Population Analysis & Breeding and Transfer Plan

Transcription

1 Population Analysis & Breeding and Transfer Plan African Penguin (Spheniscus demersus) AZA Species Survival Plan Green Program AZA Species Survival Plan Coordinator Steve Sarro, Smithsonian s National Zoo (sarros@si.edu) AZA Species Survival Plan Vice Coordinator Gayle Sirpenski, Mystic Aquarium (gsirpenski@mysticaquarium.org) AZA Studbook Keeper Seana Flossic, Tulsa Zoo (sflossic@tulsazoo.org) AZA Population Advisors Sara Sullivan, Population Management Center, Lincoln Park Zoo (ssullivan@lpzoo.org) 21 September 2016

2 Executive Summary African Penguin (Spheniscus demersus) The African Penguin SSP population at the time of analyses consists of 935 ( ) individuals at 50 AZA institutions. A target size of 1100 individuals was designated for this population by the Penguin Taxon Advisory Group (TAG) in their Regional Collection Plan (RCP). Under AZA s current sustainability designations, this population qualifies as a Green SSP. The genetic statistics reported in this plan are based on an analytical studbook and reflect the exclusion of 167 recently identified potential s that would have otherwise been included as potential breeders. The currently living 714 birds remaining in the potentially breeding population are descended from 94 founders with no additional potential founders remaining. Despite the exclusion of many extra birds in 2016, gene diversity in the population is still very high (98.62%) and equivalent to that found in about 36 unrelated individuals (FGE = 36.18). Furthermore, the temporary loss of these birds as potential breeders has not significantly impacted long term genetic projections, which indicate population gene diversity is at 97% in 100 years and above 95% for over 200 years. Gene diversity of 90% is a standard benchmark for AZA managed populations, and below this level of gene diversity it is expected that reproduction will be increasingly compromised by, among other factors, lower hatch weights, smaller clutch sizes, and greater hatchling mortality. Demography Current size of population (N) Total (Males.Females.Unknown Sex) 935 ( ) Number of individuals excluded from the potentially breeding population 221 ( ) Population size following exclusions 714 ( ) Target population size (Kt) 1100 Mean generation time (years) 9.0 Population Growth Rates 1 (λ; lambda): Historical / 5 year / Projected 1.05 / / Historical from life tables (AZA )/ 5-Year from PopLink Census/ Projected from PMx 20 year stochastic projections Genetics (based on an analytical studbook) Current Potential Founders 94 0 Founder genome equivalents (FGE) Gene diversity (GD %) Population mean kinship (MK) Mean inbreeding (F) % pedigree known before/after exclusions and assumptions 87.9/100 - Effective population size/potentially breeding population (Ne / N) PROJECTIONS 1 λ=1.05; Kt=1100 Years to 90% Gene Diversity > Years to 10% Loss of Gene Diversity > Gene Diversity at 100 Years From Present (%), Assuming Kt = Target sized used in 2016 was = 879 to account for 221 excluded individuals occupying space in the SSP. In order to reach the TAG recommended target size of 1100 in three years (λ=1.053), approximately 126 to 152 hatches are needed. Only approximately 68 hatches are needed to maintain current population size (λ=1.00). With an average of 76.4 hatches recorded in the SSP over the past five years, this population is expected to grow but at a slower rate than is needed to fill SSP spaces by With an additional 77 viable females excluded from breeding this year due to potential ization, SSP institutions will need to increase breeding efforts with the remaining recommended females in order to meet demographic needs. As with most SSP populations, recommended pairings are prioritized to maintain or increase gene diversity through considerations of mean kinship, avoidance of inbreeding, differences in sire and dam mean kinships, and the degree of uncertainty within a pedigree. Every effort was made to keep genetically valuable pairs together and at their current institutions. Additionally, the SSP considered the hypothetical genetic value of potential s and avoided breaking up pairs or moving birds based on their potential status alone. Summary Actions: The SSP recommends 174 breeding females, with 79 of these encouraged to double-clutch. MateRx matrices have been provided for several large colonies to help managers in identifying valuable pairs and regulating reproduction. Approximately 65 transfers have been recommended in order to establish new colonies, create new pairs, and meet institutional needs. Any and all future transactions not listed in these current recommendations must be preapproved by the SSP Steering Committee, including the transfer of offspring produced as a recommendation of the current plan. 1

3 Table of Contents I. Executive Summary 1 II. Description of Population Status 5 Introduction 5 Demography 5 Genetics 7 Management Strategy 8 Explanation of MateRx 10 III. Recommendations Summary recommendations by Studbook ID 11 Recommendations and MateRx by Institution 42 ADVENTURE 42 AQUARAMER 43 BALTIMORE 44 BOS NEAQ 47 BROOKLYN 49 BUSCH TAM, CALDWELL 50 CHICAGOLP, CINCINNAT 51 COLO SPRG, COLUMBUS 52 DALLAS, DALLAS WA 53 DENVER, ERIE 54 FORTWORTH 55 FT WAYNE 56 GA AQUAR 57 GATLINBAQ 59 GREEN NSC 60 GREENBAY 61 JENKINSON, KNOXVILLE 62 LITTLEROC, LOWRY 63 LVZOO, MADISON 64 MEMPHIS 65 MINNESOTA 66 MINOT, MONTERYAQ 68 MYSTIC 69 NEWPORTKY, OMAHA 70 PITTS CA 71 PUEBLO. RACINE 72 ROCHESTER 73 SAGINAW 74 SANDIEGOZ, SPRINGFIE 75 ST PAUL, STEINHART 76 2

4 TAMPA AQ, TAUTPHAUS 77 TOLEDO 78 TORONTO 79 TULSA 80 VANCOUVAQ, W ORANGE 81 IV. Appendices A. Analytical Assumptions 82 B. Summary of Data Exports 85 C. Exclusions 86 D. Life Tables 90 E. Ordered Mean Kinship List 92 F. Descriptive Survival Statistics Report 99 G. Reproductive Report 101 H. Population Viability Analysis (PVA) 2014 Summary Report 104 I. Submission Form for Gender Testing 106 J. Definitions 108 K. Directory of Institutional Representatives 110 3

5 Acknowledgements The master planning session was hosted by The Maryland Zoo in Baltimore on July 2016 and was attended by the following: Steering Committee Attendees: Steve Sarro, SSP Coordinator, Smithsonian National Zoological Park Gayle Sirpenski, SSP Vice-Coordinator, Mystic Aquarium Seana Flossic, Studbook Keeper, Tulsa Zoo & Living Museum Heather Urquhart, New England Aquarium Jennifer Kottyan, The Maryland Zoo in Baltimore Jennifer Odell, Georgia Aquarium Mary Jo Willis, Denver Zoological Gardens Carol Hesch, Memphis Zoological Gardens & Aquarium Chris Gaus, National Aviary in Pittsburgh Ashley Bowen, Pueblo Zoo Megan Klose, Ripley s Aquarium of the Smokies Fred Beall, Zoo New England/ Franklin Park Zoo (Historian) Institutional Representative and Advisor Attendees: Michele Pagel, Adventure Aquarium Darwin Long, Aquarium of the Americas Dr. Ellen Bronson, The Maryland Zoo in Baltimore (Veterinary Advisor) Stephanie Mitchell, New York Aquarium Jessica Hoffman, Natural Science Center of Greensboro Carmen, Murach, NEW Zoo Reagan Quarg, Jenkinson s Aquarium Richard Rosevear, Lehigh Valley Zoo Gary Hartlage, Henry Vilas Zoo (via phone) Kim Fucuda, Monterey Bay Aquarium Elizabeth Wickemeyer, Omaha s Henry Doorly Zoo Vikki McCloskey, Steinhart Aquarium Sara Sullivan, Population Management Center, Lincoln Park Zoo AZA Species Survival Plan Coordinator: Steve Sarro, Smithsonian National Zoological Park, SarroS@si.edu AZA Species Survival Plan Vice Coordinator: Gayle Sirpenski, Mystic Aquarium, gsirpenski@mysticaquarium.org AZA Studbook Keeper: Seana Flossic, Tulsa Zoo, sflossic@tulsazoo.org Analyses and report prepared by: Sara Sullivan, Population Management Center, Lincoln Park Zoo ssullivan@lpzoo.org Cover photo: Steve Sarro This plan was prepared and distributed with the assistance of the Population Management Center in Chicago. pmc@lpzoo.org 4

6 Description of Population Status African Penguin (Spheniscus demersus) SSP Introduction: The African Penguin SSP population at the time of analyses consists of 935 ( ) individuals at 50 AZA institutions. A target size of 1100 individuals was designated for this population by the Penguin Taxon Advisory Group (TAG) in their Regional Collection Plan (RCP). Under AZA s current sustainability designations, this population qualifies as a Green SSP. The African Penguin Species Survival Plan (SSP) was initiated in 1996, and breeding and transfer recommendations have been produced every two years since However, the recommendations presented in this current plan will be reevaluated in a single year s time following molecular investigation into potential ization in the SSP. Comprehensive genetic and demographic analyses of the North American Regional African Penguin Studbook (current to 1 April 2016) were performed in July 2016 using PopLink 2.4 and PMx Version Recommendations contained in this Breeding and Transfer plan supersede those made by earlier plans. Status and Conservation: IUCN (2012): Endangered; CITES: Appendix II; USFWS: Endangered Analytical Population: This population currently has an 87.9% known ancestral pedigree. An analytical overlay was created to address unknowns in the pedigree, based on initial assumptions previously developed by Bob Lacy and additional assumptions created at previous SSP meetings (Appendix A). Fifty-four ( ) birds were excluded from the genetic analyses due to reasons including age, participation in education programs, medical concerns, behavioral issues, and unresolved pedigree (Appendix C). Additionally, 167 ( ) individuals that would have otherwise been included are excluded as potential breeders this year until molecular analysis can confirm species purity. For more information on management of potential s in the SSP, please see page 7. Following these pedigree assumptions and exclusions, the potentially breeding population consists of 714 ( ) individuals with a 100% known pedigree. Demography: The African Penguin SSP population exhibited rapid growth from the early 1970s to the late 1980s due to successful breeding by zoos and aquariums (Figure 1). Growth rates during this time ranged from lambda (λ) of 0.90 to Starting in the 1990s and coinciding with SSP management, annual growth rates due to zoo and aquarium hatches were more modest but with a general trend of positive growth (mean λ over past 26 years = 1.033). In the last five years, the population has grown on average by about 3% (λ = 1.034). In order to reach the TAG recommended target size of 1100 in three years (λ=1.053), approximately 126 to 152 hatches are needed. Only approximately 68 hatches are needed to maintain current population size (λ=1.00). With an average of 76.4 hatches recorded in the SSP over the past five years, this population is expected to grow but at a slower rate than is needed to fill SSP spaces by With an additional 77 viable females excluded from breeding this year due to potential ization, SSP institutions will need to increase breeding efforts with the remaining breeding females in order to meet demographic needs. Figure 1. Census of the African Penguin SSP population from 1960 to present by sex (left) and origin (right). 5

7 Figure 2. Age distribution of the total African Penguin SSP population before exclusions (left; N = 935 ( )) and the potentially breeding population after 221 exclusions (right; N = 714 ( )). Note the scale difference on the x axis. Age distribution The age structure of the total African Penguin SSP population appears fairly stable, with no empty age classes and a large base of juveniles to support future reproduction and demographic stability (Fig. 2, left). Even after the exclusion of 221 birds, 23% of the total population, the potentially breeding population still approximates a stable distribution with many breeding males and females (Fig. 2, right). In both cases, a male sex bias is evident, which could potentially disrupt breeding in this monogamously breeding species; however, unpaired males can be managed in single sexed colonies when necessary. Due to the large size of this population and the specie s long reproductive lifespan, this is not a major concern currently but should continue to be monitored in the future. Longevity According to studbook data in AZA between 1970 to present, if an African penguin in AZA survives past the first year of life, median life expectancy is 18.6 years for males and 15.3 years for females (Appendix F). This indicates that about half of animals hatched in AZA that survive past the first year of life are expected to die before these ages and about half after. Although the studbook also contains records of African Penguins living upwards of 41 years old for both sexes, median life expectancy is a more appropriate way to communicate the typical lifespan of this species in AZA zoos. The studbook also contains a record for a wild hatched male living to 45 years (with a +/- year estimate); however, because hatch dates and ages at entry into zoos are unknown and difficult to verify, 41 years should be viewed as a more reliable maximum longevity until more data accumulate. First year mortality observed for this species is approximately 32% for males and 31% for females (Appendix D). As with most egg laying species, only eggs which hatch and are recorded in the studbook are reflected in these mortality rates. Reproduction Reproduction has been recorded as early as 1.5 years for both sexes, though first reproduction at around two years is more typical. Peak female reproductive range, based on studbook data in AZA from 1970 to present, occurs between four and 15 years, though a decline in fecundity is noticed after eight years. Nonetheless, females have reproduced at as old as 32 years. Males have a longer reproductive span, with the oldest sire recorded at 35 years and fairly consistent fecundity rates observed between four and 28 years. However, decline in fecundity is evident after age class 14. Reproduction has been recorded year round. Clutch size ranges from one to two (mean = 1.4) following an approximately 38 day incubation period. Multiple clutches in a single season are possible and will be needed to meet demographic needs this year. 6

8 Genetics: These genetic statistics are based on an analytical studbook with pedigree assumptions intended to estimate relatedness. They also reflect the exclusion in 2016 of 167 recently identified potential s that would have otherwise been included as potential breeders. These animals will remain excluded until species purity can be verified through molecular genetic analysis. Consequently, population statistics reported in this plan may not be comparable to previous years and could change again in the future. The currently living 714 birds remaining in the potentially breeding population are descended from 94 founders with no additional potential founders remaining. Despite the exclusion of many extra birds in 2016, gene diversity in the population is still very high (98.62%) and equivalent to that found in about 36 unrelated individuals (FGE = 36.18). Furthermore, the temporary loss of these birds as potential breeders has not significantly impacted long term genetic projections, which indicate population gene diversity is at 97% in 100 years and above 95% for over 200 years. Gene diversity of 90% is a standard benchmark for AZA managed Figure 3. Founder representation graph illustrating the inequality of founder lineages in the potentially breeding SSP population. populations, and below this level of gene diversity it is expected that reproduction will be increasingly compromised by, among other factors, lower hatch weights, smaller clutch sizes, and greater hatchling mortality. Strategies to maintain gene diversity include equalization of founder representation (Fig. 3) by breeding animals with low and well matched mean kinships and through maintenance of the already high effective size ratio (proportion of population breeding). At this time, the importation of new founders is not necessary to meet genetic goals. Genetic Summary Potential Founders Founder genome equivalents (FGE) Gene diversity (GD %) Population mean kinship (MK) Mean inbreeding (F) Percentage of pedigree known before exclusions and assumptions Percentage of pedigree known after exclusions and assumptions Effective population size ratio (Ne /N) PROJECTIONS 2, λ=1.05, Kt= Years To 90% Gene Diversity >200 > 200 > 200 >200 >200 - Years to 10% Loss of Gene Diversity -- > 200 > 200 >200 >200 - Gene Diversity at 100 Years From Present Genetic statistics are based on an analytical studbook with pedigree assumptions that may over or under-estimate relatedness. 2 Projections may not be comparable across years due to differences in assumptions, exclusions, target sizes, λ, etc. 3 Target sized used in 2016 was = 879 to account for 221 excluded individuals occupying space in the SSP. 7

9 Management of ization: Molecular genetic testing recently confirmed an African x Humboldt penguin in the European Endangered Species Programme (EEP). The original ization event is suspected to have occurred before 1982 in STUTTGART, which held African and Humboldt penguins in the same enclosure until Consequently, any individual with STUTTGART ancestry is considered to have a high ization risk. In order to identify other potential ization events, the EEP is investigating historic mixed colonies of African and Humboldt Penguins through comparative analysis of their respective EEP studbooks (Bos, Schad, & Fienieg, 2016). Through this comparative analysis, the EEP identified 20 individuals exported to the SSP that have ancestry tracing back to mixed exhibits in the EEP. The SSP imported these individuals during two events, the first in 1984 from AMSTERDAM to BALTIMORE and the second in 2006 from LISBON to GA AQUARIUM. Of these 20 imports, 14 have currently living descendants in the SSP. Ten of the living descendants are permanent non-breeders, which means that 167 birds that would otherwise have been included as potential breeders are excluded this year. These constitutes approximately 18% of the total SSP population (167/935; Fig. 4). However, due to its large size, the SSP population can afford to exclude reproductively viable potential s as breeders in the short term until molecular analyses can confirm species purity. It should be noted that the fact that these birds trace back to mixed Figure 4. This graph illustrates the proportion of the total SSP population (N=935) that is (a) excluded as permanent non-breeders, (b) reproductively viable but temporarily excluded as breeders due to potential status (18%), and (c) currently included as potential breeders (76%). African and Humboldt penguin exhibits in Europe only indicates potential of ization not confirmation. The SSP is currently working to identify the most feasible and cost effective way to collect blood samples for analysis. Any potential confirmed to be purebred will be added back into the potentially breeding population in the future. In these current recommendations, the SSP considered the hypothetical genetic value of potential s and avoided breaking up pairs or moving birds based on their potential status alone. Management Strategy: In order to reach the TAG recommended target size of 1100 in three years (λ=1.053), approximately 126 to 152 hatches are needed. Only approximately 68 hatches are needed to maintain current population size (λ=1.00). With an average of 76.4 hatches recorded in the SSP over the past five years, this population is expected to grow but at a slower rate than is needed to fill SSP spaces by With an additional 77 viable females excluded from breeding this year due to potential ization, SSP institutions will need to increase breeding efforts with the remaining recommended females in order to meet demographic needs. As with most SSP populations, recommended pairings are prioritized to maintain or increase gene diversity through considerations of mean kinship, avoidance of inbreeding, differences in sire and dam mean kinships, and the degree of uncertainty within a pedigree. Every effort was made to keep genetically valuable pairs together and at their current institutions. Additionally, the SSP considered the hypothetical genetic value of potential s and avoided breaking up pairs or moving birds based on their potential status alone. In order to meet demographic goals, some demographic pairs, consisting of individuals with higher than average mean kinships and/or low levels of relatedness, have been recommended. The SSP made every attempt to avoid breeding between genetically mismatched individuals, which would only flood the population with common alleles, permanently link rare and common lineages, and make pairing recommendations increasingly difficult in the future. The studbook keeper will request an update from all breeding facilities in approximately one year, and the management group will closely monitor the breeding success and make adjustments if necessary. Revised recommendations may be distributed in 2017 if the SSP is able to resolve questionable species purity by that time. 8

10 1. The SSP recommends 174 breeding females among 37 institutions. a. These breeding recommendations apply to the upcoming year and may be revised again by the end of 2017 if questions concerning the potential issue have been resolved by this time. For more information on how the SSP is managing potential s in the population, please refer to page 8. b. Approximately 79 recommended breeding pairs may double clutch in the upcoming year. c. Six institutions with large colonies have been provided with MateRx matrices in order to help them to work with the SSP Coordinator in identifying genetically valuable pairs and regulating reproduction. For more information on using MateRx, see page 10. Any breeding pairs with MSI scores of 1, 2, or 3 are recommended to double clutch. However, contact the SSP Coordinators first for approval to breed any potential pairs using the MateRx matrix. 2. The SSP recommends 65 transfers in order to create new breeding pairs and to meet institutional needs. An additional five animals are available currently for placement. 3. Attempts were made to address existing pairs with specific recommendations. The SSP considered the hypothetical genetic value of potential s and avoided breaking up pairs or moving birds based on their potential status alone. Institutions should consult with the SSP Coordinators with questions about pairs or re-pairings not addressed in this plan. 4. Recommendations for breeding birds must await determination of sex. Jean Dubach at Loyola Medical Center is willing to use DNA probes to determine the sex of any African penguin, at a low cost to the SSP zoos. A submission form with detailed instructions for this test is attached (Appendix I). 5. The SSP wants to remind all institutions to check the ownership of any birds that are recommended to transfer. It is the responsibility of the holding institution to inform the owner about the transfer. 6. The SSP considers all individuals, of all participating institutions, to be a part of the SSP population. Therefore, unless recommended by the current Breeding and Transfer Plan, any and all transactions must be pre-approved by the SSP Steering Committee, including the transfer of offspring produced as a recommendation of the current plan. Individuals that are not recommended for breeding should not be bred, and no offspring are to be produced outside of these breeding recommendations. Consult the SSP Coordinators if you have any questions on this matter. 7. Institutions interested in creating a new penguin enclosure or expanding their current one are encouraged to explore the possibility of holding Humboldt penguins rather than African penguins. If you are interested, contact the Humboldt penguin SSP Coordinator, Alex Waier, at Alexander.Waier@milwaukeecountywi.gov. 9

11 Explanation of Recommendations Using MateRx MateR x is analytical software developed jointly by the National Zoological Park and Lincoln Park Zoo. The primary output is a matrix of genetic ratings (Mate Suitability Indices = MSI) for every possible breeding pair in a population. MSIs allow managers to quickly discover how the genetic status of animals in their collections and compare to the rest of a managed population. Each MSI represents the genetic consequences for the population if a given pair was to produce offspring. There are seven values for MSIs: offspring of pairs rated 1, 2, or 3 would benefit the population s genetic situation; pairs rated 4, 5, or 6 would be detrimental to the population s genetic situation. Pairs without an MSI value (i.e., a dash [--]) should not be considered under any circumstances without consulting a PMC, PMC Adjunct, or SPMAG advisor. These MSI values are defined as: 1 very beneficial genetically (recommended to double clutch) 2 moderately beneficial (recommended to double clutch) 3 slightly beneficial (recommended to double clutch) 4 neutral 5 moderately detrimental 6 very detrimental genetically MateR x integrates four genetic factors to produce the Mate Suitability Index (MSI). These four components are used by PMC, PMC Adjunct, or SPMAG advisors to develop breeding recommendations. In decreasing order of importance, the components used to rate pairings are: 1. the expected change in genetic diversity (increase, decrease) that would result if an offspring of a pair is added to the population; 2. the relative rareness or commonness of the parents genetic information (i.e., the difference between the male and female mean kinships); 3. the inbreeding coefficient of offspring that would be produced by a pair; and 4. the proportion, if any, of the dam and/or sire s pedigree that is of unknown origin. 10

12 Summary of Breeding and Transfer Recommendations 2016 by Studbook Number ( animals =, Available animals = ) ID Location Local ID Sex Age Disposition Location Breeding With Notes 298 OMAHA 3779 F 39 HOLD OMAHA 429 ST PAUL 3586 F 35 HOLD ST PAUL 461 ST PAUL 1282 M 35 HOLD ST PAUL 487 PUEBLO M 35 HOLD PUEBLO 500 BOS NEAQ F 34 HOLD BOS NEAQ 519 MADISON 1277 M 34 HOLD MADISON 526 AQUARAMER S3 M 34 HOLD AQUARAMER 527 MADISON 1276 F 34 HOLD MADISON 588 TULSA M 33 HOLD TULSA 604 MADISON 1315 F 33 HOLD MADISON 624 MADISON 1320 M 33 HOLD MADISON 649 MINOT A35 M 33 HOLD MINOT 660 DENVER M 32 HOLD DENVER 683 CINCINNAT M 31 HOLD CINCINNAT 715 STEINHART F 32 HOLD STEINHART 716 BOS NEAQ 8466 F 32 HOLD BOS NEAQ 727 FORTWORTH F 32 HOLD FORTWORTH 747 RACINE 9231 F 31 HOLD RACINE 755 CINCINNAT F 31 HOLD CINCINNAT 816 BALTIMORE M 30 HOLD BALTIMORE 833 MADISON 1508 F 30 HOLD MADISON 839 JENKINSON 198 F 30 HOLD JENKINSON 840 GA AQUAR B09017 M 30 HOLD GA AQUAR 842 JENKINSON 199 F 30 HOLD JENKINSON 852 MADISON 1471 M 30 HOLD MADISON 872 MADISON 1516 F 29 HOLD MADISON 878 BALTIMORE F 29 HOLD BALTIMORE 937 GA AQUAR B09018 F 29 HOLD GA AQUAR NOTES Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age Age; Keep any fertile eggs produced by 1378 x 842 Age/ Unknown pedigree Age Age Age 11

13 ID Location Local ID Sex Age Disposition Location Breeding With Notes 952 CINCINNAT F 29 HOLD CINCINNAT 954 MYSTIC 3 M 29 HOLD MYSTIC 959 TAUTPHAUS K0A029 M 29 HOLD TAUTPHAUS 965 MYSTIC 7 M 28 HOLD MYSTIC 973 ADVENTURE 8 M 28 HOLD ADVENTURE 974 MYSTIC 8 F 28 HOLD MYSTIC 983 MEMPHIS M 28 HOLD MEMPHIS 987 MYSTIC 11 F 28 HOLD MYSTIC 990 MYSTIC 9 M 28 HOLD MYSTIC 998 CINCINNAT M 28 HOLD CINCINNAT 1016 KNOXVILLE 367 F 28 HOLD KNOXVILLE 1019 MYSTIC 4 F 28 HOLD MYSTIC 1028 STEINHART F 28 HOLD STEINHART 1111 AQUARAMER R3 M 27 HOLD AQUARAMER 1147 GA AQUAR B10002 F 27 HOLD GA AQUAR 1166 TULSA F 27 HOLD TULSA 1184 KNOXVILLE 369 F 27 HOLD KNOXVILLE 1192 BOS NEAQ M 26 HOLD BOS NEAQ 1203 OMAHA 5666 F 26 HOLD OMAHA 1204 DALLAS 06G547 M 26 HOLD DALLAS 1213 OMAHA 5668 M 26 HOLD OMAHA 1220 AQUARAMER R7 M 26 HOLD AQUARAMER 1240 AQUARAMER S6 F 26 HOLD AQUARAMER 1263 BOS NEAQ M 26 HOLD BOS NEAQ 1270 TORONTO M 26 HOLD TORONTO 1278 FORTWORTH M 26 HOLD FORTWORTH 1299 AQUARAMER S5 F 26 HOLD AQUARAMER 1378 JENKINSON 200 M 28 HOLD JENKINSON 1384 MYSTIC SD9101 M 25 HOLD MYSTIC 1392 PUEBLO F 25 HOLD PUEBLO 1393 PUEBLO F 25 HOLD PUEBLO 1396 SANDIEGOZ M 25 HOLD SANDIEGOZ NOTES Age Age Age Age/ Age Age/ Medical Age Age/ Age Age Age Age/ Medical Paired with 842; keep fertile eggs from this pair Age 12

14 ID Location Local ID Sex Age Disposition Location Breeding With Notes 1397 JENKINSON 874 M 25 HOLD JENKINSON 1408 ST PAUL 1675 M 25 HOLD ST PAUL 1409 TULSA F 25 HOLD TULSA 1413 RACINE M0716 F 25 HOLD RACINE 1445 KNOXVILLE 260 F 25 HOLD KNOXVILLE 1448 AQUARAMER A1 F 25 HOLD AQUARAMER 1457 W ORANGE 4852 M 24 HOLD W ORANGE 1462 W ORANGE 4854 M 24 HOLD W ORANGE 1466 MINOT A132 M 24 HOLD MINOT 1473 LITTLEROC 6984 F 24 HOLD LITTLEROC 1477 DENVER A06140 F 24 HOLD DENVER 1479 ADVENTURE 9 F 24 HOLD ADVENTURE 1480 SANDIEGOZ F 24 HOLD SANDIEGOZ 1482 BROOKLYN M 24 HOLD BROOKLYN 1484 STEINHART M 24 HOLD STEINHART 1485 MINOT A44 M 24 HOLD MINOT 1492 ST PAUL 1699 F 24 HOLD ST PAUL 1494 BROOKLYN M 24 HOLD BROOKLYN 1496 STEINHART F 24 HOLD STEINHART 1501 TORONTO M 24 HOLD TORONTO 1503 FORTWORTH F 24 HOLD FORTWORTH 1509 JENKINSON 875 M 24 HOLD JENKINSON 1520 BALTIMORE M 26 HOLD BALTIMORE 1521 BOS NEAQ F 25 HOLD BOS NEAQ 1525 BALTIMORE F 25 HOLD BALTIMORE 1528 BALTIMORE F 25 HOLD BALTIMORE Age/ Medical/ Imprinted Age/Education Valuable pair - double clutch; lower likelihood of success (have not been producing fertile eggs) Demographic pair but low likelihood of success expected due to age Double clutch, though low likelihood of success expected due to age Double clutch, though low likelihood of success expected due to age 13

15 ID Location Local ID Sex Age Disposition Location Breeding With Notes 1543 OMAHA 6954 M 24 HOLD OMAHA 1544 MONTERYAQ M 24 HOLD MONTERYAQ 1545 GA AQUAR B10001 M 24 HOLD GA AQUAR 1552 TAUTPHAUS 16A013 M 24 HOLD TAUTPHAUS 1571 BOS NEAQ M 24 HOLD BOS NEAQ 1576 LOWRY M 24 HOLD LOWRY 1586 BALTIMORE M 24 HOLD BALTIMORE 1610 MONTERYAQ ABFP12 F 23 HOLD MONTERYAQ 1629 GATLINBAQ GB-SD M M 23 HOLD GATLINBAQ 1630 BROOKLYN F 23 HOLD BROOKLYN 1643 DALLAS 06G548 F 23 SEND TO MEMPHIS 1645 BOS NEAQ F 23 HOLD BOS NEAQ 1650 TULSA F 23 HOLD TULSA 1654 MINNESOTA F 23 HOLD MINNESOTA 1661 KNOXVILLE 520 M 23 HOLD KNOXVILLE 1663 OMAHA 6966 F 23 HOLD OMAHA 1669 TULSA F 23 HOLD TULSA 1672 BALTIMORE UNK M 23 HOLD BALTIMORE 1677 MYSTIC SD9208 F 23 HOLD MYSTIC 1688 BOS NEAQ M 23 HOLD BOS NEAQ 1689 JENKINSON 997 M 23 HOLD JENKINSON 1698 OMAHA 7145 M 23 HOLD OMAHA 1703 MYSTIC SD9302 F 23 HOLD MYSTIC 1707 ERIE 1308 F 23 HOLD ERIE 1721 MINNESOTA F 23 HOLD MINNESOTA 1732 COLO SPRG 23A006 M 23 SEND TO BROOKLYN 1735 MINNESOTA M 23 HOLD MINNESOTA 1739 ST PAUL 1784 M 22 HOLD ST PAUL 1753 MINNESOTA F 22 HOLD MINNESOTA 1761 BOS NEAQ F 22 HOLD BOS NEAQ 1766 COLUMBUS M 22 HOLD COLUMBUS Age/ Medical/ Potential 2388 Double clutch 2946 Double clutch 2885 Demographic pair Genetically valuable older female - priority to breed 1735 Double clutch

16 ID Location Local ID Sex Age Disposition Location Breeding With Notes 1768 COLUMBUS F 22 HOLD COLUMBUS 1770 TAUTPHAUS 97A016 M 22 HOLD TAUTPHAUS 1784 PUEBLO B32011 M 22 SEND TO DALLAS 1787 CALDWELL M 22 HOLD CALDWELL 1796 FORTWORTH F 23 HOLD FORTWORTH 1803 OMAHA 7646 M 22 HOLD OMAHA 1807 COLO SPRG 23A007 F 22 SEND TO BROOKLYN 1809 BOS NEAQ M 22 HOLD BOS NEAQ 1811 FORTWORTH F 22 HOLD FORTWORTH 1815 FORTWORTH M 22 HOLD FORTWORTH 1820 GA AQUAR B09011 F 22 HOLD GA AQUAR 1823 MYSTIC SD9402 M 22 HOLD MYSTIC 1829 BOS NEAQ M 22 HOLD BOS NEAQ 1841 TOLEDO 5512 M 22 HOLD TOLEDO 1842 TAUTPHAUS 97A012 M 22 HOLD TAUTPHAUS 1844 MONTERYAQ M 22 HOLD MONTERYAQ 1848 MONTERYAQ F 22 HOLD MONTERYAQ 1854 GREENBAY F 22 HOLD GREENBAY 1868 GREENBAY M 21 HOLD GREENBAY 1869 ROCHESTER F 21 HOLD ROCHESTER 1870 TAUTPHAUS 97A001 F 21 HOLD TAUTPHAUS 1883 MONTERYAQ F 21 HOLD MONTERYAQ 1886 TAUTPHAUS 97A002 F 21 HOLD TAUTPHAUS 1890 GREENBAY F 21 HOLD GREENBAY 1895 TAUTPHAUS 97A004 F 21 HOLD TAUTPHAUS 1902 MINNESOTA M 21 HOLD MINNESOTA 1903 RACINE M1525 M 21 HOLD RACINE 1904 JENKINSON 1672 F 21 HOLD JENKINSON 1913 BROOKLYN A10002 F 21 HOLD BROOKLYN NOTES Double clutch 2157 Double clutch 1796 Valuable older female - priority for breeding. If she pairs with 2337, 2396, or 3144, keep fertile eggs 2198 Double clutch Double clutch but low likelihood of success expected due to age Demographic pair - lower likelihood of success expected due to male's health 1770 Double clutch 15

17 ID Location Local ID Sex Age Disposition Location Breeding With Notes 1929 JENKINSON 1747 F 21 HOLD JENKINSON 1931 JENKINSON 1788 M 21 HOLD JENKINSON 1933 BOS NEAQ M 21 HOLD BOS NEAQ 1939 PUEBLO M 20 HOLD PUEBLO 1943 BOS NEAQ F 20 HOLD BOS NEAQ 1948 ADVENTURE 4079 F 20 HOLD ADVENTURE 1960 TOLEDO 2189 M 20 HOLD TOLEDO 1962 ROCHESTER M 20 HOLD ROCHESTER 1963 JENKINSON 1930 F 20 HOLD JENKINSON 1976 ROCHESTER M 20 HOLD ROCHESTER 1977 GREENBAY M 20 HOLD GREENBAY 1979 ADVENTURE 5 F 20 HOLD ADVENTURE 1984 TORONTO F 20 HOLD TORONTO 2002 TOLEDO 5513 M 20 HOLD TOLEDO 2003 OMAHA 9045 F 20 HOLD OMAHA 2022 PUEBLO B24001 F 20 HOLD PUEBLO 2027 BALTIMORE F 19 HOLD BALTIMORE 2036 BALTIMORE M 19 HOLD BALTIMORE 2037 ERIE 1674 M 19 HOLD ERIE 2041 MYSTIC SD33 F 19 HOLD MYSTIC 2045 GATLINBAQ GB-SD F F 19 HOLD GATLINBAQ 2047 MYSTIC SD9701 M 19 HOLD MYSTIC 2050 VANCOUVAQ F 19 HOLD VANCOUVAQ 2051 BOS NEAQ M 19 HOLD BOS NEAQ 2052 BROOKLYN A11210 F 18 HOLD BROOKLYN 1397 Double clutch 2058 KNOXVILLE 3030 M 18 SEND TO ST PAUL 2061 ROCHESTER M 23 HOLD ROCHESTER ROCHESTER M 21 HOLD ROCHESTER 2063 ROCHESTER M 21 HOLD ROCHESTER 2065 ROCHESTER F 21 HOLD ROCHESTER 2203 Double clutch 2066 BOS NEAQ M 21 HOLD BOS NEAQ ADVENTURE 18 M 18 HOLD ADVENTURE Demographic pair 1854 Medical Can breed with 3055 as an alternative 2077 Demographic pair 2135 Double clutch Demographic pair; Repair

18 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2081 TULSA M 18 HOLD TULSA 2083 BALTIMORE F 18 HOLD BALTIMORE 2084 W ORANGE 6109 F 18 HOLD W ORANGE 2085 ERIE 1304 M 18 HOLD ERIE 2088 LVZOO LV0239 M 19 HOLD LVZOO 2089 LVZOO LV0240 M 19 HOLD LVZOO 2090 TORONTO M 19 HOLD TORONTO 2101 GATLINBAQ GB-SD M M 18 HOLD GATLINBAQ 2103 TULSA M 18 HOLD TULSA 2104 BALTIMORE F 18 HOLD BALTIMORE 2106 BALTIMORE M 18 HOLD BALTIMORE 2107 MYSTIC SD9801 M 18 HOLD MYSTIC 2108 MYSTIC SD9802 M 18 HOLD MYSTIC 2112 OMAHA 9625 F 19 HOLD OMAHA 2116 FT WAYNE M 19 HOLD FT WAYNE 2118 DALLAS 06G552 M 18 HOLD DALLAS 2119 ADVENTURE 20 M 18 HOLD ADVENTURE 2123 BROOKLYN M 18 HOLD BROOKLYN 2126 OMAHA M 18 HOLD OMAHA 2130 TULSA F 18 HOLD TULSA 2131 COLO SPRG 23A009 M 18 SEND TO BROOKLYN 2135 MINOT A299 M 17 SEND TO PUEBLO 2136 W ORANGE 6110 F 17 HOLD W ORANGE 2138 TULSA M 17 HOLD TULSA 2139 BOS NEAQ F 18 HOLD BOS NEAQ 2140 BOS NEAQ F 18 HOLD BOS NEAQ 2141 BOS NEAQ M 17 HOLD BOS NEAQ 1672 Demographic pair 2142 BOS NEAQ M 17 HOLD BOS NEAQ 2143 BOS NEAQ F 17 HOLD BOS NEAQ 2144 COLO SPRG 23A010 M 17 SEND TO BROOKLYN 2145 CALDWELL F 17 HOLD CALDWELL 2367 Demographic pair Demographic pair Valuable but some relatedness - one clutch per year Double clutch; Female 2419 ok as an alternative

19 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2146 CINCINNAT M 17 HOLD CINCINNAT 2147 BALTIMORE M 17 HOLD BALTIMORE 2157 COLO SPRG 23A011 M 17 SEND TO BROOKLYN 2158 TAUTPHAUS 12A016 F 17 HOLD TAUTPHAUS 2167 FT WAYNE F 17 HOLD FT WAYNE 2168 ST PAUL 3585 M 17 HOLD ST PAUL 2171 FT WAYNE M 18 HOLD FT WAYNE 2172 FT WAYNE F 18 HOLD FT WAYNE 2173 OMAHA F 18 HOLD OMAHA 2176 CINCINNAT M 18 HOLD CINCINNAT 2179 BALTIMORE UNK M 17 HOLD BALTIMORE 2187 CINCINNAT M 17 HOLD CINCINNAT 2188 MONTERYAQ M 17 HOLD MONTERYAQ 2192 OMAHA M 17 HOLD OMAHA 2194 MEMPHIS F 17 HOLD MEMPHIS 2195 TULSA M 17 HOLD TULSA 2196 MONTERYAQ M 17 HOLD MONTERYAQ 2198 MONTERYAQ M 17 HOLD MONTERYAQ 2203 ROCHESTER M 17 HOLD ROCHESTER 2208 MONTERYAQ M 17 HOLD MONTERYAQ 2210 BALTIMORE 99B022 M 17 HOLD BALTIMORE 2222 MINOT A300 M 17 HOLD MINOT 2226 MONTERYAQ F 17 HOLD MONTERYAQ 2230 BALTIMORE 99B038 M 17 HOLD BALTIMORE 2234 FORTWORTH F 17 HOLD FORTWORTH 2238 JENKINSON 2629 F 17 HOLD JENKINSON 2271 OMAHA M 17 HOLD OMAHA 2279 MEMPHIS M 16 HOLD MEMPHIS 2290 ROCHESTER M 16 HOLD ROCHESTER 2291 MYSTIC SD36 M 16 HOLD MYSTIC 2293 TOLEDO 3280 M 16 HOLD TOLEDO Double clutch Unknown pedigree Genetically valuable Unknown pedigree 18

20 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2294 TULSA M 16 HOLD TULSA 2295 DALLAS WA 14A034 M 16 HOLD DALLAS WA 2296 COLO SPRG 23A015 M 16 SEND TO BROOKLYN 2310 ROCHESTER F 16 HOLD ROCHESTER 2315 TOLEDO 3578 M 15 HOLD TOLEDO 2316 TULSA M 15 HOLD TULSA 2317 COLO SPRG 23A016 F 15 SEND TO BROOKLYN 2319 MEMPHIS F 15 HOLD MEMPHIS 2320 TULSA M 15 HOLD TULSA 2321 ADVENTURE 3252 F 15 HOLD ADVENTURE 2322 BALTIMORE 821 F 15 HOLD BALTIMORE 2323 TOLEDO 3579 M 15 HOLD TOLEDO 2329 BALTIMORE 905 F 15 HOLD BALTIMORE 2331 FORTWORTH M 15 HOLD FORTWORTH 2333 BALTIMORE 917 M 15 HOLD BALTIMORE 2336 ROCHESTER F 15 HOLD ROCHESTER 2337 FORTWORTH M 15 HOLD FORTWORTH 2340 TOLEDO 9490 M 15 HOLD TOLEDO 2347 CINCINNAT M 15 HOLD CINCINNAT 2348 MEMPHIS M 15 HOLD MEMPHIS 2353 TORONTO M 15 HOLD TORONTO 2356 OMAHA F 15 HOLD OMAHA 2357 AQUARAMER FW1 M 15 HOLD AQUARAMER 2358 TULSA F 15 HOLD TULSA 2360 BOS NEAQ M 15 HOLD BOS NEAQ 2361 BALTIMORE 1359 M 14 HOLD BALTIMORE 2367 CALDWELL M 14 HOLD CALDWELL 2370 BALTIMORE 1880 F 14 HOLD BALTIMORE 2371 SPRINGFIE 1013 F 14 HOLD SPRINGFIE 2408 Demographic pair Paired with related male 2333 Paired with related female 2329 If he pairs with valuable older female 1811, keep fertile eggs Demographic pair; Male 2147 is ok as an alternative 19

21 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2372 LITTLEROC 7047 M 14 HOLD LITTLEROC 2373 BALTIMORE UNK F 15 HOLD BALTIMORE 2374 BOS NEAQ F 14 HOLD BOS NEAQ 2375 STEINHART M 14 HOLD STEINHART 2379 MONTERYAQ F 14 HOLD MONTERYAQ 2380 FT WAYNE M 14 HOLD FT WAYNE 2381 RACINE M0187 M 14 SEND TO GREENBAY 2383 CALDWELL M 13 HOLD CALDWELL 2384 MYSTIC SD0201 F 14 HOLD MYSTIC 2387 BALTIMORE 5999 F 14 SEND TO LVZOO 2388 MEMPHIS M 14 HOLD MEMPHIS 2394 W ORANGE 6276 M 13 HOLD W ORANGE 2396 FORTWORTH M 13 HOLD FORTWORTH 2397 FORTWORTH F 13 HOLD FORTWORTH 2399 FORTWORTH F 13 HOLD FORTWORTH 2402 CALDWELL M 13 HOLD CALDWELL 2403 CALDWELL F 13 HOLD CALDWELL 2405 PITTS CA 6157 M 14 HOLD PITTS CA 2407 ADVENTURE 87 M 14 HOLD ADVENTURE 2408 ADVENTURE 119 M 14 HOLD ADVENTURE 2409 OMAHA M 14 HOLD OMAHA 2410 LOWRY F 16 HOLD LOWRY 2413 ADVENTURE 132 F 13 HOLD ADVENTURE 2414 OMAHA M 13 HOLD OMAHA 2415 RACINE M0306 M 13 SEND TO GREENBAY 2417 CALDWELL F 13 HOLD CALDWELL 2418 TOLEDO 9702 M 14 HOLD TOLEDO 2419 BOS NEAQ F 14 HOLD BOS NEAQ 2420 ERIE 1326 M 14 HOLD ERIE 2427 TULSA F 13 HOLD TULSA 2433 ROCHESTER F 12 HOLD ROCHESTER NOTES Demographic pair Double clutch 1643 If he pairs with valuable older female 1811, keep fertile eggs 2337 Double clutch 2817 Demographic pair Double clutch Demographic pair 20

22 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2434 BALTIMORE 4375 F 12 HOLD BALTIMORE 2445 CALDWELL M 12 HOLD CALDWELL 2446 PITTS CA 6942 F 12 HOLD PITTS CA 2447 ST PAUL 4017 F 12 HOLD ST PAUL 2448 ST PAUL 4018 M 12 HOLD ST PAUL 2451 DENVER A05444 F 12 HOLD DENVER 2454 ERIE 1481 F 12 HOLD ERIE 2456 PITTS CA 7232 M 11 HOLD PITTS CA 2460 ERIE 1482 F 11 HOLD ERIE 2463 BALTIMORE 7435 M 12 HOLD BALTIMORE 2464 BOS NEAQ F 12 HOLD BOS NEAQ 2469 BALTIMORE 4880 F 11 HOLD BALTIMORE 2470 OMAHA M 11 HOLD OMAHA 2472 BALTIMORE 4885 F 11 HOLD BALTIMORE 2473 BALTIMORE 4887 M 11 HOLD BALTIMORE 2474 PUEBLO B25023 M 12 SEND TO CHICAGOLP 2475 KNOXVILLE 3657 F 12 SEND TO ST PAUL 2476 DENVER A04169 M 12 HOLD DENVER 2478 DALLAS WA 14A032 M 11 HOLD DALLAS WA 2480 MADISON 2708 F 11 HOLD MADISON 2483 DENVER A05445 F 11 HOLD DENVER 2485 BALTIMORE 5492 M 11 HOLD BALTIMORE 2496 SAGINAW F 14 HOLD SAGINAW 2497 LVZOO LV0431 F 14 HOLD LVZOO 2498 SAGINAW M 14 HOLD SAGINAW 2499 SAGINAW F 14 HOLD SAGINAW 2504 SAGINAW F 13 SEND TO BUSCH TAM 2508 SAGINAW F 13 HOLD SAGINAW 2525 TULSA F 13 HOLD TULSA 2526 PITTS CA 6658 M 12 HOLD PITTS CA 2529 GREENBAY M 12 SEND TO RACINE WTH 2746 Demographic pair Double clutch; Repair 1571 Double clutch 2473 Double clutch 2147 Demographic pair Pre-arranged transfer 2476 Demographic pair 2088 Demographic pair; 2089 is ok as an alternative male 3529 Double clutch 3530 Double clutch 21

23 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2530 FT WAYNE F 12 HOLD FT WAYNE 2532 MINNESOTA F 11 HOLD MINNESOTA 2533 MINNESOTA F 11 HOLD MINNESOTA 2536 MEMPHIS F 11 SEND TO CHICAGOLP 2537 DALLAS 08H995 F 11 HOLD DALLAS 2539 JENKINSON JA4751 F 11 HOLD JENKINSON 2540 FORTWORTH F 10 HOLD FORTWORTH 2545 GATLINBAQ Currently genetically overrepresented GB-SD M M 11 HOLD GATLINBAQ 2546 ROCHESTER M 10 HOLD ROCHESTER 2552 LVZOO LV0123 M 10 HOLD LVZOO 2555 ROCHESTER F 10 HOLD ROCHESTER 2557 W ORANGE 6075 F 10 HOLD W ORANGE 2558 MYSTIC SD0503 M 10 SEND TO FT WAYNE 2559 ERIE 1485 F 10 HOLD ERIE 2560 BALTIMORE 4943 F 10 HOLD BALTIMORE 2561 GATLINBAQ GB-SD F F 10 HOLD GATLINBAQ 2562 RACINE M1524 M 10 HOLD RACINE 2565 MINNESOTA F 10 HOLD MINNESOTA 2569 ROCHESTER F 10 HOLD ROCHESTER 2572 MYSTIC SD0602 F 10 HOLD MYSTIC 2575 MYSTIC SD0604 M 10 HOLD MYSTIC 2577 TAMPA AQ M 10 HOLD TAMPA AQ 2578 RACINE M1203 F 10 HOLD RACINE 2579 CHICAGOLP F 10 HOLD CHICAGOLP 2582 TOLEDO 9491 F 10 HOLD TOLEDO 2583 GATLINBAQ GB-SD M M 10 HOLD GATLINBAQ 2586 TAUTPHAUS K6A027 F 10 HOLD TAUTPHAUS 2587 DALLAS WA 13A014 M 10 HOLD DALLAS WA 2589 LOWRY M 10 HOLD LOWRY 2592 MONTERYAQ M 10 HOLD MONTERYAQ 2980 Demographic pair 3328 Double clutch 2061 Demographic pair Demographic pair 2923 Demographic pair 3177 Demographic pair 2474 Demographic Pair Valuable female 22

24 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2593 VANCOUVAQ F 10 HOLD VANCOUVAQ 2595 JENKINSON JA5031 M 10 SEND TO STEINHART 2597 BOS NEAQ F 9 HOLD BOS NEAQ 2600 AQUARAMER FW2 F 9 HOLD AQUARAMER 2601 AQUARAMER S8 M 9 HOLD AQUARAMER 2602 LOWRY F 9 HOLD LOWRY 2605 LITTLEROC 7050 M 9 HOLD LITTLEROC 2606 TAMPA AQ M 9 SEND TO LOWRY 2607 TAMPA AQ M 9 HOLD TAMPA AQ 2610 MEMPHIS M 9 SEND TO CHICAGOLP 2612 LVZOO LV0122 M 9 HOLD LVZOO 2617 TORONTO F 9 HOLD TORONTO 2618 BROOKLYN A08054 F 9 HOLD BROOKLYN 2619 FORTWORTH M 9 HOLD FORTWORTH 2620 TAMPA AQ M 9 HOLD TAMPA AQ 2621 MYSTIC SD0701 F 9 HOLD MYSTIC 2622 TAMPA AQ M 9 HOLD TAMPA AQ 2623 ERIE 1536 M 9 HOLD ERIE 2627 FORTWORTH F 9 HOLD FORTWORTH 2631 LVZOO LV0119 M 9 HOLD LVZOO 2633 STEINHART M 9 HOLD STEINHART 2635 STEINHART F 9 HOLD STEINHART 2638 PUEBLO B27005 F 9 SEND TO CHICAGOLP 2639 LVZOO LV0120 M 9 HOLD LVZOO 2640 BROOKLYN A08053 M 9 HOLD BROOKLYN 2641 BOS NEAQ M 10 HOLD BOS NEAQ 2643 NEWPORTKY F 10 HOLD NEWPORTKY 2644 NEWPORTKY F 10 HOLD NEWPORTKY 2645 NEWPORTKY F 10 HOLD NEWPORTKY 2660 LITTLEROC 7011 M 9 HOLD LITTLEROC 2662 CALDWELL F 9 HOLD CALDWELL 2663 BROOKLYN A11211 F 9 HOLD BROOKLYN Demographic pair 2836 Imprinted but available for AI Pre-arranged transfer Medical/ Potential 2331 Demographic pair Pre-arranged transfer 23

25 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2664 STEINHART F 9 HOLD STEINHART 2665 VANCOUVAQ M 9 HOLD VANCOUVAQ 2666 GREEN NSC M 9 HOLD GREEN NSC 2667 GREEN NSC M 9 HOLD GREEN NSC 2668 STEINHART M 9 HOLD STEINHART 2670 BOS NEAQ F 9 HOLD BOS NEAQ 2672 BALTIMORE UNK M 8 HOLD BALTIMORE 2676 BALTIMORE 5405 F 8 HOLD BALTIMORE 2679 STEINHART F 8 HOLD STEINHART 2680 LITTLEROC 7012 M 8 HOLD LITTLEROC 2681 FT WAYNE F 8 HOLD FT WAYNE 2682 FT WAYNE F 8 HOLD FT WAYNE 2684 LVZOO LV0121 F 8 HOLD LVZOO 2690 OMAHA M 8 HOLD OMAHA 2691 W ORANGE 6112 M 8 HOLD W ORANGE 2692 FT WAYNE M 8 HOLD FT WAYNE 2693 KNOXVILLE 4444 M 8 HOLD KNOXVILLE 2695 FT WAYNE M 8 HOLD FT WAYNE 2697 FT WAYNE M 8 HOLD FT WAYNE 2698 FT WAYNE M 8 HOLD FT WAYNE 2699 RACINE M1523 M 9 HOLD RACINE 2700 LVZOO LV0156 F 9 HOLD LVZOO 2701 TORONTO F 8 HOLD TORONTO 2702 TAUTPHAUS K7A139 F 8 HOLD TAUTPHAUS 2703 CHICAGOLP M 7 HOLD CHICAGOLP 2704 PITTS CA 7731 F 7 HOLD PITTS CA 1933 Demographic pair 3173 Demographic pair 2705 TORONTO M 7 HOLD TORONTO 2706 TORONTO F 7 HOLD TORONTO 2707 GATLINBAQ GB-SD- F 7 HOLD GATLINBAQ F 1629 Demographic pair 2710 TAMPA AQ F 9 HOLD TAMPA AQ 2711 ADVENTURE 3715 F 7 HOLD ADVENTURE 2712 TORONTO M 8 HOLD TORONTO Demographic pair Double clutch; Repair 959 Double clutch

26 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2714 MADISON 2842 M 8 HOLD MADISON 2715 DENVER A08035 M 8 HOLD DENVER 2716 W ORANGE 6123 M 7 HOLD W ORANGE 2717 BROOKLYN A14071 F 7 HOLD BROOKLYN 2719 DENVER A08396 F 7 HOLD DENVER 2720 FT WAYNE F 7 HOLD FT WAYNE 2722 BROOKLYN A10003 F 8 HOLD BROOKLYN 2727 PITTS CA 7576 M 8 HOLD PITTS CA 2730 GATLINBAQ 2731 GATLINBAQ GB-SD M GB-SD F M 8 HOLD GATLINBAQ F 8 HOLD GATLINBAQ 2733 LVZOO LV0117 M 8 HOLD LVZOO 2734 MONTERYAQ F 7 HOLD MONTERYAQ 2737 LITTLEROC 7001 F 7 HOLD LITTLEROC 2741 ROCHESTER M 8 HOLD ROCHESTER 2742 MYSTIC SD0802 M 8 SEND TO MEMPHIS 2744 MYSTIC SD0902 M 7 HOLD MYSTIC 2745 VANCOUVAQ F 8 HOLD VANCOUVAQ 2746 BALTIMORE UNK M 8 HOLD BALTIMORE 2747 VANCOUVAQ F 8 HOLD VANCOUVAQ 2748 AQUARAMER NE4 M 7 SEND TO BUSCH TAM 2749 CHICAGOLP F 7 HOLD CHICAGOLP 2750 BOS NEAQ F 7 HOLD BOS NEAQ 2753 BALTIMORE UNK M 7 HOLD BALTIMORE 2754 BALTIMORE UNK M 7 HOLD BALTIMORE 2755 AQUARAMER P1 M 7 SEND TO BUSCH TAM 2756 LITTLEROC 7014 M 7 NOTES NOTES 2757 OMAHA M 7 HOLD OMAHA 2759 OMAHA F 7 HOLD OMAHA 2764 CHICAGOLP M 7 HOLD CHICAGOLP 2765 JENKINSON JA5836 M 7 HOLD JENKINSON 2766 PITTS CA 7728 F 7 HOLD PITTS CA NOTES 1482 Demographic pair Demographic pair Double clutch 3208 AVAILABLE - SSP is looking for placement 25

27 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2767 MINNESOTA F 7 HOLD MINNESOTA 2768 BALTIMORE 5658 F 7 HOLD BALTIMORE 2769 MINNESOTA M 7 HOLD MINNESOTA 2770 BALTIMORE 5665 F 7 HOLD BALTIMORE 2771 MINNESOTA M 7 HOLD MINNESOTA 2772 NEWPORTKY F 7 HOLD NEWPORTKY 2773 NEWPORTKY F 7 HOLD NEWPORTKY 2775 NEWPORTKY F 7 HOLD NEWPORTKY 2777 MINOT A397 M 7 HOLD MINOT 2778 MONTERYAQ F 7 HOLD MONTERYAQ 2782 MONTERYAQ F 7 HOLD MONTERYAQ 2783 BALTIMORE 5690 M 7 HOLD BALTIMORE 2784 MEMPHIS M 8 HOLD MEMPHIS 2785 LITTLEROC 7049 M 8 HOLD LITTLEROC 2787 TOLEDO 9422 F 7 HOLD TOLEDO 2811 PITTS CA 7723 F 8 HOLD PITTS CA 2815 MEMPHIS F 8 HOLD MEMPHIS 2816 LVZOO LV0159 F 7 HOLD LVZOO 2817 CALDWELL M 7 HOLD CALDWELL 2818 GA AQUAR B09019 M 7 HOLD GA AQUAR 2819 MEMPHIS F 7 HOLD MEMPHIS 2820 MEMPHIS M 7 HOLD MEMPHIS 2822 ROCHESTER M 7 HOLD ROCHESTER 2823 ROCHESTER F 7 HOLD ROCHESTER 2824 GA AQUAR B09020 F 7 HOLD GA AQUAR 2830 BALTIMORE 5983 M 9 HOLD BALTIMORE 2831 LOWRY F 9 HOLD LOWRY 2832 LOWRY M 8 HOLD LOWRY 2833 PITTS CA 7826 M 9 HOLD PITTS CA 2835 LOWRY F 9 HOLD LOWRY 2836 LOWRY F 9 HOLD LOWRY 2837 LOWRY M 9 HOLD LOWRY 3165 Demographic pair 3091 Demographic pair Imprinted/ 2196 Demographic pair Demographic pair; Repair Demographic pair 2784 Demographic pair Double clutch Double clutch 2606 Double clutch

28 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2873 GA AQUAR B09006 F 15 HOLD GA AQUAR 2874 TULSA F 13 HOLD TULSA 2876 GA AQUAR B09009 F 11 HOLD GA AQUAR 2877 GA AQUAR B09001 M 11 HOLD GA AQUAR 2878 GA AQUAR B09002 M 11 HOLD GA AQUAR 2879 GA AQUAR B09003 M 11 HOLD GA AQUAR 2881 GA AQUAR B09004 M 11 HOLD GA AQUAR 2883 BALTIMORE UNK M 7 HOLD BALTIMORE 2884 BALTIMORE UNK M 7 HOLD BALTIMORE 2885 LITTLEROC 7015 M 7 SEND TO TULSA 2886 LITTLEROC 7016 M 7 NOTES NOTES 2888 SPRINGFIE 1023 F 7 HOLD SPRINGFIE 2889 BALTIMORE UNK F 7 HOLD BALTIMORE 2890 ADVENTURE 3896 M 7 HOLD ADVENTURE 2891 ADVENTURE 3906 M 7 HOLD ADVENTURE 2892 ADVENTURE 3907 M 7 HOLD ADVENTURE 2893 LITTLEROC 7017 M 6 HOLD LITTLEROC 2894 PUEBLO B29021 F 6 HOLD PUEBLO 2897 GA AQUAR B09013 F 7 HOLD GA AQUAR 2898 GA AQUAR B09014 M 7 HOLD GA AQUAR 2899 GA AQUAR B09015 M 7 HOLD GA AQUAR 2900 GA AQUAR B09016 M 7 HOLD GA AQUAR 2901 NEWPORTKY F 7 HOLD NEWPORTKY 2902 GATLINBAQ 2903 GATLINBAQ GB-SD M GB-SD M M 7 SEND TO PITTS CA M 7 HOLD GATLINBAQ 2904 NEWPORTKY F 7 HOLD NEWPORTKY 2905 GATLINBAQ GB-SD F F 7 HOLD GATLINBAQ 2906 RACINE M1526 M 7 HOLD RACINE 2908 FT WAYNE M 7 HOLD FT WAYNE 2909 SPRINGFIE 1025 F 6 HOLD SPRINGFIE NOTES Unknown pedigree/ Unknown pedigree/ AVAILABLE - SSP is looking for placement Medical 27

29 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2910 BOS NEAQ M 6 SEND TO W ORANGE 2913 SAGINAW M 6 HOLD SAGINAW 2915 ROCHESTER F 6 HOLD ROCHESTER 2916 MINNESOTA F 6 HOLD MINNESOTA 2921 MINNESOTA M 6 HOLD MINNESOTA 2923 BALTIMORE 5971 M 6 HOLD BALTIMORE 2924 BALTIMORE 5972 F 6 HOLD BALTIMORE 2925 BALTIMORE 5974 F 6 HOLD BALTIMORE 2926 MINNESOTA F 6 HOLD MINNESOTA 2927 MINNESOTA F 6 HOLD MINNESOTA 2928 MINNESOTA F 6 HOLD MINNESOTA 2929 TOLEDO 7822 M 6 HOLD TOLEDO 2931 LITTLEROC 6944 F 6 HOLD LITTLEROC 2932 CINCINNAT F 6 HOLD CINCINNAT 2934 TOLEDO 7823 M 6 HOLD TOLEDO 2935 TOLEDO 7824 M 6 HOLD TOLEDO 2936 MYSTIC SD1001 F 6 HOLD MYSTIC 2937 MYSTIC SD1002 F 6 HOLD MYSTIC double clutch 2939 BOS NEAQ M 6 HOLD BOS NEAQ 2940 VANCOUVAQ M 6 HOLD VANCOUVAQ 2942 VANCOUVAQ F 6 HOLD VANCOUVAQ 2943 BOS NEAQ F 6 HOLD BOS NEAQ 2944 GREEN NSC F 6 HOLD GREEN NSC 2945 BOS NEAQ F 6 HOLD BOS NEAQ 2946 BOS NEAQ M 6 HOLD BOS NEAQ BOS NEAQ M 6 HOLD BOS NEAQ 2948 BOS NEAQ F 6 HOLD BOS NEAQ 1192 Double clutch 2950 LITTLEROC 7048 M 6 HOLD LITTLEROC 2952 TAMPA AQ M 5 HOLD TAMPA AQ 2953 COLUMBUS M 5 HOLD COLUMBUS 2954 COLUMBUS M 5 HOLD COLUMBUS 2955 TULSA M 5 HOLD TULSA Demographic pair Demographic pair

30 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2957 GA AQUAR B10006 F 7 HOLD GA AQUAR 2958 GA AQUAR B10003 F 7 HOLD GA AQUAR 2959 GA AQUAR B10007 M 7 SEND TO MADISON 2961 GA AQUAR B10009 M 7 HOLD GA AQUAR 2962 GA AQUAR B10010 M 7 HOLD GA AQUAR 2963 GA AQUAR B10011 F 7 HOLD GA AQUAR 2964 BALTIMORE 6225 F 7 HOLD BALTIMORE 2965 DALLAS 14P363 F 7 HOLD DALLAS 2966 GA AQUAR B10013 M 7 SEND TO MADISON 2967 TOLEDO 9703 F 7 HOLD TOLEDO 2968 JENKINSON JA6449 F 7 HOLD JENKINSON 2969 GA AQUAR B10019 M 7 HOLD GA AQUAR 2970 GA AQUAR B10015 F 7 SEND TO MADISON 2971 GREENBAY F 7 HOLD GREENBAY 2972 GA AQUAR B10017 F 7 SEND TO MADISON 2973 GA AQUAR B10020 M 8 HOLD GA AQUAR 2974 GA AQUAR B10021 F 7 HOLD GA AQUAR 2975 GA AQUAR B10022 F 6 HOLD GA AQUAR 2977 MINNESOTA M 5 HOLD MINNESOTA 2978 MINNESOTA F 5 HOLD MINNESOTA 2979 GATLINBAQ GB-SD F F 5 HOLD GATLINBAQ 2980 MINNESOTA M 5 HOLD MINNESOTA 2981 BROOKLYN A11212 M 5 HOLD BROOKLYN 2982 BROOKLYN A11213 M 5 HOLD BROOKLYN 2983 STEINHART F 5 HOLD STEINHART 2984 GATLINBAQ 2985 GATLINBAQ 2986 GATLINBAQ 2987 GATLINBAQ 2988 GATLINBAQ 2989 GATLINBAQ GB-SD M GB-SD M GB-SD F GB-SD F GB-SD M GB-SD F M 9 HOLD GATLINBAQ M 8 HOLD GATLINBAQ F 7 HOLD GATLINBAQ F 9 HOLD GATLINBAQ M 7 HOLD GATLINBAQ F 9 HOLD GATLINBAQ 2961 Double clutch Double clutch 2118 Double clutch Double clutch 3102 Double clutch Double clutch; Repair 2415 Double clutch 2959 Double clutch Double clutch Medical Demographic pair; Repair 2375 Demographic pair Double clutch; Repair 2988 Double clutch Double clutch 29

31 ID Location Local ID Sex Age Disposition Location Breeding With Notes 2990 GATLINBAQ 2991 GATLINBAQ 2992 GATLINBAQ 2993 GATLINBAQ 2994 GATLINBAQ 2996 GATLINBAQ GB-SD F GB-SD M GB-SD F GB-SD M GB-SD M GB-SD M F 8 HOLD GATLINBAQ M 8 HOLD GATLINBAQ F 7 HOLD GATLINBAQ M 7 HOLD GATLINBAQ M 6 SEND TO ERIE M 6 HOLD GATLINBAQ 2997 GREEN NSC M 5 HOLD GREEN NSC 2998 PUEBLO B30028 F 5 HOLD PUEBLO 3000 TOLEDO 9427 F 5 HOLD TOLEDO 3001 TULSA F 5 HOLD TULSA 3004 DALLAS 10K275 M 6 HOLD DALLAS 3005 COLUMBUS M 5 HOLD COLUMBUS 3006 COLUMBUS F 5 HOLD COLUMBUS 3007 COLUMBUS M 5 HOLD COLUMBUS 3008 MEMPHIS M 5 HOLD MEMPHIS 3009 COLUMBUS F 5 HOLD COLUMBUS 3010 GREEN NSC F 5 HOLD GREEN NSC 3011 TAUTPHAUS KXA022 M 6 HOLD TAUTPHAUS 3012 TAUTPHAUS KXA023 M 6 HOLD TAUTPHAUS 3013 DALLAS 11K894 F 6 HOLD DALLAS 3014 TAUTPHAUS KXA025 F 6 HOLD TAUTPHAUS 3017 GATLINBAQ 3018 GATLINBAQ Double clutch; Repair Currently genetically overrepresented GB-SD M GB-SD F M 5 HOLD GATLINBAQ F 5 HOLD GATLINBAQ 3019 PITTS CA 8029 F 5 SEND TO LVZOO 3020 DALLAS 11K893 M 5 HOLD DALLAS 3022 BALTIMORE UNK M 6 HOLD BALTIMORE 3023 AQUARAMER D2 M 6 SEND TO BALTIMORE 3025 DENVER A10102 F 6 HOLD DENVER 3027 DENVER A10303 M 5 HOLD DENVER 3030 MYSTIC SD1103 M 5 HOLD MYSTIC 2985 Double clutch 2992 Double clutch 2991 Double clutch Double clutch; 3513 ok as alternative 2935 Demographic pair Double clutch Double clutch; Repair Double clutch; Repair Pre-arranged transfer 30

32 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3040 BALTIMORE 6022 M 5 HOLD BALTIMORE 3041 BALTIMORE 6023 F 5 HOLD BALTIMORE 3043 BALTIMORE 6046 F 5 HOLD BALTIMORE 3044 BALTIMORE 6047 M 5 HOLD BALTIMORE 3045 BALTIMORE 6048 M 5 HOLD BALTIMORE 3046 DALLAS 14P362 M 5 HOLD DALLAS 3050 GREENBAY F 6 HOLD GREENBAY 3053 GREENBAY M 6 SEND TO RACINE 3055 GREENBAY F 6 HOLD GREENBAY 3066 DALLAS WA 12A020 M 5 HOLD DALLAS WA 3067 DALLAS WA 12A021 M 5 HOLD DALLAS WA 3068 COLUMBUS F 5 HOLD COLUMBUS 3070 STEINHART M 5 HOLD STEINHART 3071 ROCHESTER M 5 HOLD ROCHESTER 3073 ADVENTURE 5674 F 5 HOLD ADVENTURE 3074 DALLAS 12M198 F 5 HOLD DALLAS 3075 AQUARAMER D3 M 5 SEND TO BALTIMORE 3079 GREEN NSC F 5 SEND TO ADVENTURE 3080 GREEN NSC F 5 HOLD GREEN NSC 3081 GREEN NSC F 5 HOLD GREEN NSC 3082 GREEN NSC F 5 HOLD GREEN NSC 3083 GREEN NSC M 5 HOLD GREEN NSC 3084 GREEN NSC M 5 HOLD GREEN NSC 3085 GREEN NSC F 5 HOLD GREEN NSC 3086 GREEN NSC F 5 HOLD GREEN NSC 3087 BOS NEAQ F 5 HOLD BOS NEAQ 3090 FORTWORTH F 4 HOLD FORTWORTH 3091 BALTIMORE 6171 M 4 HOLD BALTIMORE 3092 BALTIMORE 6172 M 4 HOLD BALTIMORE NOTES 2370 Demographic pair Demographic pair - may be a low likelihood of success due to female's history of imprinting Alternate breeding female if needed for male 1977 Valuable pair double clutch Currently genetically overrepresented Pre-arranged transfer Paired with sibling Double clutch 2768 Paired with related F

33 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3093 ADVENTURE 5673 M 4 HOLD ADVENTURE 3094 BALTIMORE 6223 M 4 SEND TO ADVENTURE 3096 BALTIMORE 7532 F 4 HOLD BALTIMORE 3097 GREEN NSC M 4 SEND TO ADVENTURE 3100 BUSCH TAM F 4 HOLD BUSCH TAM 3101 GA AQUAR B12003 F 4 HOLD GA AQUAR 3102 JENKINSON JA6450 M 4 HOLD JENKINSON 3103 TOLEDO 9441 F 4 HOLD TOLEDO 3105 PITTS CA 8019 F 4 HOLD PITTS CA 3106 PITTS CA 8020 M 4 HOLD PITTS CA 3107 FT WAYNE F 4 HOLD FT WAYNE 3108 SANDIEGOZ M 4 HOLD SANDIEGOZ 3109 SANDIEGOZ M 4 HOLD SANDIEGOZ 3110 LITTLEROC 7310 M 3 SEND TO GREENBAY 3111 BROOKLYN A12009 F 4 HOLD BROOKLYN 3112 GA AQUAR B12009 F 3 HOLD GA AQUAR 3118 ERIE 2048 M 4 HOLD ERIE 3121 TAMPA AQ F 4 HOLD TAMPA AQ 3122 TAMPA AQ F 4 HOLD TAMPA AQ 3123 DALLAS WA 13A068 M 4 HOLD DALLAS WA 3124 GATLINBAQ 3125 GATLINBAQ GB-SD F GB-SD F F 3 SEND TO ERIE F 3 SEND TO PITTS CA 3126 SAGINAW M 4 HOLD SAGINAW 3127 TOLEDO 9712 F 4 HOLD TOLEDO 3128 ROCHESTER F 4 HOLD ROCHESTER 3129 TAUTPHAUS 14A033 M 3 HOLD TAUTPHAUS 3131 PITTS CA 8634 M 3 HOLD PITTS CA 3132 ADVENTURE 5195 F 3 HOLD ADVENTURE 3133 BOS NEAQ F 4 HOLD BOS NEAQ 3136 PUEBLO B31020 F 4 HOLD PUEBLO NOTES Double clutch; Repair Paired with sibling Double clutch Double clutch; Repair 2934 Double clutch 2994 Demographic pair 2902 Demographic pair 3513 Double clutch; 3510 ok as alternative 32

34 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3137 MEMPHIS F 4 HOLD MEMPHIS 3138 BALTIMORE 7405 F 4 HOLD BALTIMORE 3140 MEMPHIS F 4 HOLD MEMPHIS 3141 MEMPHIS F 3 HOLD MEMPHIS 3142 MEMPHIS F 3 HOLD MEMPHIS 3144 FORTWORTH M 3 HOLD FORTWORTH 3145 TOLEDO 9711 M 3 HOLD TOLEDO 3148 TORONTO F 4 HOLD TORONTO 3151 TORONTO M 4 HOLD TORONTO 3152 TORONTO M 4 HOLD TORONTO 3153 TORONTO M 3 HOLD TORONTO 3165 MINNESOTA M 3 HOLD MINNESOTA 3170 MADISON 2895 F 3 HOLD MADISON 3173 STEINHART M 3 HOLD STEINHART 3174 MEMPHIS 15A124 M 3 HOLD MEMPHIS 3175 CINCINNAT F 4 HOLD CINCINNAT 3176 CINCINNAT M 3 HOLD CINCINNAT 3177 RACINE M1427 M 3 HOLD RACINE 3178 MYSTIC SD1301 F 3 HOLD MYSTIC 3180 MYSTIC SD1303 F 3 HOLD MYSTIC 3187 DALLAS 14P141 M 4 HOLD DALLAS 3188 BROOKLYN A14072 F 4 HOLD BROOKLYN 3189 BOS NEAQ M 4 HOLD BOS NEAQ 3190 DENVER A12029 F 4 HOLD DENVER 3191 DENVER A12139 M 4 HOLD DENVER 3193 TAMPA AQ F 3 HOLD TAMPA AQ 3194 CINCINNAT M 4 SEND TO GREEN NSC 3198 GREENBAY F 3 HOLD GREENBAY 3199 BUSCH TAM M 3 HOLD BUSCH TAM 3200 GA AQUAR B13002 F 3 HOLD GA AQUAR 3201 BUSCH TAM M 3 HOLD BUSCH TAM NOTES 2742 Double clutch 2463 Double clutch; Repair 3008 Demographic pair If he pairs with valuable F 1811, keep fertile eggs 3152 Double clutch Currently genetically overrepresented 2381 Double clutch Double clutch; Repair 3100 Double clutch 33

35 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3202 BUSCH TAM F 3 HOLD BUSCH TAM 3203 GA AQUAR B13006 M 3 HOLD GA AQUAR 3204 GA AQUAR B13007 F 3 HOLD GA AQUAR 3206 FT WAYNE F 3 HOLD FT WAYNE 3207 MINNESOTA M 3 HOLD MINNESOTA 3208 JENKINSON JA6307 F 3 HOLD JENKINSON 3211 GATLINBAQ GB-SD M M 3 HOLD GATLINBAQ 3212 TORONTO M 3 HOLD TORONTO 3213 AQUARAMER A42 F 3 HOLD AQUARAMER 3214 AQUARAMER A43 F 3 HOLD AQUARAMER 3215 ST PAUL 4220 M 3 HOLD ST PAUL 3216 TORONTO M 3 HOLD TORONTO 3217 TORONTO M 3 HOLD TORONTO 3232 BUSCH TAM F 3 HOLD BUSCH TAM 3233 BUSCH TAM M 3 HOLD BUSCH TAM 3235 DENVER A13084 F 3 HOLD DENVER 3236 BROOKLYN A13019 M 3 HOLD BROOKLYN 3237 TAUTPHAUS 13A050 M 3 HOLD TAUTPHAUS 3238 AQUARAMER A45 M 3 HOLD AQUARAMER 3239 AQUARAMER A46 F 3 HOLD AQUARAMER 3240 AQUARAMER A47 M 3 HOLD AQUARAMER 3241 AQUARAMER A48 M 2 HOLD AQUARAMER 3242 AQUARAMER A49 F 2 HOLD AQUARAMER 3245 W ORANGE 6473 F 2 HOLD W ORANGE 3246 AQUARAMER A50 M 2 HOLD AQUARAMER 3247 AQUARAMER A51 F 2 HOLD AQUARAMER 3248 AQUARAMER A52 M 2 HOLD AQUARAMER 3250 BALTIMORE 7317 F 2 HOLD BALTIMORE 3251 BALTIMORE 7318 M 2 HOLD BALTIMORE 3252 BALTIMORE 7322 F 2 HOLD BALTIMORE 3253 BALTIMORE 7329 M 2 HOLD BALTIMORE 3254 BALTIMORE 7330 M 2 HOLD BALTIMORE 3327 Double clutch Double clutch 2765 Double clutch Available pending sire verification 2910 Double clutch 3251 Demographic pair Demographic pair 34

36 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3255 ADVENTURE 5621 F 2 HOLD ADVENTURE 3256 ADVENTURE 5627 M 2 HOLD ADVENTURE 3265 W ORANGE 6474 M 2 HOLD W ORANGE 3266 ROCHESTER F 2 HOLD ROCHESTER 3268 PITTS CA 8629 F 2 HOLD PITTS CA 3269 LITTLEROC 7375 F 2 HOLD LITTLEROC 3272 PITTS CA 8252 F 2 HOLD PITTS CA 3273 PITTS CA 8253 M 2 HOLD PITTS CA 3274 W ORANGE 6475 M 2 HOLD W ORANGE 3275 BALTIMORE 7362 M 2 HOLD BALTIMORE 3278 MINOT A398 M 2 HOLD MINOT 3279 MINNESOTA F 2 HOLD MINNESOTA 3280 MINNESOTA M 2 HOLD MINNESOTA 3281 MINNESOTA F 2 HOLD MINNESOTA 3282 MINNESOTA F 2 HOLD MINNESOTA 3283 MINOT A399 M 2 HOLD MINOT 3284 MINNESOTA M 2 HOLD MINNESOTA 3285 MINNESOTA F 2 HOLD MINNESOTA 3286 MINNESOTA F 2 HOLD MINNESOTA 3287 MINNESOTA F 2 HOLD MINNESOTA 3291 ADVENTURE 5688 M 2 HOLD ADVENTURE 3292 ADVENTURE 5689 F 2 HOLD ADVENTURE 3293 JENKINSON JA6398 M 2 HOLD JENKINSON 3294 GATLINBAQ GG-SD M M 2 HOLD GATLINBAQ 3296 BROOKLYN A13078 F 2 HOLD BROOKLYN 3297 MYSTIC SD1401 F 2 HOLD MYSTIC 3298 MYSTIC SD1402 M 2 HOLD MYSTIC 3299 MYSTIC SD1403 F 2 HOLD MYSTIC 3300 BALTIMORE 7379 F 2 HOLD BALTIMORE Genetically overrepresented currently Genetically overrepresented currently 2893 Demographic pair 3018 Genetically overrepresented currently Genetically overrepresented currently AVAILABLE for indoor exhibits Paired with related M

37 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3301 BALTIMORE 7380 M 2 HOLD BALTIMORE 3302 BALTIMORE 7381 M 2 HOLD BALTIMORE 3303 BALTIMORE 7382 F 2 HOLD BALTIMORE 3304 BALTIMORE 7383 M 2 HOLD BALTIMORE 3306 COLUMBUS M 2 HOLD COLUMBUS 3307 COLUMBUS M 2 HOLD COLUMBUS 3308 GA AQUAR B13009 M 2 HOLD GA AQUAR 3309 GA AQUAR B14001 M 2 HOLD GA AQUAR 3310 GA AQUAR B14002 F 2 HOLD GA AQUAR 3311 GA AQUAR B14003 M 2 HOLD GA AQUAR 3312 GA AQUAR B14004 M 2 HOLD GA AQUAR 3313 GA AQUAR B14007 M 2 HOLD GA AQUAR 3314 JENKINSON JA6434 M 2 HOLD JENKINSON 3315 JENKINSON JA6435 F 2 HOLD JENKINSON 3326 BUSCH TAM M 2 HOLD BUSCH TAM 3327 BUSCH TAM M 2 HOLD BUSCH TAM 3328 BUSCH TAM M 2 SEND TO MINNESOTA 3329 BUSCH TAM F 2 HOLD BUSCH TAM 3333 BROOKLYN A14020 M 2 HOLD BROOKLYN 3334 BROOKLYN A14022 M 2 HOLD BROOKLYN 3338 COLUMBUS F 2 HOLD COLUMBUS 3342 LOWRY F 2 HOLD LOWRY 3343 MONTERYAQ F 2 HOLD MONTERYAQ 3344 MEMPHIS 13A078 M 2 HOLD MEMPHIS 3345 MEMPHIS 13A080 F 2 HOLD MEMPHIS 3347 TAUTPHAUS 14A023 F 1 HOLD TAUTPHAUS 3348 LITTLEROC 7426 F 1 HOLD LITTLEROC 3350 COLO SPRG 2016B0 F 2 SEND TO BROOKLYN 3352 DENVER A14100 M 2 HOLD DENVER 3353 DENVER A14104 F 2 HOLD DENVER imprinted/ potential Medical/ Potential Double clutch; Repair Double clutch; Repair Medical Currently genetically overrepresented 2533 Double clutch 3199 Double clutch 36

38 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3354 TORONTO F 3 HOLD TORONTO 3359 BUSCH TAM M 2 HOLD BUSCH TAM 3361 GREEN NSC M 1 HOLD GREEN NSC 3362 SPRINGFIE 1163 M 1 HOLD SPRINGFIE 3363 FT WAYNE M 1 HOLD FT WAYNE 3365 LITTLEROC 7448 M 1 HOLD LITTLEROC 3366 FT WAYNE M 1 HOLD FT WAYNE 3367 FT WAYNE M 1 HOLD FT WAYNE 3368 BALTIMORE 7515 F 1 HOLD BALTIMORE 3369 BALTIMORE 7521 F 1 HOLD BALTIMORE 3370 ADVENTURE 5955 M 1 HOLD ADVENTURE 3371 GREEN NSC F 1 HOLD GREEN NSC 3373 SANDIEGOZ F 1 HOLD SANDIEGOZ 3374 SANDIEGOZ M 1 HOLD SANDIEGOZ 3377 TULSA M 1 HOLD TULSA 3379 BROOKLYN A14075 M 1 HOLD BROOKLYN 3380 SPRINGFIE 1162 M 1 HOLD SPRINGFIE 3381 GREEN NSC M 1 SEND TO CINCINNAT 3384 BALTIMORE 7567 M 1 HOLD BALTIMORE 3385 GREEN NSC F 1 HOLD GREEN NSC 3386 CALDWELL F 1 HOLD CALDWELL 3388 LOWRY UNK M 1 HOLD LOWRY 3389 PITTS CA 8481 F 1 HOLD PITTS CA 3390 PITTS CA 8485 M 1 HOLD PITTS CA 3391 BALTIMORE 7569 M 1 HOLD BALTIMORE 3395 CHICAGOLP F 2 HOLD CHICAGOLP 3396 BOS NEAQ M 1 HOLD BOS NEAQ 3397 CHICAGOLP M 1 HOLD CHICAGOLP 3398 CHICAGOLP F 1 HOLD CHICAGOLP 3399 ROCHESTER M 1 SEND TO MINOT 3400 ROCHESTER M 1 SEND TO MINOT Genetically overrepresented currently

39 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3401 ROCHESTER M 1 SEND TO MINOT 3402 ROCHESTER F 1 HOLD ROCHESTER 3403 ROCHESTER F 1 SEND TO ADVENTURE 3404 ROCHESTER M 1 SEND TO ADVENTURE 3405 TULSA M 1 HOLD TULSA 3406 TULSA F 1 SEND TO LOWRY 3407 TORONTO M 1 HOLD TORONTO 3408 TORONTO M 1 HOLD TORONTO 3409 TORONTO F 1 HOLD TORONTO 3410 TORONTO F 1 HOLD TORONTO 3411 TORONTO M 1 HOLD TORONTO 3412 COLO SPRG 2016B0 F 2 SEND TO BROOKLYN 3413 DENVER A14195 M 1 HOLD DENVER 3414 DENVER A14216 M 1 HOLD DENVER 3415 DENVER A14233 M 1 HOLD DENVER 3416 GA AQUAR B15002 F 1 HOLD GA AQUAR 3417 BUSCH TAM F 1 HOLD BUSCH TAM 3418 GA AQUAR B15004 M 1 HOLD GA AQUAR 3419 GA AQUAR B15005 F 1 SEND TO BOS NEAQ 3420 LITTLEROC 7474 F 1 HOLD LITTLEROC 3421 LITTLEROC 7475 M 1 HOLD LITTLEROC 3422 BALTIMORE 7576 F 1 HOLD BALTIMORE 3423 BALTIMORE 7580 M 1 HOLD BALTIMORE 3425 TOLEDO F 1 HOLD TOLEDO 3426 BROOKLYN A15014 M 1 HOLD BROOKLYN 3428 DALLAS 15Q117 F 1 HOLD DALLAS 3429 CHICAGOLP M 1 HOLD CHICAGOLP 3430 MADISON 2981 F 1 HOLD MADISON 3431 ST PAUL 4221 F 1 HOLD ST PAUL 3432 PUEBLO B36001 M 1 HOLD PUEBLO 3433 JENKINSON JA 656 M 1 HOLD JENKINSON 3434 JENKINSON JA 657 M 1 HOLD JENKINSON Pair with Double clutch 3396 Double clutch Genetically valuable Valuable AVAILABLE for indoor exhibits 38

40 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3436 FORTWORTH F 1 HOLD FORTWORTH 3437 FORTWORTH M 1 HOLD FORTWORTH 3438 FORTWORTH F 1 HOLD FORTWORTH 3439 FORTWORTH M 1 HOLD FORTWORTH 3440 DALLAS 15Q278 F 1 HOLD DALLAS 3441 DALLAS 15Q279 F 1 HOLD DALLAS 3442 CHICAGOLP M 1 HOLD CHICAGOLP 3445 DALLAS 15Q280 F 1 HOLD DALLAS 3450 MONTERYAQ M 1 HOLD MONTERYAQ 3451 GA AQUAR B15006 F 1 HOLD GA AQUAR 3452 GA AQUAR B15007 F 1 HOLD GA AQUAR 3453 GA AQUAR B15008 M 1 HOLD GA AQUAR 3454 GA AQUAR B15009 M 1 HOLD GA AQUAR 3455 GA AQUAR B15010 M 1 HOLD GA AQUAR 3458 CHICAGOLP M 0 HOLD CHICAGOLP 3459 CHICAGOLP M 0 HOLD CHICAGOLP 3460 AQUARAMER A54 F 0 HOLD AQUARAMER 3463 DENVER A15174 M 0 HOLD DENVER 3464 DENVER A15185 F 0 HOLD DENVER 3465 DENVER A15222 M 0 HOLD DENVER 3466 ROCHESTER F 0 SEND TO LVZOO 3467 ROCHESTER M 0 SEND TO ADVENTURE 3468 BOS NEAQ M 1 HOLD BOS NEAQ 3469 BOS NEAQ M 1 HOLD BOS NEAQ 3470 CHICAGOLP M 1 HOLD CHICAGOLP 3471 BOS NEAQ F 1 SEND TO GA AQUAR 3475 BALTIMORE 7852 M 0 HOLD BALTIMORE 3476 BALTIMORE 7853 F 0 HOLD BALTIMORE 3477 TULSA M 0 HOLD TULSA 3478 GREEN NSC F 0 HOLD GREEN NSC 3046 Demographic pair Currently genetically overrepresented Currently genetically overrepresented 3203 Double clutch

41 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3479 BALTIMORE 7855 M 0 HOLD BALTIMORE 3480 BROOKLYN A15101 M 0 HOLD BROOKLYN 3481 TOLEDO F 0 SEND TO SANDIEGOZ 3482 TOLEDO M 0 SEND TO SANDIEGOZ 3484 FT WAYNE F 0 HOLD FT WAYNE 3485 ROCHESTER U 0 HOLD ROCHESTER 3486 MINNESOTA F 0 HOLD MINNESOTA 3487 MINNESOTA M 0 HOLD MINNESOTA 3488 MINNESOTA M 0 HOLD MINNESOTA 3490 BALTIMORE 7868 F 0 HOLD BALTIMORE 3491 MEMPHIS 15A118 M 0 HOLD MEMPHIS 3492 MEMPHIS 15A119 M 0 HOLD MEMPHIS 3493 MEMPHIS 15A120 M 0 HOLD MEMPHIS 3501 STEINHART M 0 HOLD STEINHART 3502 ROCHESTER U 0 HOLD ROCHESTER 3503 RACINE M1601 F 0 HOLD RACINE 3504 RACINE M1602 M 0 HOLD RACINE 3506 TOLEDO F 0 HOLD TOLEDO 3508 ADVENTURE 6260 F 0 HOLD ADVENTURE 3510 DALLAS 16T009 M 0 SEND TO PUEBLO 3511 BALTIMORE 7870 M 0 HOLD BALTIMORE 3512 FT WAYNE M 0 HOLD FT WAYNE 3513 DALLAS 16T011 M 0 SEND TO PUEBLO 3515 FORTWORTH F 1 HOLD FORTWORTH 3516 FORTWORTH M 0 HOLD FORTWORTH 3519 MYSTIC SD1504 F 0 HOLD MYSTIC 3520 MYSTIC SD1505 M 0 HOLD MYSTIC 3521 MYSTIC SD1601 F 0 HOLD MYSTIC 3522 MYSTIC SD1602 M 0 HOLD MYSTIC ; Pre-arranged transfer for fall 2016 Pre-arranged transfer for fall 2016 Genetically overrepresented currently 2998 Double clutch 3136 Double clutch 40

42 ID Location Local ID Sex Age Disposition Location Breeding With Notes 3523 MINNESOTA M 0 HOLD MINNESOTA 3524 MINNESOTA F 0 HOLD MINNESOTA 3526 TULSA F 0 HOLD TULSA 3527 BALTIMORE 7880 M 0 HOLD BALTIMORE 3528 GREEN NSC M 0 HOLD GREEN NSC 3529 BUSCH TAM M 1 HOLD BUSCH TAM 3530 BUSCH TAM M 1 SEND TO SAGINAW 3531 GA AQUAR B16001 M 0 HOLD GA AQUAR 3532 GA AQUAR B16002 M 0 HOLD GA AQUAR 3560 MINNESOTA F 0 HOLD MINNESOTA 3561 MINNESOTA F 0 HOLD MINNESOTA 3562 BALTIMORE 7933 M 0 HOLD BALTIMORE 3563 BALTIMORE 7934 F 0 HOLD BALTIMORE 3566 MINNESOTA M 0 HOLD MINNESOTA 3568 MONTERYAQ M 0 HOLD MONTERYAQ 3569 STEINHART M 0 HOLD STEINHART 3570 STEINHART M 0 HOLD STEINHART 3573 TAUTPHAUS 16A017 U 0 HOLD TAUTPHAUS 3574 ADVENTURE 6367 F 0 HOLD ADVENTURE 3575 ADVENTURE 6374 M 0 HOLD ADVENTURE 3576 DENVER A16077 M 0 HOLD DENVER Genetically overrepresented currently Genetically overrepresented currently 41

43 ADVENTURE Adventure Aquarium Breeding and Transfer Recommendations by Institution ( animals =, Available animals = ) 1 Riverside Drive, Camden, NJ , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD ADVENTURE F HOLD ADVENTURE 973 Valuable pair - double clutch; lower likelihood of success (have not been producing fertile eggs) F HOLD ADVENTURE F HOLD ADVENTURE 2077 Demographic pair M HOLD ADVENTURE M??? 18 HOLD ADVENTURE Potential F HOLD ADVENTURE 2408 Demographic pair M??? 14 HOLD ADVENTURE Potential M HOLD ADVENTURE F HOLD ADVENTURE F HOLD ADVENTURE M??? 7 HOLD ADVENTURE Medical M HOLD ADVENTURE M HOLD ADVENTURE F HOLD ADVENTURE 3093 Valuable pair - double clutch F RECEIVE FROM GREEN NSC Paired with sibling M HOLD ADVENTURE M RECEIVE FROM BALTIMORE M RECEIVE FROM GREEN NSC F??? 3 HOLD ADVENTURE F HOLD ADVENTURE M HOLD ADVENTURE M HOLD ADVENTURE F HOLD ADVENTURE M HOLD ADVENTURE F M M RECEIVE FROM RECEIVE FROM RECEIVE FROM ROCHESTER ROCHESTER ROCHESTER F HOLD ADVENTURE F HOLD ADVENTURE M HOLD ADVENTURE Current holdings: 25 ( ); Holdings following transfers: 31 ( ) Potential Genetically overrepresented currently Genetically overrepresented currently Genetically overrepresented currently Genetically overrepresented currently Genetically overrepresented currently Genetically overrepresented currently Genetically overrepresented currently Genetically overrepresented currently 42

44 AQUARAMER Audubon Aquarium of the Americas 1 Canal St., New Orleans, LA , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes 526 S3 M??? 34 HOLD AQUARAMER Age 1111 R3 M??? 27 HOLD AQUARAMER Age/ Medical 1220 R7 M HOLD AQUARAMER 1240 S6 F??? 26 HOLD AQUARAMER Age 1299 S5 F??? 26 HOLD AQUARAMER Age/ Medical 1448 A1 F??? 25 HOLD AQUARAMER Age/Education 2357 FW1 M??? 15 HOLD AQUARAMER Potential 2600 FW2 F HOLD AQUARAMER 2601 S8 M HOLD AQUARAMER Imprinted but available for AI 2748 NE4 M SEND TO BUSCH TAM 2755 P1 M SEND TO BUSCH TAM 3023 D2 M SEND TO BALTIMORE Pre-arranged transfer 3075 D3 M SEND TO BALTIMORE Pre-arranged transfer 3213 A42 F HOLD AQUARAMER 3214 A43 F HOLD AQUARAMER 3238 A45 M HOLD AQUARAMER 3239 A46 F HOLD AQUARAMER 3240 A47 M HOLD AQUARAMER 3241 A48 M??? 2 HOLD AQUARAMER Potential 3242 A49 F??? 2 HOLD AQUARAMER 3246 A50 M HOLD AQUARAMER 3247 A51 F HOLD AQUARAMER 3248 A52 M HOLD AQUARAMER 3460 A54 F HOLD AQUARAMER Potential Current holdings: 24 ( ); Holdings following transfers: 20 ( ) 43

45 BALTIMORE Maryland Zoo in Baltimore 1876 Mansion House Drive, Baltimore, MD 21217, USA Institution Notes: If space allows, use the MateRx on the next page to identify additional breeding pairs to those that have already been recommended. MSIs of 1, 2, or 3 should be prioritized and may double clutch, while pairs MSIs of 5, 6, or - should not be bred. Some MSIs of 4 may be necessary if better ranked pairs are unavailable or unsuccessful. Contact the SSP coordinator first for approval to breed any potential pairs using the MateRx matrix. ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD BALTIMORE F??? 29 HOLD BALTIMORE Age M HOLD BALTIMORE F HOLD BALTIMORE F HOLD BALTIMORE M HOLD BALTIMORE 1672 UNK M HOLD BALTIMORE F??? 19 HOLD BALTIMORE Double clutch, though low likelihood of success expected due to age Potential M HOLD BALTIMORE F HOLD BALTIMORE 1672 Demographic pair F HOLD BALTIMORE M HOLD BALTIMORE M HOLD BALTIMORE UNK M HOLD BALTIMORE B022 M HOLD BALTIMORE Genetically valuable B038 M HOLD BALTIMORE F??? 15 HOLD BALTIMORE F HOLD BALTIMORE M HOLD BALTIMORE M HOLD BALTIMORE 2104 Potential Paired with related male 2333 Paired with related female F HOLD BALTIMORE 3045 Demographic pair; Male 2147 is ok as an alternative 2373 UNK F HOLD BALTIMORE F SEND TO LVZOO F HOLD BALTIMORE 2746 Demographic pair M HOLD BALTIMORE F HOLD BALTIMORE 2473 Double clutch F HOLD BALTIMORE 2147 Demographic pair M HOLD BALTIMORE M HOLD BALTIMORE F HOLD BALTIMORE 2923 Demographic pair 2672 UNK M HOLD BALTIMORE F HOLD BALTIMORE 2746 UNK M HOLD BALTIMORE UNK M HOLD BALTIMORE 2754 UNK M HOLD BALTIMORE F HOLD BALTIMORE 3091 Demographic pair F??? 7 HOLD BALTIMORE Imprinted/ M??? 7 HOLD BALTIMORE M HOLD BALTIMORE UNK M HOLD BALTIMORE 2884 UNK M HOLD BALTIMORE 2889 UNK F HOLD BALTIMORE Potential 44

46 ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD BALTIMORE F HOLD BALTIMORE F??? 6 HOLD BALTIMORE Potential F HOLD BALTIMORE 3022 UNK M HOLD BALTIMORE 3023 D2 M RECEIVE FROM AQUARAMER Pre-arranged transfer M??? 5 HOLD BALTIMORE F HOLD BALTIMORE F HOLD BALTIMORE Potential M??? 5 HOLD BALTIMORE Potential M HOLD BALTIMORE 2370 Demographic pair RECEIVE 3075 D3 M AQUARAMER Pre-arranged transfer FROM M HOLD BALTIMORE 2768 Paired with related M HOLD BALTIMORE female M SEND TO ADVENTURE F HOLD BALTIMORE 2830 Double clutch; Repair F HOLD BALTIMORE 2463 Double clutch; Repair F HOLD BALTIMORE 3251 Demographic pair M HOLD BALTIMORE F HOLD BALTIMORE 3301 Demographic pair M??? 2 HOLD BALTIMORE M??? 2 HOLD BALTIMORE M??? 2 HOLD BALTIMORE F HOLD BALTIMORE M HOLD BALTIMORE M??? 2 HOLD BALTIMORE F??? 2 HOLD BALTIMORE M??? 2 HOLD BALTIMORE F??? 1 HOLD BALTIMORE F HOLD BALTIMORE M HOLD BALTIMORE M??? 1 HOLD BALTIMORE F HOLD BALTIMORE M HOLD BALTIMORE M HOLD BALTIMORE F HOLD BALTIMORE M HOLD BALTIMORE F??? 0 HOLD BALTIMORE M HOLD BALTIMORE M??? 0 HOLD BALTIMORE M??? 0 HOLD BALTIMORE F HOLD BALTIMORE Potential Potential Potential Paired with related M 3092 Potential imprinted/ potential Potential Potential Potential Potential Potential Potential Current holdings: 82 ( ); Holdings following transfers: 82 ( ) 45

47 Males > Draft for Review by Institutional Representatives Please comment by 21 Oct 2016 BALTIMORE 2016 MateRx Females > ,2,3. 4s OK too. 5,6,"-" BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE 3023 AQUARAMER AQUARAMER BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE BALTIMORE 46

48 BOS NEAQ New England Aquarium Central Wharf, Boston, MA , USA Institution Notes: If space allows, use the MateRx on the next page to identify additional breeding pairs to those that have already been recommended. MSIs of 1, 2, or 3 should be prioritized and may double clutch, while pairs MSIs of 5, 6, or - should not produce offspring. Some MSIs of 4 may be necessary if better ranked pairs are unavailable or unsuccessful. Contact the SSP coordinator first for approval to breed any potential pairs using the MateRx matrix. ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 34 HOLD BOS NEAQ Age F??? 32 HOLD BOS NEAQ Age M HOLD BOS NEAQ M HOLD BOS NEAQ F HOLD BOS NEAQ M HOLD BOS NEAQ 2464 Double clutch, though low likelihood of success expected due to age F HOLD BOS NEAQ 2946 Double clutch M HOLD BOS NEAQ F HOLD BOS NEAQ M HOLD BOS NEAQ M HOLD BOS NEAQ M HOLD BOS NEAQ F HOLD BOS NEAQ 2066 Demographic pair M HOLD BOS NEAQ M HOLD BOS NEAQ F HOLD BOS NEAQ F HOLD BOS NEAQ M HOLD BOS NEAQ M HOLD BOS NEAQ F HOLD BOS NEAQ M HOLD BOS NEAQ F HOLD BOS NEAQ F HOLD BOS NEAQ Valuable but some relatedness - one clutch per year Double clutch; Female 2419 ok as an alternative F HOLD BOS NEAQ 1571 Double clutch F HOLD BOS NEAQ 2641 Demographic pair M HOLD BOS NEAQ F HOLD BOS NEAQ 1933 Demographic pair F HOLD BOS NEAQ 1688 Double clutch M SEND TO W ORANGE M HOLD BOS NEAQ F HOLD BOS NEAQ F HOLD BOS NEAQ M HOLD BOS NEAQ M HOLD BOS NEAQ 47

49 Males > Draft for Review by Institutional Representatives Please comment by 21 Oct 2016 ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD BOS NEAQ 1192 Double clutch F HOLD BOS NEAQ F HOLD BOS NEAQ M??? 4 HOLD BOS NEAQ Potential M HOLD BOS NEAQ B15005 F RECEIVE FROM GA AQUAR 3396 Double clutch M HOLD BOS NEAQ M HOLD BOS NEAQ F SEND TO GA AQUAR 3453 Current holdings: 42 ( ); Holdings following transfers: 42 ( ) BOS NEAQ 2016 MateRx Females > ,2,3. 4s OK too. DO NOT 5,6,"-" BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ GA AQUAR 1192 BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ BOS NEAQ

50 BROOKLYN New York Aquarium Surf Avenue at West 8th Street, Brooklyn, NY , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD BROOKLYN M HOLD BROOKLYN F HOLD BROOKLYN A006 M RECEIVE FROM COLO SPRG A007 F RECEIVE FROM COLO SPRG 2157 Double clutch 1913 A10002 F HOLD BROOKLYN 2052 A11210 F HOLD BROOKLYN M HOLD BROOKLYN A009 M RECEIVE FROM COLO SPRG A010 M RECEIVE FROM COLO SPRG A011 M RECEIVE FROM COLO SPRG A015 M RECEIVE FROM COLO SPRG A016 F RECEIVE FROM COLO SPRG 2618 A08054 F HOLD BROOKLYN 2640 A08053 M??? 9 HOLD BROOKLYN 2663 A11211 F??? 9 HOLD BROOKLYN 2717 A14071 F HOLD BROOKLYN Demographic pair; Repair 2722 A10003 F HOLD BROOKLYN 1482 Demographic pair 2981 A11212 M HOLD BROOKLYN 2982 A11213 M HOLD BROOKLYN A12009 F??? 4 HOLD BROOKLYN 3188 A14072 F??? 4 HOLD BROOKLYN 3236 A13019 M??? 3 HOLD BROOKLYN 3296 A13078 F??? 2 HOLD BROOKLYN 3333 A14020 M??? 2 HOLD BROOKLYN 3334 A14022 M??? 2 HOLD BROOKLYN B0 F RECEIVE FROM COLO SPRG 3379 A14075 M??? 1 HOLD BROOKLYN B0 F RECEIVE FROM COLO SPRG 3426 A15014 M??? 1 HOLD BROOKLYN 3480 A15101 M??? 0 HOLD BROOKLYN Demographic pair; Repair Current holdings: 22 ( ); Holdings following transfers: 31 ( ) 49

51 BUSCH TAM Busch Gardens Tampa Bay 3605 Bougainvillea Ave., Tampa, FL 33612, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F RECEIVE FROM SAGINAW 3529 Double clutch 2748 NE4 M RECEIVE FROM AQUARAMER 2755 P1 M RECEIVE FROM AQUARAMER F HOLD BUSCH TAM 3201 Double clutch M HOLD BUSCH TAM M HOLD BUSCH TAM 3100 Double clutch F HOLD BUSCH TAM 3327 Double clutch F HOLD BUSCH TAM M HOLD BUSCH TAM M HOLD BUSCH TAM M HOLD BUSCH TAM 3202 Currently genetically overrepresented M SEND TO MINNESOTA 2533 Double clutch F HOLD BUSCH TAM 3199 Double clutch M??? 2 HOLD BUSCH TAM F HOLD BUSCH TAM 3233 Double clutch M HOLD BUSCH TAM M SEND TO SAGINAW 2508 Current holdings: 14 (9.5.0); Holdings following transfers: 15 (9.6.0) CALDWELL Caldwell Zoo PO Box 4785, Tyler, TX , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD CALDWELL F HOLD CALDWELL 2367 Demographic pair M HOLD CALDWELL M HOLD CALDWELL M HOLD CALDWELL F HOLD CALDWELL 2817 Demographic pair F HOLD CALDWELL 2445 Double clutch M HOLD CALDWELL F??? 9 HOLD CALDWELL M HOLD CALDWELL F HOLD CALDWELL Potential Current holdings: 11 (6.5.0); Holdings following transfers: 11 (6.5.0) 50

52 CHICAGOLP Lincoln Park Zoological Gardens PO Box 14903, Chicago, IL , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes 2474 B25023 M RECEIVE FROM PUEBLO 2579 Pre-arranged transfer F RECEIVE FROM MEMPHIS F HOLD CHICAGOLP 2474 Demographic Pair M RECEIVE FROM MEMPHIS 2638 B27005 F RECEIVE FROM PUEBLO Pre-arranged M??? 7 HOLD CHICAGOLP F HOLD CHICAGOLP M HOLD CHICAGOLP F HOLD CHICAGOLP M??? 1 HOLD CHICAGOLP F??? 1 HOLD CHICAGOLP M??? 1 HOLD CHICAGOLP M??? 1 HOLD CHICAGOLP M HOLD CHICAGOLP M HOLD CHICAGOLP M HOLD CHICAGOLP Current holdings: 12 (8.4.0); Holdings following transfers: 16 (10.6.0) transfer CINCINNAT Cincinnati Zoo & Botanical Garden 3400 Vine St., Cincinnati, OH , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 31 HOLD CINCINNAT Age F HOLD CINCINNAT F HOLD CINCINNAT M HOLD CINCINNAT M HOLD CINCINNAT M HOLD CINCINNAT M HOLD CINCINNAT M HOLD CINCINNAT F HOLD CINCINNAT F HOLD CINCINNAT M HOLD CINCINNAT M SEND TO GREEN NSC M RECEIVE FROM GREEN NSC Current holdings: 12 (8.4.0); Holdings following transfers: 12 (8.4.0) 51

53 COLO SPRG Cheyenne Mtn Zoological Park 4250 Cheyenne Mountain Zoo Rd., Colorado Springs, CO 80906, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes A006 M SEND TO BROOKLYN A007 F SEND TO BROOKLYN 2157 Double clutch A009 M SEND TO BROOKLYN A010 M SEND TO BROOKLYN A011 M SEND TO BROOKLYN A015 M SEND TO BROOKLYN A016 F SEND TO BROOKLYN B0 F SEND TO BROOKLYN B0 F SEND TO BROOKLYN Current holdings: 9 (5.4.0); Holdings following transfers: 0 (0.0.0) COLUMBUS Columbus Zoo and Aquarium PO Box 400, Powell, OH , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD COLUMBUS F HOLD COLUMBUS M HOLD COLUMBUS M HOLD COLUMBUS M HOLD COLUMBUS F??? 5 HOLD COLUMBUS M HOLD COLUMBUS F??? 5 HOLD COLUMBUS F HOLD COLUMBUS M??? 2 HOLD COLUMBUS M??? 2 HOLD COLUMBUS F??? 2 HOLD COLUMBUS Current holdings: 12 (7.5.0); Holdings following transfers: 12 (7.5.0) Potential Potential Potential Potential Potential 52

54 DALLAS Dallas Zoo 650 South R.L. Thornton Freeway, Dallas, TX , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes G547 M HOLD DALLAS G548 F SEND TO MEMPHIS 2388 Double clutch 1784 B32011 M??? 22 RECEIVE FROM PUEBLO Potential G552 M HOLD DALLAS H995 F HOLD DALLAS Currently genetically over-represented P363 F HOLD DALLAS 2118 Double clutch K275 M HOLD DALLAS Currently genetically over-represented K894 F??? 6 HOLD DALLAS Potential K893 M??? 5 HOLD DALLAS Potential P362 M HOLD DALLAS M198 F HOLD DALLAS Currently genetically P141 M HOLD DALLAS Q117 F HOLD DALLAS over-represented Currently genetically over-represented Valuable Q278 F HOLD DALLAS 3046 Demographic pair Q279 F HOLD DALLAS Currently genetically over-represented Q280 F HOLD DALLAS Currently genetically over-represented T009 M SEND TO PUEBLO 2998 Double clutch T011 M SEND TO PUEBLO 3136 Double clutch Current holdings: 17 (8.9.0); Holdings following transfers: 15 (7.8.0) DALLAS WA Dallas World Aquarium 1801 N. Griffin St., Dallas, TX , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes A034 M HOLD DALLAS WA A032 M??? 11 HOLD DALLAS WA A014 M HOLD DALLAS WA A020 M HOLD DALLAS WA A021 M HOLD DALLAS WA A068 M HOLD DALLAS WA Current holdings: 17 (8.9.0); Holdings following transfers: 17 (8.9.0) Potential 53

55 DENVER Denver Zoological Gardens City Park, Denver, CO 80205, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 32 HOLD DENVER Age 1477 A06140 F??? 24 HOLD DENVER 2451 A05444 F HOLD DENVER 2476 A04169 M HOLD DENVER A05445 F HOLD DENVER 2476 Demographic pair 2715 A08035 M??? 8 HOLD DENVER 2719 A08396 F??? 7 HOLD DENVER 3025 A10102 F HOLD DENVER 3027 A10303 M??? 5 HOLD DENVER 3190 A12029 F HOLD DENVER 3191 A12139 M HOLD DENVER 3235 A13084 F HOLD DENVER 3352 A14100 M HOLD DENVER 3353 A14104 F HOLD DENVER 3413 A14195 M HOLD DENVER 3414 A14216 M HOLD DENVER 3415 A14233 M HOLD DENVER 3463 A15174 M HOLD DENVER 3464 A15185 F HOLD DENVER 3465 A15222 M??? 0 HOLD DENVER 3576 A16077 M??? 0 HOLD DENVER Current holdings: 21 (12.9.0); Holdings following transfers: 21 (12.9.0) ERIE Erie Zoological Gardens 423 W 38th St, Erie, PA , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD ERIE M HOLD ERIE M HOLD ERIE M HOLD ERIE F HOLD ERIE F HOLD ERIE F HOLD ERIE M HOLD ERIE GB-SD- RECEIVE 2994 M GATLINBAQ M FROM M HOLD ERIE GB-SD- RECEIVE 3124 F GATLINBAQ F FROM Current holdings: 9 (5.4.0); Holdings following transfers: 11 (6.5.0) Demographic pair Demographic pair 54

56 FORTWORTH Fort Worth Zoological Park 1989 Colonial Pkwy., Ft Worth, TX , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 32 HOLD FORTWORTH Age M??? 26 HOLD FORTWORTH Potential F HOLD FORTWORTH F HOLD FORTWORTH 1815 Double clutch F HOLD FORTWORTH NOTES Valuable older female - priority for breeding. If she pairs with 2337, 2396, or 3144, keep fertile eggs M HOLD FORTWORTH F??? 17 HOLD FORTWORTH Potential M HOLD FORTWORTH M HOLD FORTWORTH 2397 If he pairs with valuable older female 1811, keep fertile eggs M HOLD FORTWORTH NOTES If he pairs with valuable older female 1811, keep fertile eggs F HOLD FORTWORTH 2337 Double clutch F HOLD FORTWORTH F HOLD FORTWORTH M HOLD FORTWORTH F HOLD FORTWORTH 2331 Demographic pair F HOLD FORTWORTH M HOLD FORTWORTH NOTES F HOLD FORTWORTH M HOLD FORTWORTH F HOLD FORTWORTH M HOLD FORTWORTH F HOLD FORTWORTH M HOLD FORTWORTH Current holdings: 23 ( ); Holdings following transfers: 23 ( ) If he pairs with valuable older female 1811, keep fertile eggs 55

57 FT WAYNE Fort Wayne Children's Zoo 3411 Sherman Blvd., Fort Wayne, IN , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD FT WAYNE F HOLD FT WAYNE M HOLD FT WAYNE F HOLD FT WAYNE 2558 Double clutch M HOLD FT WAYNE F HOLD FT WAYNE 2558 SD0503 M RECEIVE FROM MYSTIC F HOLD FT WAYNE F HOLD FT WAYNE M HOLD FT WAYNE M HOLD FT WAYNE M HOLD FT WAYNE M HOLD FT WAYNE F HOLD FT WAYNE M HOLD FT WAYNE F HOLD FT WAYNE F HOLD FT WAYNE 2171 Double clutch M HOLD FT WAYNE M HOLD FT WAYNE M HOLD FT WAYNE F HOLD FT WAYNE M HOLD FT WAYNE Current holdings: 21 (12.9.0); Holdings following transfers: 22 (13.9.0) 56

58 GA AQUAR Georgia Aquarium 225 Baker Street, Atlanta, GA 30313, USA Institution Notes: If space allows, use the MateRx on the next page to identify additional breeding pairs to those that have already been recommended. MSIs of 1, 2, or 3 should be prioritized and may double clutch, while pairs with MSIs of 5, 6, or - are not recommended. Some MSIs of 4 may be necessary if better ranked pairs are unavailable or unsuccessful. Contact the SSP coordinator first for approval to breed any potential pairs using the MateRx matrix. ID Local ID Sex MK Age Disposition Location Breeding With Notes 840 B09017 M??? 30 HOLD GA AQUAR Age 937 B09018 F??? 29 HOLD GA AQUAR Age 1147 B10002 F??? 27 HOLD GA AQUAR Age 1545 B10001 M HOLD GA AQUAR 1820 B09011 F??? 22 HOLD GA AQUAR 2818 B09019 M HOLD GA AQUAR 2824 B09020 F HOLD GA AQUAR 2873 B09006 F??? 15 HOLD GA AQUAR 2876 B09009 F??? 11 HOLD GA AQUAR 2877 B09001 M??? 11 HOLD GA AQUAR 2878 B09002 M??? 11 HOLD GA AQUAR 2879 B09003 M??? 11 HOLD GA AQUAR 2881 B09004 M??? 11 HOLD GA AQUAR 2897 B09013 F HOLD GA AQUAR 2898 B09014 M HOLD GA AQUAR 2899 B09015 M??? 7 HOLD GA AQUAR Potential Potential Potential Unknown pedigree/ Potential Potential Unknown pedigree/ Potential Potential Potential 2900 B09016 M HOLD GA AQUAR 2957 B10006 F HOLD GA AQUAR 2958 B10003 F HOLD GA AQUAR 2961 Double clutch 2959 B10007 M SEND TO MADISON B10009 M HOLD GA AQUAR B10010 M HOLD GA AQUAR 2963 B10011 F HOLD GA AQUAR 2969 Double clutch 2966 B10013 M SEND TO MADISON B10019 M HOLD GA AQUAR B10015 F SEND TO MADISON 2966 Double clutch; Repair 2972 B10017 F SEND TO MADISON 2959 Double clutch 2973 B10020 M HOLD GA AQUAR B10021 F HOLD GA AQUAR 2973 Double clutch 2975 B10022 F??? 6 HOLD GA AQUAR Medical 3101 B12003 F HOLD GA AQUAR 3312 Double clutch; Repair 3112 B12009 F HOLD GA AQUAR 3200 B13002 F HOLD GA AQUAR 3311 Double clutch; Repair 3203 B13006 M HOLD GA AQUAR B13007 F??? 3 HOLD GA AQUAR 3308 B13009 M HOLD GA AQUAR 3310 Potential 3309 B14001 M??? 2 HOLD GA AQUAR Medical/ 3310 B14002 F HOLD GA AQUAR 3308 Double clutch; Repair 3311 B14003 M HOLD GA AQUAR 3200 Double clutch; Repair 3312 B14004 M HOLD GA AQUAR B14007 M??? 2 HOLD GA AQUAR Medical 57

59 Males > Draft for Review by Institutional Representatives Please comment by 21 Oct 2016 ID Local ID Sex MK Age Disposition Location Breeding With Notes 3416 B15002 F HOLD GA AQUAR 3418 B15004 M HOLD GA AQUAR 3419 B15005 F SEND TO BOS NEAQ 3396 Double clutch 3451 B15006 F HOLD GA AQUAR 3203 Double clutch 3452 B15007 F??? 1 HOLD GA AQUAR 3453 B15008 M HOLD GA AQUAR B15009 M HOLD GA AQUAR 3455 B15010 M??? 1 HOLD GA AQUAR 3531 B16001 M HOLD GA AQUAR 3532 B16002 M??? 0 HOLD GA AQUAR F RECEIVE FROM Current holdings: 51 ( ); Holdings following transfers: 47 ( ) Potential Potential Potential BOS NEAQ 3453 Double clutch Females > GA AQUAR 2016 MateRx ,2,3. 4s OK too. 5,6,"-" 1545 GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR GA AQUAR BOS NEAQ GA AQUAR GA AQUAR GA AQUAR 2958 GA AQUAR 2963 GA AQUAR 2974 GA AQUAR 3101 GA AQUAR 3112 GA AQUAR 3200 GA AQUAR 3310 GA AQUAR 3416 GA AQUAR 3451 GA AQUAR 58

60 GATLINBAQ Ripley's Aquarium of the Smokies 88 River Road, Gatlinburg, TN 37788, USA Institution Notes: If space allows, use the MateRx on the next page to identify additional breeding pairs to those that have already been recommended. MSIs of 1, 2, or 3 should be prioritized and may double clutch, while pairs with MSIs of 5, 6, or - are not recommended. Some MSIs of 4 may be necessary if better ranked pairs are unavailable or unsuccessful. Contact the SSP coordinator first for approval to breed any potential pairs using the MateRx matrix. ID Local ID Sex MK Age Disposition Location Breeding With Notes 1629 GB-SD M M HOLD GATLINBAQ GB-SD F F HOLD GATLINBAQ 2101 GB-SD M M HOLD GATLINBAQ 2545 GB-SD M M HOLD GATLINBAQ 2561 GB-SD F F HOLD GATLINBAQ 2583 GB-SD M M HOLD GATLINBAQ 2707 GB-SD F F HOLD GATLINBAQ 1629 Demographic pair 2730 GB-SD M M??? 8 HOLD GATLINBAQ 2731 GB-SD F F HOLD GATLINBAQ 2902 GB-SD M M SEND TO PITTS CA GB-SD M M??? 7 HOLD GATLINBAQ 2905 GB-SD F F HOLD GATLINBAQ 2979 GB-SD F F HOLD GATLINBAQ 2984 GB-SD M M HOLD GATLINBAQ GB-SD M M HOLD GATLINBAQ GB-SD F F HOLD GATLINBAQ 2993 Double clutch; Repair 2987 GB-SD F F HOLD GATLINBAQ 2988 Double clutch 2988 GB-SD M M HOLD GATLINBAQ GB-SD F F HOLD GATLINBAQ 2984 Double clutch 2990 GB-SD F F HOLD GATLINBAQ 2985 Double clutch 2991 GB-SD M M HOLD GATLINBAQ 2992 Double clutch 2992 GB-SD F F HOLD GATLINBAQ 2991 Double clutch 2993 GB-SD M M HOLD GATLINBAQ GB-SD M M SEND TO ERIE GB-SD M M??? 6 HOLD GATLINBAQ 3017 GB-SD M M??? 5 HOLD GATLINBAQ 3018 GB-SD F F HOLD GATLINBAQ 3294 Double clutch; Repair 3124 GB-SD F F SEND TO ERIE 2994 Demographic pair 3125 GB-SD F F SEND TO PITTS CA 2902 Demographic pair 3211 GB-SD M M HOLD GATLINBAQ Available pending sire verification 3294 GG-SD M M HOLD GATLINBAQ 3018 Current holdings: 31 ( ); Holdings following transfers: 27 ( ) 59

61 Males > Draft for Review by Institutional Representatives Please comment by 21 Oct 2016 GATLINBAQ 2016 Females > ,2,3. 4s OK too. 5,6,"-" GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ 1629 GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GREEN NSC Natural Science Center of Greensboro 4301 Lawndale Drive, Greensboro, NC 27455, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD GREEN NSC M HOLD GREEN NSC F HOLD GREEN NSC M HOLD GREEN NSC F HOLD GREEN NSC 3194 Double clutch F SEND TO ADVENTURE Paired with sibling F HOLD GREEN NSC F HOLD GREEN NSC 2997 Double clutch F HOLD GREEN NSC M HOLD GREEN NSC M HOLD GREEN NSC F HOLD GREEN NSC F HOLD GREEN NSC M SEND TO ADVENTURE M RECEIVE FROM CINCINNAT M HOLD GREEN NSC F HOLD GREEN NSC M SEND TO CINCINNAT F HOLD GREEN NSC F HOLD GREEN NSC M HOLD GREEN NSC Paired with sibling 3079 Current holdings: 20 (9.11.0); Holdings following transfers: 18 (8.10.0) 60

62 GREENBAY NEW Zoo P.O. Box 23600, Green Bay, WI , USA Institution Notes: If space is limited, prioritize breeding pairs 2415 x 2971 and 2381 x ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD GREENBAY M HOLD GREENBAY F HOLD GREENBAY M HOLD GREENBAY 1854 Double clutch but low likelihood of success expected due to age Demographic pair - lower likelihood of success expected due to male's health Can breed with 3055 as an alternative 2381 M0187 M RECEIVE FROM RACINE M0306 M RECEIVE FROM RACINE M SEND TO RACINE F HOLD GREENBAY 2415 Double clutch F HOLD GREENBAY 3110 Demographic pair - may be a low likelihood of success due to female's history of imprinting M SEND TO RACINE F HOLD GREENBAY NOTES Alternate breeding female if needed for male M RECEIVE FROM LITTLEROC F HOLD GREENBAY 2381 Double clutch Current holdings: 10 (4.6.0); Holdings following transfers: 11 (5.6.0) 61

63 JENKINSON Jenkinson's Aquarium 300 Ocean Avenue, Point Pleasant Beach, NJ 08742, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 30 HOLD JENKINSON Age F??? 30 HOLD JENKINSON NOTES Age; Keep any fertile eggs produced by 1378 x M HOLD JENKINSON NOTES Paired with 842; keep fertile eggs from this pair M HOLD JENKINSON M HOLD JENKINSON M HOLD JENKINSON F HOLD JENKINSON F HOLD JENKINSON 1397 Double clutch M HOLD JENKINSON F??? 20 HOLD JENKINSON Medical F HOLD JENKINSON 2539 JA4751 F HOLD JENKINSON 2595 JA5031 M SEND TO STEINHART JA5836 M HOLD JENKINSON JA6449 F HOLD JENKINSON 3102 Double clutch 3102 JA6450 M HOLD JENKINSON JA6307 F HOLD JENKINSON 2765 Double clutch 3293 JA6398 M HOLD JENKINSON 3314 JA6434 M HOLD JENKINSON 3315 JA6435 F HOLD JENKINSON 3433 JA 656 M HOLD JENKINSON 3434 JA 657 M HOLD JENKINSON Current holdings: 22 ( ); Holdings following transfers: 21 ( ) AVAILABLE for indoor exhibits AVAILABLE for indoor exhibits KNOXVILLE Knoxville Zoological Gardens P.O. Box 6040, Knoxville, TN 37914, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 28 HOLD KNOXVILLE Age F??? 27 HOLD KNOXVILLE Age F HOLD KNOXVILLE M??? 23 HOLD KNOXVILLE M SEND TO ST PAUL F SEND TO ST PAUL M HOLD KNOXVILLE Current holdings: 7 (3.4.0); Holdings following transfers: 2 (2.3.0) Potential 62

64 LITTLEROC Little Rock Zoological Gardens One Jonesboro Dr, Little Rock, AR 72205, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 24 HOLD LITTLEROC Potential M HOLD LITTLEROC M??? 9 HOLD LITTLEROC Potential M HOLD LITTLEROC 2931 demog M HOLD LITTLEROC F HOLD LITTLEROC 2372 Demographic pair M NOTES NOTES NOTES AVAILABLE - SSP is looking for placement M HOLD LITTLEROC M SEND TO TULSA M NOTES NOTES NOTES AVAILABLE - SSP is looking for placement M HOLD LITTLEROC F HOLD LITTLEROC 2660 Demographic pair M HOLD LITTLEROC M SEND TO GREENBAY NOTES F HOLD LITTLEROC 2893 Demographic pair F HOLD LITTLEROC M??? 1 HOLD LITTLEROC F HOLD LITTLEROC M HOLD LITTLEROC Current holdings: 19 (13.6.0); Holdings following transfers: 17 (11.6.0) Potential LOWRY Tampa's Lowry Park Zoo 1101 W Sligh Ave, Tampa, FL , USA Institution Notes: If able to receive additional males, please contact the SSP Coordinator. ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 24 HOLD LOWRY Age/ Medical/ F??? 16 HOLD LOWRY Potential M HOLD LOWRY F HOLD LOWRY M RECEIVE FROM TAMPA AQ 2836 Pre-arranged transfer F HOLD LOWRY 2832 Double clutch M HOLD LOWRY F HOLD LOWRY 2837 Double clutch F HOLD LOWRY 2606 Double clutch M HOLD LOWRY F HOLD LOWRY 3388 UNK M HOLD LOWRY Can pair with F RECEIVE possible future TULSA FROM demographic breeding pair Current holdings: 11 (5.6.0); Holdings following transfers: 13 (6.7.0) 63

65 LVZOO Lehigh Valley Zoo 5150 Game Preserve Road, Schnecksville, PA , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes 2088 LV0239 M HOLD LVZOO LV0240 M HOLD LVZOO F RECEIVE FROM BALTIMORE 2497 LV0431 F HOLD LVZOO 2088 Demographic pair; 2089 is ok as an alternative male 2552 LV0123 M HOLD LVZOO 2612 LV0122 M HOLD LVZOO 2631 LV0119 M HOLD LVZOO 2639 LV0120 M HOLD LVZOO LV0121 F HOLD LVZOO 2733 Demographic pair 2700 LV0156 F HOLD LVZOO 2733 LV0117 M HOLD LVZOO LV0159 F HOLD LVZOO 2639 Demographic pair F??? F RECEIVE FROM RECEIVE FROM PITTS CA ROCHESTER Current holdings: 11 (7.4.0); Holdings following transfers: 14 (7.7.0) MADISON Henry Vilas Zoo 702 S Randall Ave, Madison, WI 53715, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 34 HOLD MADISON Age F??? 34 HOLD MADISON Age F??? 33 HOLD MADISON Age M??? 33 HOLD MADISON Age F??? 30 HOLD MADISON Age M??? 30 HOLD MADISON Age/ Unknown pedigree F??? 29 HOLD MADISON Age F HOLD MADISON M??? 8 HOLD MADISON 2959 B10007 M B10013 M B10015 F B10017 F RECEIVE FROM RECEIVE FROM RECEIVE FROM RECEIVE FROM GA AQUAR 2972 GA AQUAR 2970 GA AQUAR 2966 GA AQUAR F??? 3 HOLD MADISON F??? 1 HOLD MADISON Current holdings: 11 (4.7.0); Holdings following transfers: 15 (6.9.0) Potential Double clutch; Repair Double clutch; established pair Potential Potential 64

66 MEMPHIS Memphis Zoological Garden & Aquarium 2000 Prentiss Place, Memphis, TN 38112, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD MEMPHIS G548 F RECEIVE FROM DALLAS 2388 Double clutch F HOLD MEMPHIS M HOLD MEMPHIS F HOLD MEMPHIS M HOLD MEMPHIS M HOLD MEMPHIS F SEND TO CHICAGOLP M SEND TO CHICAGOLP 2742 SD0802 M RECEIVE FROM MYSTIC M HOLD MEMPHIS F HOLD MEMPHIS F HOLD MEMPHIS 2784 Demographic pair M HOLD MEMPHIS M HOLD MEMPHIS F HOLD MEMPHIS 2742 Double clutch F HOLD MEMPHIS 3008 Demographic pair F??? 3 HOLD MEMPHIS F??? 3 HOLD MEMPHIS A124 M HOLD MEMPHIS A078 M??? 2 HOLD MEMPHIS A080 F HOLD MEMPHIS A118 M HOLD MEMPHIS A119 M HOLD MEMPHIS A120 M HOLD MEMPHIS Current holdings: 23 ( ); Holdings following transfers: 23 ( ) 65

67 MINNESOTA Minnesota Zoological Garden Zoo Blvd., Apple Valley, MN , USA Institution Notes: If space allows, use the MateRx on the next page to identify additional breeding pairs to those that have already been recommended. MSIs of 1, 2, or 3 should be prioritized and may double clutch, while pairs with MSIs of 5, 6, or - are not recommended. Some MSIs of 4 may be necessary if better ranked pairs are unavailable or unsuccessful. Contact the SSP coordinator first for approval to breed any potential pairs using the MateRx matrix. ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD MINNESOTA Genetically valuable older female - priority to breed F HOLD MINNESOTA 1735 Double clutch M HOLD MINNESOTA F??? 22 HOLD MINNESOTA M HOLD MINNESOTA Potential F HOLD MINNESOTA 2980 Demographic pair F HOLD MINNESOTA 3328 Double clutch F HOLD MINNESOTA F HOLD MINNESOTA 3165 Demographic pair M HOLD MINNESOTA M??? 7 HOLD MINNESOTA F HOLD MINNESOTA M HOLD MINNESOTA F??? 6 HOLD MINNESOTA F??? 6 HOLD MINNESOTA F??? 6 HOLD MINNESOTA M HOLD MINNESOTA F HOLD MINNESOTA M HOLD MINNESOTA M HOLD MINNESOTA M HOLD MINNESOTA F??? 2 HOLD MINNESOTA M??? 2 HOLD MINNESOTA F HOLD MINNESOTA F??? 2 HOLD MINNESOTA M??? 2 HOLD MINNESOTA F??? 2 HOLD MINNESOTA F HOLD MINNESOTA Potential Potential Potential Potential Potential Potential Potential Potential Potential F HOLD MINNESOTA M RECEIVE FROM BUSCH TAM 2533 Double clutch F??? 0 HOLD MINNESOTA Potential M??? 0 HOLD MINNESOTA Potential 66

68 Males > Draft for Review by Institutional Representatives Please comment by 21 Oct 2016 ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 0 HOLD MINNESOTA M??? 0 HOLD MINNESOTA F??? 0 HOLD MINNESOTA F HOLD MINNESOTA F HOLD MINNESOTA M??? 0 HOLD MINNESOTA Current holdings: 37 ( ); Holdings following transfers: 38 ( ) Potential Potential Potential Potential MINNESOTA 2016 MateRx Females > ,2,3. 4s OK too. 5,6,"-" MINNESOTA MINNESOTA MINNESOTA MINNESOTA 3328 BUSCH TAM MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA MINNESOTA 67

69 MINOT Roosevelt Park Zoo 1219 Burdick Expressway East, Minot, ND 58702, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes 649 A35 M??? 33 HOLD MINOT Age 1466 A132 M HOLD MINOT 1485 A44 M HOLD MINOT 2135 A299 M SEND TO PUEBLO A300 M HOLD MINOT 2777 A397 M??? 7 HOLD MINOT 3278 A398 M HOLD MINOT 3283 A399 M HOLD MINOT M RECEIVE FROM ROCHESTER M RECEIVE FROM ROCHESTER M RECEIVE FROM ROCHESTER Current holdings: 8 (8.0.0); Holdings following transfers: 10 (10.0.0) MONTERYAQ Monterey Bay Aquarium 886 Cannery Row, Monterey, CA , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD MONTERYAQ 1610 ABFP12 F HOLD MONTERYAQ M HOLD MONTERYAQ F HOLD MONTERYAQ 2198 Double clutch F HOLD MONTERYAQ M HOLD MONTERYAQ M HOLD MONTERYAQ M HOLD MONTERYAQ M HOLD MONTERYAQ F??? 17 HOLD MONTERYAQ F HOLD MONTERYAQ M HOLD MONTERYAQ F HOLD MONTERYAQ F HOLD MONTERYAQ F HOLD MONTERYAQ F HOLD MONTERYAQ M??? 1 HOLD MONTERYAQ M??? 0 HOLD MONTERYAQ Current holdings: 18 (9.9.0); Holdings following transfers: 18 (9.9.0) Demographic pair Demographic pair 68

70 MYSTIC Mystic Aquarium 55 Coogan Blvd., Mystic, CT 06517, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD MYSTIC M HOLD MYSTIC F??? 28 HOLD MYSTIC Age F??? 28 HOLD MYSTIC Age M??? 28 HOLD MYSTIC Potential F??? 28 HOLD MYSTIC 1384 SD9101 M HOLD MYSTIC 1677 SD9208 F HOLD MYSTIC 1703 SD9302 F HOLD MYSTIC 1823 SD9402 M??? 22 HOLD MYSTIC Age/ Potential 2041 SD33 F HOLD MYSTIC 2047 SD9701 M??? 19 HOLD MYSTIC Potential 2107 SD9801 M??? 18 HOLD MYSTIC Potential 2108 SD9802 M??? 18 HOLD MYSTIC Potential 2291 SD36 M HOLD MYSTIC SD0201 F HOLD MYSTIC 2291 Double clutch 2558 SD0503 M SEND TO FT WAYNE SD0602 F??? 10 HOLD MYSTIC Potential 2575 SD0604 M HOLD MYSTIC 2621 SD0701 F??? 9 HOLD MYSTIC Medical/ 2742 SD0802 M SEND TO MEMPHIS SD0902 M??? 7 HOLD MYSTIC Potential 2936 SD1001 F??? 6 HOLD MYSTIC Potential 2937 SD1002 F HOLD MYSTIC double clutch 3030 SD1103 M??? 5 HOLD MYSTIC Potential 3178 SD1301 F??? 3 HOLD MYSTIC Potential 3180 SD1303 F??? 3 HOLD MYSTIC Potential 3297 SD1401 F??? 2 HOLD MYSTIC Potential 3298 SD1402 M HOLD MYSTIC 3299 SD1403 F??? 2 HOLD MYSTIC 3519 SD1504 F??? 0 HOLD MYSTIC 3520 SD1505 M??? 0 HOLD MYSTIC 3521 SD1601 F??? 0 HOLD MYSTIC 3522 SD1602 M HOLD MYSTIC Current holdings: 34 ( ); Holdings following transfers: 32 ( ) Potential Potential Potential Potential 69

71 NEWPORTKY Newport Aquarium One Aquarium Way, Newport, KY 41071, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD NEWPORTKY Potential F??? 10 HOLD NEWPORTKY Potential F??? 10 HOLD NEWPORTKY F HOLD NEWPORTKY F HOLD NEWPORTKY F HOLD NEWPORTKY F HOLD NEWPORTKY F??? 7 HOLD NEWPORTKY Current holdings: 8 (0.8.0); Holdings following transfers: 8 (0.8.0) Potential OMAHA Omaha's Henry Doorly Zoo 3701 South 10th St., Omaha, NE , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 39 HOLD OMAHA Age F??? 26 HOLD OMAHA Age M HOLD OMAHA M HOLD OMAHA F HOLD OMAHA M HOLD OMAHA M HOLD OMAHA F HOLD OMAHA F HOLD OMAHA M HOLD OMAHA F HOLD OMAHA M??? 17 HOLD OMAHA Unknown pedigree M??? 17 HOLD OMAHA Unknown F??? 15 HOLD OMAHA M HOLD OMAHA M HOLD OMAHA M HOLD OMAHA M HOLD OMAHA M HOLD OMAHA F??? 7 HOLD OMAHA Current holdings: 20 (12.8.0); Holdings following transfers: 20 (12.8.0) pedigree Potential Potential 70

72 PITTS CA National Aviary in Pittsburgh Allegheny Commons West, Pittsburgh, PA 15212, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 14 HOLD PITTS CA Potential F HOLD PITTS CA 2833 Double clutch; Repair M HOLD PITTS CA M??? 12 HOLD PITTS CA Potential F??? 7 HOLD PITTS CA Potential M HOLD PITTS CA F HOLD PITTS CA F HOLD PITTS CA M HOLD PITTS CA 2446 GB-SD- RECEIVE 2902 M GATLINBAQ M FROM Demographic pair; Repair F??? 5 SEND TO LVZOO Potential F??? 4 HOLD PITTS CA Potential M??? 4 HOLD PITTS CA Potential 3125 GB-SD- RECEIVE F F FROM GATLINBAQ 2902 Demographic pair M HOLD PITTS CA F??? 2 HOLD PITTS CA Potential F??? 2 HOLD PITTS CA Potential M??? 2 HOLD PITTS CA Potential F??? 1 HOLD PITTS CA Potential M??? 1 HOLD PITTS CA Potential Current holdings: 18 (9.9.0); Holdings following transfers: 19 (10.9.0) 71

73 PUEBLO Pueblo Zoo 3455 Nuckolls Ave., Pueblo, CO 81005, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 35 HOLD PUEBLO Age F HOLD PUEBLO F??? 25 HOLD PUEBLO Age 1784 B32011 M??? 22 SEND TO DALLAS Potential M HOLD PUEBLO 2022 B24001 F HOLD PUEBLO 2135 Double clutch 2135 A299 M RECEIVE FROM MINOT B25023 M SEND TO CHICAGOLP 2579 Pre-arranged transfer 2638 B27005 F SEND TO CHICAGOLP Pre-arranged transfer 2894 B29021 F HOLD PUEBLO 2998 B30028 F HOLD PUEBLO 3510 Double clutch; 3513 ok as alternative 3136 B31020 F HOLD PUEBLO 3513 Double clutch; 3510 ok as alternative 3432 B36001 M HOLD PUEBLO T009 M RECEIVE FROM DALLAS T011 M RECEIVE FROM DALLAS 3136 Current holdings: 12 (5.7.0); Holdings following transfers: 12 (6.6.0) RACINE Racine Zoological Gardens 2131 N Main St., Racine, WI , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 31 HOLD RACINE Age 1413 M0716 F??? 25 HOLD RACINE Age/ Medical/ Imprinted 1903 M1525 M HOLD RACINE 2381 M0187 M SEND TO GREENBAY M0306 M SEND TO GREENBAY M1524 M HOLD RACINE 2578 M1203 F HOLD RACINE 3177 Demographic pair 2699 M1523 M HOLD RACINE 2906 M1526 M??? 7 HOLD RACINE 3177 M1427 M HOLD RACINE M1601 F HOLD RACINE 3504 M1602 M HOLD RACINE M RECEIVE FROM GREENBAY M RECEIVE FROM GREENBAY Current holdings: 12 (8.4.0); Holdings following transfers: 12 (8.4.0) 72

74 ROCHESTER Seneca Park Zoo 2222 St Paul St., Rochester, NY , USA Institution Notes: If space allows, use the MateRx on the next page to identify additional breeding pairs to those that have already been recommended. MSIs of 1, 2, or 3 should be prioritized and may double clutch, while pairs with MSIs of 5, 6, or - are not recommended. Some MSIs of 4 may be necessary if better ranked pairs are unavailable or unsuccessful. Contact the SSP coordinator first for approval to breed any potential pairs using the MateRx matrix. ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 21 HOLD ROCHESTER Potential M HOLD ROCHESTER M HOLD ROCHESTER M HOLD ROCHESTER M HOLD ROCHESTER M HOLD ROCHESTER F HOLD ROCHESTER 2203 Double clutch M HOLD ROCHESTER M HOLD ROCHESTER F HOLD ROCHESTER F HOLD ROCHESTER F HOLD ROCHESTER 2290 Demographic pair M HOLD ROCHESTER F HOLD ROCHESTER 2061 Demographic pair F HOLD ROCHESTER Potential M??? 8 HOLD ROCHESTER M HOLD ROCHESTER F HOLD ROCHESTER F HOLD ROCHESTER 2822 Demographic pair M HOLD ROCHESTER F HOLD ROCHESTER F HOLD ROCHESTER M SEND TO MINOT M SEND TO MINOT M SEND TO MINOT F HOLD ROCHESTER F SEND TO ADVENTURE M SEND TO ADVENTURE F SEND TO LVZOO M SEND TO ADVENTURE U HOLD ROCHESTER U HOLD ROCHESTER Current holdings: 32 ( ); Holdings following transfers: 25 ( ) 73

75 Males > Draft for Review by Institutional Representatives Please comment by 21 Oct 2016 ROCHESTER 2016 MateRx Females > ,2,3. 4s OK too. 5,6,"-" ROCHESTER ROCHESTER ROCHESTER ROCHESTER 1962 ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER ROCHESTER SAGINAW Children's Zoo at Celebration Square Saginaw Zool. Society, Saginaw, MI , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD SAGINAW M RECEIVE FROM BUSCH TAM M HOLD SAGINAW F HOLD SAGINAW F SEND TO BUSCH TAM 3529 Double clutch F HOLD SAGINAW 3530 Double clutch M HOLD SAGINAW M HOLD SAGINAW Current holdings: 7 (3.4.0); Holdings following transfers: 7 (4.3.0) 74

76 SANDIEGOZ San Diego Zoo PO Box , San Diego, CA , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD SANDIEGOZ F HOLD SANDIEGOZ M HOLD SANDIEGOZ M HOLD SANDIEGOZ F HOLD SANDIEGOZ M HOLD SANDIEGOZ F??? M RECEIVE FROM RECEIVE FROM Current holdings: 6 (4.2.0); Holdings following transfers: 8 (5.3.0) TOLEDO TOLEDO Potential ; Pre-arranged transfer for fall 2016 Pre-arranged transfer for fall 2016 SPRINGFIE Henson Robinson Zoo 1100 E Lake Dr., Springfield, IL 62712, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD SPRINGFIE F HOLD SPRINGFIE F HOLD SPRINGFIE M HOLD SPRINGFIE M HOLD SPRINGFIE Current holdings: 5 (2.3.0); Holdings following transfers: 5 (2.3.0) 75

77 ST PAUL St. Paul's Como Zoo 1225 Estabrook Drive, St Paul, MN 55103, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 35 HOLD ST PAUL Age M??? 35 HOLD ST PAUL Age M HOLD ST PAUL F HOLD ST PAUL M HOLD ST PAUL M RECEIVE FROM KNOXVILLE M HOLD ST PAUL F HOLD ST PAUL M HOLD ST PAUL F RECEIVE FROM KNOXVILLE M HOLD ST PAUL F HOLD ST PAUL Current holdings: 10 (6.4.0); Holdings following transfers: 12 (7.5.0) STEINHART Steinhart Aquarium CA Acad. of Science 55 Music Concourse Drive, San Francisco, CA 94118, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes F??? 32 HOLD STEINHART Age F??? 28 HOLD STEINHART Age M HOLD STEINHART F HOLD STEINHART 2595 Demographic pair but low likelihood of success expected due to age M HOLD STEINHART 2595 JA5031 M RECEIVE FROM JENKINSON M HOLD STEINHART F HOLD STEINHART F??? 9 HOLD STEINHART M HOLD STEINHART F HOLD STEINHART 3173 Demographic pair F HOLD STEINHART 2375 Demographic pair M HOLD STEINHART M HOLD STEINHART M HOLD STEINHART M HOLD STEINHART M HOLD STEINHART Current holdings: 16 (9.7.0); Holdings following transfers: 17 (10.7.0) 76

78 TAMPA AQ Florida Aquarium 701 Channelside Drive, Tampa, FL 33602, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD TAMPA AQ M SEND TO LOWRY 2836 Pre-arranged transfer M HOLD TAMPA AQ M HOLD TAMPA AQ M HOLD TAMPA AQ F HOLD TAMPA AQ M HOLD TAMPA AQ F HOLD TAMPA AQ F HOLD TAMPA AQ F??? 3 HOLD TAMPA AQ Current holdings: 10 (6.4.0); Holdings following transfers: 9 (5.4.0) Potential TAUTPHAUS Tautphaus Park Zoo PO Box 50220, Idaho Falls, ID , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes 959 K0A029 M HOLD TAUTPHAUS A013 M HOLD TAUTPHAUS A016 M HOLD TAUTPHAUS A012 M HOLD TAUTPHAUS A001 F??? 21 HOLD TAUTPHAUS Potential A002 F??? 21 HOLD TAUTPHAUS Potential A004 F HOLD TAUTPHAUS 1770 Double clutch A016 F??? 17 HOLD TAUTPHAUS Potential 2586 K6A027 F HOLD TAUTPHAUS Valuable female 2702 K7A139 F HOLD TAUTPHAUS 959 Double clutch 3011 KXA022 M??? 6 HOLD TAUTPHAUS Potential 3012 KXA023 M??? 6 HOLD TAUTPHAUS Potential 3014 KXA025 F HOLD TAUTPHAUS 3237 Double clutch; Repair A033 M HOLD TAUTPHAUS A050 M HOLD TAUTPHAUS A023 F??? 1 HOLD TAUTPHAUS A017 U HOLD TAUTPHAUS Current holdings: 17 (8.8.1); Holdings following transfers: 17 (8.8.1) Potential 77

79 TOLEDO Toledo Zoological Gardens PO Box , Toledo, OH , USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD TOLEDO M HOLD TOLEDO M HOLD TOLEDO M??? 16 HOLD TOLEDO Potential M HOLD TOLEDO M??? 15 HOLD TOLEDO Potential M??? 15 HOLD TOLEDO M HOLD TOLEDO F HOLD TOLEDO F??? 7 HOLD TOLEDO Potential Potential M HOLD TOLEDO M HOLD TOLEDO M HOLD TOLEDO F HOLD TOLEDO 2418 Double clutch F HOLD TOLEDO 2935 Demographic pair F HOLD TOLEDO 2934 Double clutch F HOLD TOLEDO M HOLD TOLEDO F HOLD TOLEDO Genetically valuable F??? 0 SEND TO SANDIEGOZ M SEND TO SANDIEGOZ F??? 0 HOLD TOLEDO Current holdings: 22 (13.9.0); Holdings following transfers: 20 (12.8.0) Potential ; Pre-arranged transfer for fall 2016 Pre-arranged transfer for fall 2016 Potential 78

80 TORONTO Toronto Zoo 361A Old Finch Ave., Scarborough, Ontario M1B 5K7, Canada ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 26 HOLD TORONTO M HOLD TORONTO F??? 20 HOLD TORONTO M??? 19 HOLD TORONTO M HOLD TORONTO F HOLD TORONTO F HOLD TORONTO 3151 Double clutch; Repair M HOLD TORONTO F HOLD TORONTO M HOLD TORONTO F HOLD TORONTO 3152 Double clutch M HOLD TORONTO M HOLD TORONTO M HOLD TORONTO M HOLD TORONTO M HOLD TORONTO M HOLD TORONTO F??? 3 HOLD TORONTO M??? 1 HOLD TORONTO M HOLD TORONTO F HOLD TORONTO F??? 1 HOLD TORONTO M??? 1 HOLD TORONTO Current holdings: 23 (15.8.0); Holdings following transfers: 23 (15.8.0) 79

81 TULSA Tulsa Zoo 5701 E 36th St N, Tulsa, OK 74115, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M??? 33 HOLD TULSA Age F??? 27 HOLD TULSA Age/ F HOLD TULSA F HOLD TULSA 2885 Demographic pair F HOLD TULSA M HOLD TULSA M HOLD TULSA F HOLD TULSA M HOLD TULSA M HOLD TULSA M??? 16 HOLD TULSA M HOLD TULSA M HOLD TULSA F HOLD TULSA F??? 13 HOLD TULSA F??? 13 HOLD TULSA F??? 13 HOLD TULSA M RECEIVE FROM LITTLEROC 1650 Potential Potential Potential Potential M??? 5 HOLD TULSA Potential F HOLD TULSA 2138 Double clutch; Repair M??? 1 HOLD TULSA Potential M HOLD TULSA F SEND TO LOWRY Pair with M??? 0 HOLD TULSA Potential F??? 0 HOLD TULSA Potential Current holdings: 24 ( ); Holdings following transfers: 24 ( ) 80

82 VANCOUVAQ Vancouver Aquarium Marine Science Ctr P.O. Box 3232, Vancouver, BC V6B 3X8, Canada ID Local ID Sex MK Age Disposition Location Breeding With Notes F HOLD VANCOUVAQ F HOLD VANCOUVAQ M HOLD VANCOUVAQ F HOLD VANCOUVAQ F HOLD VANCOUVAQ M HOLD VANCOUVAQ F HOLD VANCOUVAQ Current holdings: 7 (2.5.0); Holdings following transfers: 7 (2.5.0) W ORANGE Turtle Back Zoo 560 Northfield Ave., West Orange, NJ 07052, USA ID Local ID Sex MK Age Disposition Location Breeding With Notes M HOLD W ORANGE M HOLD W ORANGE F HOLD W ORANGE F HOLD W ORANGE 2691 Demographic pair M HOLD W ORANGE F HOLD W ORANGE M HOLD W ORANGE M HOLD W ORANGE F HOLD W ORANGE 2910 Double clutch M HOLD W ORANGE M HOLD W ORANGE M RECEIVE FROM BOS NEAQ 3245 Current holdings: 11 (7.4.0); Holdings following transfers: 12 (8.4.0) 81

83 Analytical Data for True Individuals Appendix A Analytical Assumptions Studbook ID Field True Studbook Analytical Overlay Notes Dam UNK 48 Sire UNK 45 Detroit imported 7 wild-caught birds in Parents for hatches from (and a few subsequently) were not recorded. Wild caught Dam UNK 48 birds and some progeny were later sent to Los Angeles, and Sire Dam UNK UNK descendants were disbursed from there. Because the Detroit birds represent valuable unique lineages, it was decided to arbitrarily assign Sire UNK 43 the birds coming from Detroit a progeny of two pairs of the original wild-caught birds. Those arbitrary assignments were: Progeny Dam UNK 44 assigned to 45 x 48: 152, 188; Progeny assigned to 43 x 44: 99, 222. Sire UNK 43 Dam UNK STEIN2 MarineLand of the Pacific (now defunct) sent four birds (526, 580, 603, Sire UNK STEIN1 633) to Steinhart that became ancestors of many birds in the current population. The MarineLand population contained several Spheniscus Dam UNK STEIN2 species and it is possible that some of the birds identified as African Sire UNK STEIN1 penguins may have been s. Descendants of these birds were not Dam UNK STEIN2 used in prior breeding plans. However, genetic testing has recently indicated that the four birds are pure African penguins. It is not known Sire UNK STEIN1 that the relationships were among the birds that came from MarineLand of the Pacific, but it is likely that they were unrelated to Dam UNK STEIN2 penguins in other colonies. For the analyses, we made the assumption that the four birds were full siblings, with hypothetical wild-caught parents (STEIN1 x STEIN2). This assumption has now allowed many Sire UNK STEIN1 birds to be used in the breeding program that had been excluded in prior years. Five birds of unknown parentage (204, 205, 279, 280, and 281) first arrived in zoos in the 1970s at MICH KEN (Ken Chisholm, Montreal). Dam UNK MIKE2 Although the parents are unknown, it is most likely that they were founders (either wild caught or from Europe); however, it is possible that these 5 birds in the studbook are related in some way. For the purposes of the analyses, the birds from MICH KEN were treated as Sire UNK MIKE1 though they were full siblings produced by the mating of two hypothetical wild-caught founders (MIKE1 x MIKE2). However, all of these birds except #280, are now dead with no living descendants. Dam UNK WILD1 The black hole equation was used to determine that animals exported Sire UNK HYP1 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD2 The black hole equation was used to determine that animals exported Sire UNK HYP2 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD3 The black hole equation was used to determine that animals exported Sire UNK HYP3 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD4 The black hole equation was used to determine that animals exported Sire UNK HYP4 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD5 The black hole equation was used to determine that animals exported Sire UNK HYP5 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD6 The black hole equation was used to determine that animals exported Sire UNK HYP6 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD7 The black hole equation was used to determine that animals exported Sire UNK HYP7 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD8 The black hole equation was used to determine that animals exported Sire UNK HYP8 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD9 Sire UNK HYP9 The black hole equation was used to determine that animals exported from AMSTERDAM are equivalent to first cousins. 82

84 Studbook ID Field True Studbook Analytical Overlay Notes Dam UNK WILD10 The black hole equation was used to determine that animals exported Sire UNK HYP10 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD11 The black hole equation was used to determine that animals exported Sire UNK HYP11 from AMSTERDAM are equivalent to first cousins Dam UNK WILD12 The black hole equation was used to determine that animals exported Sire UNK HYP12 from AMSTERDAM are equivalent to first cousins Sire UNK HYP14 Given hypothetical sire HYP13 (from HYP14 x WILD14 parents); created to make #2866 from the AMSTERDAM lineage, but another generation removed since he hatched almost 20 years after these other AMSTERDAM birds Dam UNK WILD15 The black hole equation was used to determine that animals exported Sire UNK HYP15 from AMSTERDAM are equivalent to first cousins Dam UNK HYP Hypothetical Individuals Studbook ID The black hole equation was used to determine that animals exported from AMSTERDAM are equivalent to first cousins. Dam UNK WILD17 The black hole equation was used to determine that animals exported Sire UNK HYP17 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD18 The black hole equation was used to determine that animals exported Sire UNK HYP18 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD19 The black hole equation was used to determine that animals exported Sire UNK HYP19 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD20 The black hole equation was used to determine that animals exported Sire UNK HYP20 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD21 The black hole equation was used to determine that animals exported Sire UNK HYP21 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD22 The black hole equation was used to determine that animals exported Sire UNK HYP22 from AMSTERDAM are equivalent to first cousins. Dam UNK WILD23 The black hole equation was used to determine that animals exported Sire UNK HYP23 from AMSTERDAM are equivalent to first cousins. Sire Dam Notes HYP1 HYP24 HYP25 HYP10 HYP24 HYP25 HYP11 HYP24 HYP25 HYP12 HYP24 HYP25 HYP13 HYP24 HYP25 HYP14 HYP13 WILD13 HYP15 HYP24 HYP25 HYP16 HYP24 HYP25 HYP17 HYP24 HYP25 Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP13 x WILD13; created to make #2866 from the AMSTERDAM lineage, but another generation removed since he hatched almost 20 years after these other AMSTERDAM birds. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. 83

85 Studbook ID Sire Dam Notes HYP18 HYP24 HYP25 HYP19 HYP24 HYP25 HYP2 HYP24 HYP25 HYP20 HYP24 HYP25 HYP21 HYP24 HYP25 HYP22 HYP24 HYP25 HYP23 HYP24 HYP25 HYP24 WILD WILD HYP25 WILD WILD HYP3 HYP24 HYP25 HYP4 HYP24 HYP25 HYP5 HYP24 HYP25 HYP6 HYP24 HYP25 HYP7 HYP24 HYP25 HYP8 HYP24 HYP25 Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical grandparents of all Amsterdam animals. Georgia Aquarium obtained penguins from Amsterdam in Baltimore (in 1992), Denver (in 1982), and Toronto (in 1972) had previously obtained some birds from Amsterdam. This Amsterdam population was started from 11 wild-caught founders and was maintained as a closed population for 23 years. The population had gone through several bottlenecks during disease outbreaks prior to shipment of birds to North America. No pedigrees of the birds imported from Amsterdam are available, and it is likely that they were related. In past SSP analyses, the birds imported from Amsterdam were treated as full siblings produced by the mating of two hypothetical wild caught founders. However, due to the recent import of more birds from AMSTERDAM, a black hole equation was used to determine the estimated relatedness of the living descendants in this population. This method indicates that the 23 animals that have been exported from AMSTERDAM are equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. HYP9 HYP24 HYP25 MIKE1 WILD WILD Parents of 280. MIKE2 WILD WILD Parents of 280. Hypothetical offspring of HYP24 x HYP25; created to make all imports from AMSTERDAM equivalent to first cousins. STEIN1 WILD WILD Parents of 526, 580, 603, and 633. STEIN2 WILD WILD Parents of 526, 580, 603, and

86 Appendix B Summary of Data Exports PMx Project: AfrPenguin2_18July2016 Created: by PMx version File: C:\PMxProjects\Penguin, African\2016 SSP Planning\18 July 2016\AfrPenguin2_18July2016.pmxproj Studbook Information: Common Name: AFRICAN PENGUIN Scientific Name: SPHENISCUS DEMERSUS Data Source: PopLink Studbook Name: AfricanPenguin_6july2016_13Jul16 Exported On: Software version: PopLink 2.4 Current through: Compiled by: Seana Flossic Scope: North American Regional User: ssullivan Primary genetic data file Data File Name: XX3044updated_13Jul16.ped Genetic Filters: Association = AZA.FED, Status = Living as of Locations data file Data File Name: location.txt Census1 file: Exchcens.txt (AZA.FED) Demographic input files MPrn file: mxxafricanpenguin_6july2016_13jul16.prn FPrn file: fxxafricanpenguin_6july2016_13jul16.prn Demographic filters: Association = AZA.FED, Status = Living during to Selected population was changed from the originally imported data. Deaths reported during the draft period: CHICAGOLP M 2594, KNOXVILLE M 1910, LITTLEROC M 2094, MYSTIC M 1387 Citations: Bos, C., Schad, K, and Fienieg, E Long-term management plan for the African Penguin (Spheniscus demersus) European Endangered Species Programme (EEP). Artis Royal Zoo, Amsterdam, Netherlands. 85

87 Appendix C Individuals Excluded from the Genetic Analysis ID Location Age Sex Notes 298 OMAHA 39 F Age 429 ST PAUL 36 F Age 461 ST PAUL 35 M Age 487 PUEBLO 35 M Age 500 BOS NEAQ 35 F Age 519 MADISON 34 M Age 526 AQUARAMER 35 M Age 527 MADISON 34 F Age 588 TULSA 34 M Age 604 MADISON 33 F Age 624 MADISON 33 M Age 649 MINOT 33 M Age 660 DENVER 33 M Age 683 CINCINNAT 32 M Age/ Unknown pedigree 715 STEINHART 32 F Age 716 BOS NEAQ 32 F Age 727 FORTWORTH 32 F Age 747 RACINE 32 F Age 833 MADISON 30 F Age 839 JENKINSON 31 F Age 840 GA AQUAR 31 M Age 842 JENKINSON 30 F Age 852 MADISON 30 M Age/ Unknown pedigree 872 MADISON 30 F Age 878 BALTIMORE 30 F Age 937 GA AQUAR 29 F Age 974 MYSTIC 29 F Age 987 MYSTIC 29 F Age 990 MYSTIC 29 M - Descendant of KNOXVILLE 29 F Age 1019 MYSTIC 29 F Age/ - descendant of STEINHART 28 F Age 1111 AQUARAMER 27 M Age/ Medical 1147 GA AQUAR 27 F Age 1166 TULSA 27 F Age/ - descendant of KNOXVILLE 27 F Age 1203 OMAHA 27 F Age 1240 AQUARAMER 27 F Age 1270 TORONTO 26 M - Descendant of FORTWORTH 26 M - Descendant of AQUARAMER 26 F Age/ Medical 1393 PUEBLO 25 F Age 1413 RACINE 25 F Age/Medical/Imprinted 1448 AQUARAMER 25 F Age/ Education 1473 LITTLEROC 25 F - Descendant of DENVER 25 F - Descendant of LOWRY 24 M Medical/ Age/ - descendant of KNOXVILLE 24 M - Descendent of MINNESOTA 23 F - Descendant of PUEBLO 23 M - Descendant of GA AQUAR 22 F - Descendant of MYSTIC 22 M - Descendant of ROCHESTER 22 F - Descendant of

88 ID Location Age Sex Notes 1870 TAUTPHAUS 22 F - Descendant of TAUTPHAUS 22 F - Descendant of JENKINSON 20 F Medical 1984 TORONTO 20 F - Descendant of BALTIMORE 20 F - Descendant of MYSTIC 19 M - Descendant of TORONTO 19 M - Descendant of MYSTIC 18 M - Descendant of MYSTIC 18 M - Descendant of ADVENTURE 19 M - Descendant of TAUTPHAUS 18 F - Descendant of OMAHA 17 M Unknown pedigree 2226 MONTERYAQ 17 F - Descendant of FORTWORTH 18 F - Descendant of OMAHA 17 M Unknown pedigree 2293 TOLEDO 16 M - Descendant of TULSA 16 M - Descendant of BALTIMORE 16 F - Descendant of TOLEDO 16 M - Descendant of TOLEDO 16 M - Descendant of OMAHA 15 F - Descendant of AQUARAMER 15 M - Descendant of PITTS CA 15 M - Descendant of 663 and ADVENTURE 15 M - Descendant of LOWRY 16 F - Descendant of TULSA 13 F - Descendant of DALLAS WA 12 M - Descendant of TULSA 13 F - Descendant of PITTS CA 13 M - Descendant of MYSTIC 10 F - Descendant of LITTLEROC 10 M - Descendant of MYSTIC 9 F Medical/ - descendant of BROOKLYN 9 M - Descendant of NEWPORTKY 10 F - Descendant of NEWPORTKY 10 F - Descendant of CALDWELL 9 F - Descendant of BROOKLYN 9 F - Descendant of 663 and STEINHART 9 F - Descendant of 663 and CHICAGOLP 8 M - Descendant of PITTS CA 8 F - Descendant of MADISON 8 M - Descendant of DENVER 8 M - Descendant of DENVER 8 F - Descendant of GATLINBAQ 9 M - Descendant of 658 and ROCHESTER 9 M - Descendant of MYSTIC 7 M - Descendant of OMAHA 7 F - Descendant of BALTIMORE 7 F Education/imprinted/ - descendant of MINNESOTA 8 M - Descendant of MINOT 8 M - Descendant of BALTIMORE 7 M - Descendant of TOLEDO 8 F - Descendant of GA AQUAR 15 F Suspected to be a 2874 TULSA 13 F Suspected to be a 2876 GA AQUAR 11 F Suspected to be a 2877 GA AQUAR 11 M Unknown pedigree/ 2878 GA AQUAR 11 M Suspected to be a 87

89 ID Location Age Sex Notes 2879 GA AQUAR 11 M Unknown pedigree/ 2881 GA AQUAR 11 M Suspected to be a 2890 ADVENTURE 7 M Medical 2899 GA AQUAR 7 M - Descendant of GATLINBAQ 7 M - Descendant of 663 and NEWPORTKY 7 F - Descendant of RACINE 7 M - Descendant of 663 and BALTIMORE 7 F - Descendant of MINNESOTA 7 F - Descendant of MINNESOTA 6 F - Descendant of MINNESOTA 6 F - Descendant of MYSTIC 6 F - Descendant of TULSA 6 M - Descendant of GA AQUAR 6 F Medical 2996 GATLINBAQ 7 M - Descendant of COLUMBUS 6 F - Descendant of COLUMBUS 6 F - Descendant of TAUTPHAUS 6 M - Descendant of TAUTPHAUS 6 M - Descendant of DALLAS 6 F - Descendant of 663 and GATLINBAQ 6 M - Descendant of PITTS CA 6 F - Descendant of DALLAS 6 M - Descendant of 663 and DENVER 6 M - Descendant of MYSTIC 5 M - Descendant of BALTIMORE 6 M - Descendant of BALTIMORE 5 M - descendant of PITTS CA 4 F - Descendant of PITTS CA 4 M - Descendant of BROOKLYN 4 F - Descendant of ADVENTURE 4 F - Descendant of MEMPHIS 4 F - Descendant of MEMPHIS 4 F - Descendant of MADISON 3 F - Descendant of MYSTIC 3 F - Descendent of MYSTIC 3 F - Descendant of BROOKLYN 5 F - Descendant of BOS NEAQ 5 M - Descendant of TAMPA AQ 3 F - Descendant of GA AQUAR 3 F - Descendant of BROOKLYN 3 M - Descendant of 663 and AQUARAMER 3 M - Descendant of AQUARAMER 3 F - Descendant of BALTIMORE 3 M - Descendant of BALTIMORE 3 M - Descendant of PITTS CA 3 F - Descendant of PITTS CA 3 F - Descendant of PITTS CA 3 M - Descendant of BALTIMORE 3 M - Descendant of MINNESOTA 3 F - Descendant of MINNESOTA 3 M - Descendant of MINNESOTA 3 F - Descendant of MINNESOTA 3 M - Descendant of MINNESOTA 3 F - Descendant of BROOKLYN 3 F - Descendant of 663 and MYSTIC 2 F - Descendant of MYSTIC 2 F - Descendant of

90 ID Location Age Sex Notes 3302 BALTIMORE 2 M - Descendant of BALTIMORE 6 F Education/imprinted/ - descendant of BALTIMORE 2 M - Descendant of COLUMBUS 2 M - Descendant of COLUMBUS 2 M - Descendant of GA AQUAR 2 M Medical/ - descendant of GA AQUAR 2 M Medical 3333 BROOKLYN 2 M - Descendant of 663 and BROOKLYN 2 M - Descendant of 663 and COLUMBUS 2 F - Descendant of MEMPHIS 3 M - Descendant of TAUTPHAUS 2 F - Descendant of TORONTO 3 F - Descendant of 658 and BUSCH TAM 2 M - Descendant of LITTLEROC 2 M - Descendant of 663 and BALTIMORE 2 F - Descendant of TULSA 2 M - Descendant of BROOKLYN 2 M - Descendant of 663 and PITTS CA 2 F - Descendant of PITTS CA 2 M - Descendant of BALTIMORE 2 M - Descendant of CHICAGOLP 2 M - Descendant of CHICAGOLP 2 F - Descendant of TORONTO 2 M - Descendant of TORONTO 2 F - Descendant of 658 and TORONTO 2 M - Descendant of 658 and BROOKLYN 1 M - Descendant of 663 and CHICAGOLP 1 M - Descendant of MADISON 1 F - Descendant of CHICAGOLP 1 M - Descendant of MONTERYAQ 1 M - Descendant of GA AQUAR 1 F - Descendant of GA AQUAR 1 M - Descendant of DENVER 1 M - Descendant of TULSA 1 M - Descendant of BROOKLYN 1 M - Descendant of TOLEDO 1 F - Descendant of 677 and MINNESOTA 1 F - Descendant of MINNESOTA 1 M - Descendant of MINNESOTA 1 M - Descendant of BALTIMORE 1 F - Descendant of TOLEDO 0 F - Descendant of MYSTIC 1 F - Descendant of MYSTIC 1 M - Descendant of MYSTIC 1 F - Descendant of MINNESOTA 0 M - Descendant of MINNESOTA 0 F - Descendant of TULSA 0 F - Descendant of BALTIMORE 0 M - Descendant of GA AQUAR 0 M - Descendant of BALTIMORE 0 M - Descendant of MINNESOTA 0 M - Descendant of MONTERYAQ 0 M - Descendant of DENVER 0 M - Descendant of

91 Appendix D Life Tables Qx = mortality; Px = survival; Lx = cumulative survivorship; Mx = fecundity; Vx = expected future reproduction Males Age (years) Px Qx Risk Qx Lx Mx Risk Mx Ex Vx r=0.050 λ=1.054 Ro=1.631 T=9.8 N20=926 90

92 Qx = mortality; Px = survival; Lx = cumulative survivorship; Mx = fecundity; Vx = expected future reproduction Females Age (years) Px Qx Risk Qx Lx Mx Risk Mx Ex Vx r=0.056 λ=1.057 Ro=1.584 T=8.3 N20=926 91

93 Appendix E Ordered Mean Kinship List This list is current to July 2016 and values are subject to change with any hatch, death, import, export, inclusion, or exclusion. Unknown sex birds appear on both the male and female sides of the MK list. Population Average MK = Male Female ID MK Known Age Location ID MK Known Age Location GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GATLINBAQ GA AQUAR GATLINBAQ GATLINBAQ GREENBAY GA AQUAR GA AQUAR GA AQUAR GREENBAY GATLINBAQ BUSCH TAM GA AQUAR GA AQUAR GATLINBAQ JENKINSON GATLINBAQ GATLINBAQ GA AQUAR BUSCH TAM GA AQUAR BUSCH TAM GA AQUAR GA AQUAR GA AQUAR LOWRY FORTWORTH FORTWORTH COLUMBUS COLUMBUS LOWRY GA AQUAR BUSCH TAM GA AQUAR BUSCH TAM GA AQUAR BUSCH TAM TOLEDO BUSCH TAM LOWRY MEMPHIS GA AQUAR PITTS CA GA AQUAR GA AQUAR GA AQUAR ADVENTURE LOWRY MEMPHIS GA AQUAR GA AQUAR KNOXVILLE GA AQUAR ST PAUL OMAHA PUEBLO ST PAUL PUEBLO BALTIMORE BALTIMORE RACINE TOLEDO ST PAUL W ORANGE TOLEDO FORTWORTH GREEN NSC SAGINAW JENKINSON SAGINAW TAUTPHAUS GREENBAY LOWRY DALLAS GA AQUAR ADVENTURE ADVENTURE FT WAYNE BOS NEAQ LOWRY FORTWORTH TULSA TAUTPHAUS DALLAS DALLAS LVZOO DALLAS SAGINAW RACINE TOLEDO BALTIMORE MEMPHIS MYSTIC GREEN NSC CINCINNAT BOS NEAQ MEMPHIS MYSTIC LVZOO MINNESOTA LVZOO MYSTIC MINNESOTA GATLINBAQ GATLINBAQ TORONTO TAUTPHAUS BROOKLYN 92

94 Male Female ID MK Known Age Location ID MK Known Age Location W ORANGE COLO SPRG COLO SPRG BOS NEAQ BOS NEAQ BALTIMORE MYSTIC TAUTPHAUS AQUARAMER TORONTO W ORANGE CINCINNAT MYSTIC ADVENTURE MYSTIC TAUTPHAUS CINCINNAT FORTWORTH MINOT GREENBAY TAUTPHAUS TULSA OMAHA FT WAYNE OMAHA MONTERYAQ JENKINSON BOS NEAQ JENKINSON JENKINSON AQUARAMER TORONTO LVZOO SANDIEGOZ TORONTO MEMPHIS BOS NEAQ MINNESOTA BOS NEAQ CINCINNAT BOS NEAQ JENKINSON BOS NEAQ BOS NEAQ ROCHESTER GA AQUAR MYSTIC STEINHART MYSTIC GREEN NSC TORONTO TULSA BOS NEAQ TOLEDO COLO SPRG ROCHESTER BALTIMORE MONTERYAQ FT WAYNE CALDWELL MINOT TAUTPHAUS TOLEDO MINNESOTA BALTIMORE BOS NEAQ TAMPA AQ BOS NEAQ TAMPA AQ DALLAS TAMPA AQ BOS NEAQ CINCINNAT LITTLEROC TULSA BOS NEAQ JENKINSON TULSA MONTERYAQ PUEBLO BALTIMORE BUSCH TAM LITTLEROC BOS NEAQ GA AQUAR MYSTIC GA AQUAR BALTIMORE MEMPHIS GREENBAY BALTIMORE FORTWORTH KNOXVILLE PITTS CA BOS NEAQ BALTIMORE BOS NEAQ BALTIMORE MINNESOTA NEWPORTKY CINCINNAT LITTLEROC MONTERYAQ TAUTPHAUS MONTERYAQ GATLINBAQ SANDIEGOZ JENKINSON SANDIEGOZ BUSCH TAM CHICAGOLP CALDWELL CHICAGOLP BALTIMORE TAUTPHAUS BALTIMORE CINCINNAT GREENBAY BOS NEAQ GA AQUAR MONTERYAQ MYSTIC BALTIMORE GATLINBAQ DALLAS BALTIMORE DALLAS GA AQUAR BROOKLYN BALTIMORE BOS NEAQ BALTIMORE 93

95 Male Female ID MK Known Age Location ID MK Known Age Location BOS NEAQ ERIE CHICAGOLP JENKINSON MONTERYAQ ADVENTURE BOS NEAQ BALTIMORE CALDWELL MEMPHIS OMAHA BALTIMORE BUSCH TAM AQUARAMER TAUTPHAUS AQUARAMER ROCHESTER MINNESOTA BALTIMORE CINCINNAT TULSA MINNESOTA COLO SPRG GATLINBAQ ST PAUL MONTERYAQ PITTS CA TAUTPHAUS GA AQUAR BALTIMORE MINNESOTA BALTIMORE BUSCH TAM GREEN NSC MINNESOTA MYSTIC BALTIMORE BALTIMORE BALTIMORE FT WAYNE BALTIMORE MADISON FORTWORTH BALTIMORE TORONTO AQUARAMER TORONTO TOLEDO TORONTO BOS NEAQ BUSCH TAM CHICAGOLP GA AQUAR BOS NEAQ ERIE BALTIMORE TULSA MINNESOTA BALTIMORE MINNESOTA MINOT JENKINSON MYSTIC RACINE MYSTIC FT WAYNE MYSTIC MINNESOTA MYSTIC FT WAYNE BROOKLYN FT WAYNE CALDWELL GATLINBAQ BALTIMORE BALTIMORE MONTERYAQ VANCOUVAQ MEMPHIS VANCOUVAQ LITTLEROC ROCHESTER BALTIMORE AQUARAMER CALDWELL MONTERYAQ ROCHESTER BALTIMORE MEMPHIS ADVENTURE MEMPHIS VANCOUVAQ JENKINSON BOS NEAQ GREENBAY VANCOUVAQ BALTIMORE BOS NEAQ AQUARAMER VANCOUVAQ CINCINNAT PITTS CA MINOT MONTERYAQ GATLINBAQ GATLINBAQ BOS NEAQ TOLEDO FT WAYNE BOS NEAQ TULSA LVZOO STEINHART SPRINGFIE BALTIMORE GREEN NSC BALTIMORE GATLINBAQ BALTIMORE GATLINBAQ BALTIMORE LVZOO DALLAS WA GA AQUAR PUEBLO BALTIMORE GATLINBAQ OMAHA JENKINSON OMAHA BALTIMORE CHICAGOLP 94

96 Male Female ID MK Known Age Location ID MK Known Age Location JENKINSON LOWRY JENKINSON NEWPORTKY JENKINSON NEWPORTKY JENKINSON GREEN NSC BALTIMORE GREEN NSC BALTIMORE FORTWORTH ERIE GREEN NSC W ORANGE CALDWELL FT WAYNE SAGINAW BALTIMORE BALTIMORE DENVER BALTIMORE LITTLEROC GREEN NSC DALLAS OMAHA VANCOUVAQ OMAHA BALTIMORE W ORANGE ST PAUL ERIE W ORANGE MINNESOTA FT WAYNE MINNESOTA FT WAYNE TULSA ROCHESTER MONTERYAQ MINOT ROCHESTER ROCHESTER TORONTO GREEN NSC ST PAUL MINNESOTA AQUARAMER MINNESOTA MONTERYAQ BALTIMORE FORTWORTH CHICAGOLP LVZOO VANCOUVAQ TAMPA AQ MINNESOTA DENVER SAGINAW DENVER BALTIMORE COLO SPRG AQUARAMER CALDWELL CALDWELL ROCHESTER LITTLEROC NEWPORTKY ERIE MONTERYAQ BROOKLYN BALTIMORE RACINE BROOKLYN BALTIMORE FORTWORTH GA AQUAR W ORANGE TOLEDO SPRINGFIE OMAHA TAMPA AQ TOLEDO ROCHESTER TAMPA AQ DALLAS GREEN NSC DALLAS GREEN NSC ROCHESTER STEINHART CINCINNAT ROCHESTER BOS NEAQ BROOKLYN ADVENTURE FORTWORTH BOS NEAQ TOLEDO CHICAGOLP SAGINAW LITTLEROC TORONTO LITTLEROC GREENBAY LITTLEROC DALLAS WA BOS NEAQ BALTIMORE ERIE BUSCH TAM TORONTO OMAHA BALTIMORE GREENBAY FT WAYNE KNOXVILLE GREEN NSC COLO SPRG BOS NEAQ TOLEDO DENVER TULSA FORTWORTH RACINE AQUARAMER MINOT RACINE BOS NEAQ BROOKLYN TORONTO GA AQUAR 95

97 Male Female ID MK Known Age Location ID MK Known Age Location TORONTO GREEN NSC BALTIMORE FORTWORTH LITTLEROC ROCHESTER LITTLEROC JENKINSON CINCINNAT ST PAUL TAMPA AQ MEMPHIS OMAHA PITTS CA FT WAYNE FORTWORTH BALTIMORE FORTWORTH AQUARAMER BALTIMORE DALLAS ROCHESTER DENVER BROOKLYN DENVER DENVER DENVER DALLAS RACINE DENVER CALDWELL TAMPA AQ FORTWORTH COLO SPRG TOLEDO COLO SPRG ROCHESTER DALLAS ROCHESTER DENVER PITTS CA DALLAS AQUARAMER MINNESOTA COLUMBUS BROOKLYN COLUMBUS PUEBLO DALLAS WA STEINHART FT WAYNE STEINHART ROCHESTER ADVENTURE ROCHESTER BALTIMORE JENKINSON ROCHESTER GREEN NSC COLUMBUS TORONTO GREEN NSC MEMPHIS FT WAYNE TORONTO ROCHESTER BOS NEAQ TULSA BALTIMORE BALTIMORE FT WAYNE STEINHART MONTERYAQ GATLINBAQ LITTLEROC PUEBLO GREEN NSC PUEBLO SPRINGFIE MEMPHIS SPRINGFIE TULSA GREEN NSC NEWPORTKY LITTLEROC MINNESOTA BOS NEAQ MINNESOTA BOS NEAQ ADVENTURE GATLINBAQ ROCHESTER FORTWORTH ADVENTURE BOS NEAQ ADVENTURE PUEBLO ROCHESTER AQUARAMER BOS NEAQ AQUARAMER MEMPHIS AQUARAMER BALTIMORE RACINE SANDIEGOZ TOLEDO FT WAYNE SAGINAW W ORANGE BROOKLYN ADVENTURE TAUTPHAUS ADVENTURE STEINHART KNOXVILLE W ORANGE MEMPHIS ROCHESTER MEMPHIS ROCHESTER SPRINGFIE JENKINSON DENVER OMAHA ROCHESTER BALTIMORE ROCHESTER GREEN NSC ERIE COLO SPRG ROCHESTER 96

98 Male Female ID MK Known Age Location ID MK Known Age Location W ORANGE BROOKLYN CALDWELL ST PAUL LVZOO CHICAGOLP FORTWORTH GA AQUAR MEMPHIS BOS NEAQ FORTWORTH FORTWORTH W ORANGE GATLINBAQ TAMPA AQ AQUARAMER DENVER DENVER DENVER DALLAS WA DALLAS WA LVZOO LVZOO BALTIMORE BALTIMORE ROCHESTER STEINHART LITTLEROC GREENBAY LOWRY LVZOO FT WAYNE LITTLEROC FT WAYNE TOLEDO GREEN NSC TULSA FT WAYNE FT WAYNE TOLEDO OMAHA OMAHA ADVENTURE ADVENTURE MINNESOTA ROCHESTER PITTS CA ADVENTURE ROCHESTER ADVENTURE ROCHESTER ERIE MEMPHIS DALLAS COLUMBUS COLUMBUS MEMPHIS MEMPHIS STEINHART SANDIEGOZ LOWRY PUEBLO STEINHART STEINHART STEINHART STEINHART ROCHESTER 97

99 Male Female ID MK Known Age Location ID MK Known Age Location GATLINBAQ ADVENTURE ADVENTURE SANDIEGOZ ERIE RACINE KNOXVILLE ADVENTURE TULSA TORONTO BALTIMORE LITTLEROC ADVENTURE 98

100 REPORT OVERVIEW: Appendix F Descriptive Survival Statistics Report AFRICAN PENGUIN Studbook SPHENISCUS DEMERSUS North American Regional Studbook Studbook data current as of 4/1/2016 Compiled by Seana Flossic sflossic@tulsazoo.org PopLink Studbook filename: AfricanPenguin_6july2016_13Jul16 PopLink User Who Exported Report: ssullivan Date of Export: 8/9/2016 Data Filtered by: Association = AZA.FED AND StartDate = 1/1/1970 AND EndDate = 8/9/2016 PopLink Version: 2.4 Based on this analysis, if an AFRICAN PENGUIN survives to its first birthday, its median life expectancy is 18.6 years for males and 15.3 years for females. Please see the body of the report for more details. BACKGROUND ON ANALYSES: These analyses were conducted using animals that lived during the period 1 January 1970 to 9 August 2016 at institutions within AZA. The analyses mainly focus on survival statistics from 1 year (e.g. excluding any individuals that did not survive past their first birthday). These statistics most accurately reflect typical survival for animals which can be seen on exhibit in zoos and aquariums. This report summarizes survival records of individuals housed at zoological facilities for a specific geographic range and time period; these records trace an individual's history from birth or entry into the population to death, exit out of the population, or the end of the time period. As such, this history only reflects standard practices - including management, husbandry, and acquisition/disposition practices - for the specified time period and geographic range. Thus, the report contents should be viewed with some caution as they may not fully reflect current and newly emerging zoo and aquarium management techniques or practices. For example, if the population has not been maintained in zoos and aquariums long enough to have many adults living into old age, median life expectancy will likely be an underestimate until more data accrue in older age classes. Thus, users of these reports should recognize that the results produced will likely vary over time or depending on the subset of data selected. For many species, including humans, survival statistics often differ for males and females. For this population, male and female data were robust enough to detect statistical differences 1 in survival statistics. These results therefore include separate statistics for males and females; individuals whose sex is unknown are proportionally included in calculations. SUMMARY OF ANALYSES: SURVIVAL STATISTICS Female # of individuals with partial or full lifespans used in analysis # of individuals which had died by 9 August 2016 (analysis end date) % of individuals which had died by 9 August % 37.7% median life expectancy assuming an individual survives to its first birthday (years) % confidence interval for median (years) 14.5 to to 20.0 only 25% of animals live beyond this age (years) First-year (infant) survival 74.3% 63.8% maximum longevity (years) See footnotes 1, 2, 3, 4, and 5 for definitions of terminology used in this table. Male 99

101 The median life expectancy 2 for AFRICAN PENGUIN was statistically different between males and females, with males having a significantly higher median life expectancy. Given the quality of the data - how many animals are in the database and how many have died - there is a 95% chance that the true median for each sex falls within the 95% confidence intervals displayed in the table. The table also lists the ages which only 25% of AFRICAN PENGUIN can be expected to survive past and the first-year (infant) survival 3 rates. The year after birth/hatching is a period of relatively low survival for many species and life histories. The maximum longevity 4 observed for AFRICAN PENGUIN females is based on an individual which was DEAD as of the analysis end date (studbook number 188, sex = Female, origin = Captive Hatch, birth date estimate = +/- 1 Year). The maximum longevity observed for AFRICAN PENGUIN males is based on an individual which was DEAD as of the analysis end date (studbook number 70, sex = Male, origin = Wild Hatch, birth date estimate = +/- 1 Year). 5 The correct interpretation of these statistics is that, if it survives the first year of life, the 'typical' male AFRICAN PENGUIN will live 18.6 years and the typical female will live 15.3 years; that half of all AFRICAN PENGUIN can be expected to die before they reach the male and female median life expectancies and half will live longer than that; that only 25% of all male AFRICAN PENGUIN can be expected to live to 28.4 years and females to 27.2 years; and that it is rare but possible for male AFRICAN PENGUIN to live 45.7 years and female AFRICAN PENGUIN to live 41.1 years. The median life expectancy, confidence interval, first-year survival, and maximum longevity may change as more data are accumulated, the population's age structure changes, or management practices improve. While both median life expectancy and maximum longevity are discussed in this report, it is more appropriate to rely on median life expectancy to place the age of any one individual in context. To put these statistics in perspective, median life expectancy from age one for people in the United States is 77.5 years and the maximum longevity (documented worldwide) is 122 years 6. Therefore, if a person lived to be 85 years old, the appropriate context is that they lived well beyond the median life expectancy (77.5), not that they fell short of the maximum longevity (122). DATA QUALITY The PopLink Survival Tool uses five data quality measures to determine whether data are robust enough to make reliable estimates of key survival parameters. The subsets of male and female data for this population passed all of the following data quality tests: 1. Can the median life expectancy be calculated? PASS 2. Is the sample size (number of individuals at risk) greater than 20 individuals at the median? PASS 3. Is the 95% Confidence Interval (CI) bounded? PASS 4. Is the sample size in the first age class of analysis (e.g. the first day of analysis) greater than 30 individuals? PASS 5. Is the length of the 95% CI < 33% of the maximum longevity? PASS 1 Statistical significance was determined by comparing 84% confidence intervals around median life expectancy for males and females, with 311 unknown sex individuals proportionally incorporated into the analysis. For this population, non-overlapping confidence intervals indicated that male and female medians were significantly different. See the PopLink manual for more details. 2 The statistics analyzed for this report (median life expectancy, 95% confidence limits, and age to which 25% of individuals survive) exclude any individuals who did not survive to their first birthday; these individuals are excluded because this Report is focused on providing median survival estimates for the typical individual that survives the vulnerable infant stage. In other words, this report answers the question, 'how long is this species expected to live once it has reached its first birthday?' For this studbook, 878 individuals died before their first birthday and were excluded from these analyses. For all animals that survive to their first birthday, 50% will die before the median life expectancy in this report and 50% die after. Note that the median life expectancy obtained from population management software (PM2000, PMx, ZooRisk) or from life tables in Breeding and Transfer Plans (e.g. where Lx = 0.5) will be lower because it includes these individuals that did not survive to their first birthday in order to project the correct number of births needed. See the PopLink manual for more details. 3 For reference, first-year survival is provided. For this studbook and the selected demographic window, 878 individuals did not survive to their first birthday and were excluded from the estimates provided above (median life expectancy, 95% confidence limits, and age to which 25% of individuals survive). 4 Maximum longevity is the age of the oldest known individual for this species, living or dead. It is not necessarily the biological maximum age, but only reflects the individuals included in the dataset. 5 Censored individuals are individuals whose deaths have not been observed as of the end of the analysis window, including individuals who 1) are still alive as of the end date, 2) exited the geographic window before the end date (through transfer or release), or 3) were lost-to-follow up before the end date. 6 Median life expectancy for people is estimated from: Xu, Jiaquan, Kochanek KD, Murphy SL, and Tejada-Vera B Deaths: Final Data for National vital statistics reports; vol 58 no 19. Hyattsville, MD: National Center for Health Statistics. Jeanne Calment of France was the oldest documented and fully validated human and died at 122 years and 164 days; from: Accessed August 9,

102 Appendix G Reproductive Report AFRICAN PENGUIN Studbook SPHENISCUS DEMERSUS North American Regional Studbook Studbook data current as of 4/1/2016 Compiled by Seana Flossic sflossic@tulsazoo.org PopLink Studbook filename: AfricanPenguin_6july2016_13Jul16 PopLink User Who Exported Report: ssullivan Date of Export: 8/9/2016 PopLink Version: 2.4 Species Type: Egg Laying Incubation Period: 38 Days Maximum Hatch Date Range For Clutch Mates: 8 Days DAM INFORMATION 436 reported dams, with (3153) offspring (not including 170 offspring of UNK/MULT dams) Median size: 1 Mean size: Clutch Size Frequency Percentage Total Dam Age at First Reproduction Median age: Mean age: Youngest Dams at First Reproduction: Studbook ID Age At Hatch Dam's Hatch Date Estimate First Offspring's ID First Offspring's Hatch Date /11/1991 None /25/1992 None /1/1976 +/- 1 Year 303 7/6/1977 None /15/1975 None /6/1977 None /15/1975 None /10/1977 None /23/1980 None 542 3/1/1982 None First Offspring's Estimate 5 Oldest Dams at First Reproduction: Studbook ID Age At Hatch Dam's Hatch Date Estimate First Offspring's ID First Offspring's Hatch Date /13/1987 None /10/2011 None /2/1992 None /11/2016 None /23/1992 None /23/2014 None /15/1975 None /16/1996 None /21/1975 None /27/1996 None /13/1974 None /3/1991 None First Offspring's Estimate 101

103 Dam Age for All Reproduction Median age: Mean age: Oldest Dams to Have Reproduced Studbook ID Age At Hatch Dam's Hatch Date Estimate Offspring's ID Offspring's Hatch Date Offspring's Estimate /28/1980 None /30/2012 None /28/1980 None /3/2009 None /19/1987 None /28/2016 None /5/1981 None /17/2010 None /19/1984 None /14/2012 None Hatch Seasonality First of clutch must have a hatch date estimate of None, Day, or Month to be counted. Month Number of Clutches Percentage January February March April May June July August September October November December Total SIRE INFORMATION 434 reported sires, with (3160) offspring (All ages are at dam conception) Sire Age at First Reproduction Median age: Mean age: Youngest Sires at First Reproduction: Studbook ID Age At Estimated Conception Sire's Hatch Date Estimate First Offspring's ID First Offspring's Hatch Date /9/1990 None /22/1991 None /31/1973 +/- 1 Year 230 9/5/1975 None /24/1981 None 632 3/2/1983 None /15/1975 None /10/1977 None /14/1981 None 609 1/10/1983 None First Offspring's Estimate 102

104 5 Oldest Sires at First Reproduction: Studbook ID Age At Estimated Conception Sire's Hatch Date Estimate First Offspring's ID First Offspring's Hatch Date /19/1985 None /4/2013 None /30/1993 None /7/2015 None /17/1991 None /6/2013 None /3/1966 +/- 1 Year /26/1987 None /4/1991 None /14/2013 None First Offspring's Estimate Sire Age for All Reproduction Median age: Mean age: Oldest Sires to Have Reproduced Studbook ID Age At Estimated Conception Sire's Hatch Date Estimate Offspring's ID Offspring's Hatch Date Offspring's Estimate /11/1975 None /14/2011 None /11/1975 None /25/2010 None /11/1975 None /4/2009 None /11/1975 None /25/2008 None /11/1975 None /8/2007 None 103

105 Appendix H Population Viability Analysis (PVA) 2014 Summary Report African Penguin (Spheniscus demersus) July 2014 Population Biologist: Nicole Clausen, nclausen@lpzoo.org Studbook Keeper: Seana Flossic, sflossic@wcs.org SSP Coordinator: Steven Sarro, sarros@si.edu AZA Penguin TAG Chair: Tom Schneider, tschneider@detroitzoo.org AZA Photograph courtesy of Steve Sarro, Smithsonian National Zoological Park, 2014 Projected zoo & aquarium population status in 100 years OUT potential changes: potential changes: Current Population Status 835 penguins (442 males, 388 females, 5 unknown) are housed at 48 AZA institutions The population has increased at 3.8% annual growth (λ) over the last decade, with an average of ~55 hatches/year 98% of the founding gene diversity (GD) has been retained The population s average inbreeding value (F) is IUCN status: Endangered Age pyramid of the AZA African penguin population Model Results Projected Status OUT Potential Changes If the population continues breeding at current rates and continues current rates of imports and exports, it would decline to a size of 625 ± 94 individuals in 100 years. GD is predicted to be maintained at 99.5% ± 0.05% in 100 years with low inbreeding levels ( ± 0.002) Status: Low Risk in zoos & aquariums in 100 years For additional details, see page 7 in the main report Projected Status Potential Changes The population could fulfill institutional interest if the breeding rate is increased to ~68 hatches/year with imports and exports High gene diversity (GD: 99.7% ± 0.02%) and low inbreeding (F: ± 0.001) would be maintained Status: Low Risk in zoos & aquariums in 100 years For additional details, see pages 8-9 in the main report Essential Management Actions How Institutions Can Help Increase breeding to produce ~68 hatches/year to avoid decline and fulfill institutional interest Pursue breeding recommendations given to your institution Report breeding challenges and successes to the Program Leader 104

106 EXECUTIVE SUMMARY Population Viability Analyses (PVA) are being conducted by Lincoln Park Zoo and Population Management Center researchers through funding from the Institute of Museum and Library Services (IMLS). The project team uses ZooRisk 3.80 (Earnhardt et al. 2008), a PVA modeling software, to examine what would happen to AZA populations if current conditions remain the same (the baseline scenario), and then assess the impact of changes in reproductive rates, space availability, imports/exports, and other potential management actions (alternate scenarios). Model scenarios for this population were developed with members of the Association of Zoos and Aquarium (AZA) Penguin Taxonomic Advisory Group (TAG) during winter of POPULATION HISTORY/CURRENT STATUS African penguins (Spheniscus demersus) have been consistently held in AZA institutions since Since that time, the African penguin population has grown through a combination of zoo hatches and importations of animals from outside of AZA, reaching a peak of 857 individuals in Currently, the population has 835 animals housed at 48 institutions. In the past 10 years, annual hatch rates have fluctuated quite dramatically. The population has averaged ~55 hatches/year over the past decade and ~66 hatches/year over the past five years in particular. Over the last 10 years, the AZA African penguin population has experienced an average of 13.6 imports and 8.7 exports per year. The population currently has high gene diversity (98.8%) and low inbreeding (average inbreeding coefficient of ). PVA RESULTS Model results indicate that if the current breeding rate (~55 hatches/year in the past 10 years) continues, the AZA African penguin population will decline over the next 100 years, regardless of whether it is open to future imports and exports. However, increasing the breeding rate will allow the population to remain stable or fill potential spaces (1100) over the next 100 years. If imports and exports continue in the future, the population needs to produce 60 hatches per year to remain at its current size or 68 per year to fill space. However, if the population is closed to future imports and exports, it will need to produce 67 hatches per year to remain at its current size or 76 per year to fill space. Increased breeding rates (with or without imports and exports) will also maintain the population s genetic health over the next century. MANAGEMENT ACTIONS The AZA African Penguin Animal Program should consider the follow changes to current management strategies: Increase breeding rates: If the population remains open to imports and exports, breeding should be increased from 55 to a minimum of 60 hatches each year to maintain the population at its current size or 68 hatches per year to fill spaces. If the population is closed to imports and exports, breeding needs to increase further (67 hatches/year to stabilize or 76 hatches/year to fill spaces). If elevated breeding rates can be maintained, the population may become demographically stable and maintain its genetic health. Please see the 2014 African Penguin AZA Animal Program full PVA report on the AZA website for further details. Contact Brent Johnson at bjohnson@lpzoo.org with questions 105

107 Appendix I African Penguin SSP Submission Form for Gender Testing Date: Submitting Institution: Address: Sample Contact Person: Phone/Fax/or for results: Send Invoice to: Species/common name: Protocol for submitting blood, feather or tissue sample for DNA analysis: 1. Call or Jean Dubach BEFORE SHIPPING to schedule the arrival of the sample in the Wildlife genetics lab. PHONE: (708) jeandubach@gmail.com 2. Blood or tissue samples, collect up to 0.25 mls of blood or a pea sized piece of tissue. However, a drop of blood or clots will work, stored in most preservatives or clot tubes. Leftover blood from other lab tests can be used. - Samples can be stored in the refrigerator for one or two days prior to shipment and shipped at ambient temperature or frozen prior to shipment for longer storage then shipped frozen. - Label tube with the date, studbook #, and house ID#. Secure with tape or parafilm. Wrap each tube with a little paper towel to keep them from breaking. - Package in an insulated container. In hot weather include a freezer pack to keep samples from getting hot. FedEx overnight delivery to: Jean Dubach Loyola University Medical Center 2160 South First Avenue CTRE, First Flood, Dock 8 Maywood, IL Feathers: Place feathers from each individual in a baggy or envelop. They can be shipped through regular mail to the following address: Jean Dubach Loyola University Medical Center 2160 S. First Avenue Attn: CTRE, First Floor, Dock 8 Maywood, IL Fill out this form and include it in the envelop or package. 5. Charge for sexing birds is $20 per individual. Studbook # ISIS/ID Test requested: sex / species Sex Results Please inform the African penguin Studbook Keeper of the results: Seana Flossic, Tulsa Zoo, sflossic@tulsazoo.org 106