Do not get this product in your dog s eyes, or allow your dog to ingest this product. 4-0 lbs.: -20 lbs.: lbs.: Over 55 lbs.: 6 The dog should be standing for easy application. The entire contents of the K9 Advantix II tube should be applied evenly to four to six spots on the top of the back from the shoulder to the base of the tail. At each spot, part the hair until the skin is visible. Place the tip of the tube on the skin and gently squeeze to expel a portion of the solution on the skin. Do not apply an excessive amount of solution at any one spot that could cause some of the solution to run off the side of the dog. Do not get this product in your dog s eyes, or allow your dog to ingest this product. 7. Discard empty tube as described in Storage and Disposal. 8. In cases of severe pest infestations, retreatment is allowable earlier than four (4) weeks. Do not retreat more often than once every seven (7) days. After pest control is attained, return to a monthly retreatment schedule. PRODUCT INFORMATION The successive feeding activity of fleas on dogs may elicit a hypersensitivity skin disorder known as flea allergy dermatitis (FAD). Treatment of dogs with K9 Advantix II rapidly kills fleas and may reduce the incidence of this condition. K9 Advantix II kills existing fleas on dogs within 2 hours. Reinfesting fleas are killed within 2 hours with protection against further flea infestation lasting at least four (4) weeks. Pre-existing pupae in the environment may continue to emerge for six (6) weeks or longer depending upon the climatic conditions. Larval flea stages in the dog s surroundings are killed following contact with a K9 Advantix II treated dog. K9 Advantix II is waterproof and remains effective following a shampoo treatment, swimming or after exposure to rain or sunlight. Monthly treatments are required for optimal control and prevention of fleas and ticks. For dogs over 55 lbs., do not apply more than tube per treatment PRECAUTIONARY STATEMENTS HAZARDS TO HUMANS WARNING. Causes substantial but temporary eye injury. Do not get in eyes or on skin or clothing. Harmful if swallowed. Harmful if absorbed through skin. Avoid contact with skin. Wash thoroughly with soap and water after handling and before eating, drinking, chewing gum, using tobacco or using the toilet. Remove and wash contaminated clothing before reuse. HAZARDS TO DOMESTIC ANIMALS For external use on dogs only. Do not use on animals other than dogs. Do not use on puppies under seven weeks of age or weighing less than 4 lbs. Do not get this product in dog s eyes or mouth. As with any product, consult your veterinarian before using this product on debilitated, aged, pregnant or nursing animals. Individual sensitivities, while rare, may occur after using ANY pesticide product for dogs. If signs persist, or become more severe, consult a veterinarian immediately. If your animal is on medication, consult your veterinarian before using this or any other product. Side Effects Monitor your dog after application, Side effects, although very rare, may include signs of skin irritation such as redness, scratching, or other signs of discomfort. Gastrointestinal signs such as vomiting or diarrhea have also been reported. If these or other side effects (such as lethargy) occur, consult your veterinarian or call DO NOT USE ON CATS MAY BE FATAL. Keep cats away from treated dogs for 24 hours. If applied to a cat or ingested by a cat contact your veterinarian immediately. For consumer questions call For medical emergencies call ENVIRONMENTAL HAZARDS This product is extremely toxic to acquatic organisms, including fish and invertebrates. Do not add directly to water. Do not contaminate water when disposing of product or packaging. FIRST AID If in eyes: Hold eye open and rinse slowly and gently with water for 5 20 minutes. Remove contact lenses, if present, after the first 5 minutes, then continue rinsing eye. Call a poison control center or doctor for treatment advice. If swallowed: Call poison control center or doctor immediately for treatment advice. Have person sip a glass of water if able to swallow. Do not induce vomiting unless told to do so by the poison control center or doctor. Do not give anything by mouth to an unconscious person. If on skin Take off contaminated clothing. or clothing: Rinse skin immediately with plenty of water for 5 20 minutes. Call a poison control center or doctor for treatment advice. HOTLINE NUMBER Have the product container or label with you when calling a poison control center or doctor, or going for treatment. You may also contact for emergency medical treatment information. For consumer questions call NOTE TO PHYSICIAN Treat the patient symptomatically. STORAGE AND DISPOSAL Do not contaminate water, food or feed by storage or disposal. Storage: Store in a cool, dry place. Protect from freezing. Pesticide Disposal and Container Handling: Nonrefillable container. If empty: Do not reuse this container. Place in trash or offer for recycling, if available. If partly filled: Call your local solid waste agency or for disposal instructions. Never place unused product down any indoor or outdoor drain. LIMITED WARRANTY AND LIMITATION OF DAMAGES Bayer HealthCare LLC, Animal Health Division warrants that this material conforms to the chemical description on the label. TO THE EXTENT CONSISTENT WITH APPLICABLE LAW, BAYER MAKES NO OTHER EXPRESS OR IMPLIED WARRANTY, INCLUDING ANY OTHER EXPRESS OR IMPLIED WARRANTY OF FITNESS OR MERCHANTABILITY, and no agent of Bayer is authorized to do so except in writing with a specific reference to this warranty. To the extent consistent with applicable law, any damages arising from a breach of this warranty shall be limited to direct damages and shall not include consequential commercial damages such as loss of profits or values, etc. Manufactured For Bayer HealthCare LLC, Animal Health Division, P.O. Box 390, Shawnee Mission, Kansas 6620 USA Bayer, the Bayer Cross and K9 Advantix are registered trademarks of Bayer 203 Bayer HealthCare LLC Made in Germany K9 Advantix II is protected by the following U.S. patents: 6,232,328 and 7,728,00 K9 ADVANTIX II small dog - dogs and puppies 7 weeks of age and older and weighing 4-0 lbs. Four 0.04 fl oz (0.4 ml) Tubes Six 0.04 fl oz (0.4 ml) Tubes EPA Est. No. 556-DEU- EPA Reg. No K9 ADVANTIX II medium dog - dogs and puppies 7 weeks of age and older and weighing - 20 lbs. Four fl oz (.0 ml) Tubes Six fl oz (.0 ml) Tubes EPA Est. No. 556-DEU- EPA REG. NO K9 ADVANTIX II large dog - dogs and puppies 7 weeks of age and older and weighing 2-55 lbs. Four fl oz (2.5 ml) Tubes Six fl oz (2.5 ml) Tubes EPA Est. No. 556-DEU- EPA Reg. No K9 ADVANTIX II extra large dog - dogs and puppies 7 weeks of age and older and weighing over 55 lbs. Four 0.35 fl oz (4.0 ml) Tubes Six 0.35 fl oz (4.0 ml) Tubes EPA Est. No. 556-DEU- EPA Reg. No

34 No matter how many clients come through your door, only the satisfied ones will continue to put their trust in you time and time again. A perfect fit for client satisfaction There are a lot of options out there, but K9 Advantix II delivers a unique combination of benefits from a repel-and-kill mode of action to ongoing clinic support to help keep your clients satisfied. Weight 20 lbs Weight 2 55 lbs Weight 4 0 lbs Weight Over 55 lbs Do not use K9 Advantix II on cats. Learn more at Bayer HealthCare LLC, Animal Health, Shawnee Mission, Kansas 6620 Bayer (reg d), the Bayer Cross (reg d), Advantage, K9 Advantix, Resultix, Seresto and for the love of dog are trademarks of Bayer. Frontline, NexGard and TriTak are registered trademarks of Merial. Vectra 3D is a registered trademark of Ceva Animal Health, LLC. Activyl and Bravecto are registered trademarks of Intervet Inc., a subsidiary of Merck & Co. Parastar is a registered trademark of Novartis AG. K5008